Click here to switch to the map view.

The map label for this gene is cusS

Identifier: 218698942

GI number: 218698942

Start: 573140

End: 574582

Strand: Reverse

Name: cusS

Synonym: ECIAI39_0545

Alternate gene names: 218698942

Gene position: 574582-573140 (Counterclockwise)

Preceding gene: 218698943

Following gene: 218698941

Centisome position: 11.2

GC content: 52.18

Gene sequence:

>1443_bases
ATGGTCAGTAAGCCATTTCAGCGCCCGTTTTCGCTGGCAACACGCCTGACCTTTTTTATCAGCCTGGCGACCATCGCGGC
GTTTTTCGCCTTTGCCTGGATCATGATCCACTCGGTAAAAGTGCATTTTGCCGAGCAGGATATTAATGATTTAAAAGAGA
TTAGCGCCACCCTTGAACGGGTACTTAATCACCCTGACGAAACGCAAGCCCGACGCTTAATGACGCTGGAAGATATTGTC
AGTGGTTATTCCAATGTGTTGATCTCCCTGACAGATAGCCACGGTAAAACGGTGTATCACTCCCCCGGTGCGCCGGATAT
CCGCGAGTTTACGCGTGACGCCATACCCGATAAAGACACGCGGGGCGGTGAGGTGTATCTCCTTTCCGGCCCGACGATTA
TGATGCCGGGCCACGGTCACGGGCATATGGAACACAGCAACTGGCGGATGATTAACTTGCCGGTTGGCCCGCTGGTGGAC
GGCAAACCGATTTATACGCTCTACATCGCGCTGTCTATCGATTTTCATCTTCATTACATTAATGATTTGATGAATAAACT
TATTATGACCGCATCGGTAATCAGCATCCTGATCGTCTTTATCGTGCTGCTGGCGGTACATAAAGGCCACGCGCCAATTC
GCAGCGTCAGCCGTCAAATCCAGAACATTACCTCGAAAGATCTCGACGTTCGTCTCGATCCACAGACCGTGCCCATTGAA
CTCGAACAACTGGTACTGTCGTTCAACCATATGATCGAGCGTATTGAGGATGTCTTTACCCGCCAGTCCAATTTCTCAGC
GGATATCGCCCACGAAATTCGCACACCAATTACGAATCTCATAACGCAAACGGAAATCGCCCTCAGCCAGTCGCGCAGCC
AGAAGGAGCTGGAAGATGTGCTCTACTCTAATCTCGAAGAGCTGACGCGAATGGCGAAAATGGTCAGCGATATGTTGTTT
CTCGCCCAGGCCGATAACAACCAGTTAATCCCCGAAAAGAAAATGCTCAACCTGGCGGATGAAGTCGGCAAAGTGTTCGA
TTTTTTCGAGGCGTTAGCGGAAGATCGCGGCGTGGAGTTGCGGTTTGTTGGTGACGAGTGTCAGGTTGCGGGCGATCCGC
TGATGTTGCGTCGGGCGTTAAGCAACCTGCTTTCGAACGCCCTGCGTTATACGCCACCCGGAGAGGCAATTGTTGTGCGC
TGCCAGACGGTCGATCACCAGGTGCAAGTTTCCGTCGAAAACCCCGGTACGCCCATTGCGCCCGAACACTTACCGCGATT
GTTTGACCGTTTCTACCGCGTTGATCCTTCCCGCCAGCGAAAAGGCGAAGGTAGCGGCATTGGCCTGGCGATAGTGAAAT
CGATTGTTGTCGCGCATAAAGGCACGGTTGCGGTAACGTCGGATGCGCGGGGGACAAGGTTTGTTATCACATTACCCGCT
TAA

Upstream 100 bases:

>100_bases
GATGTGGCGGTGAAGCGGCTGCGCGGCAAAATCGACAACGACTTTGAGCCGAAGCTGATTCAAACCGTGCGCGGCGTGGG
CTACATGCTTGAGGTGCCGG

Downstream 100 bases:

>100_bases
TCCTTCAGCAAACGGCAACTTTTATAACCAGTGTAAAAATAACGTGCCGCAATAGTCATACCTATTAATGGTAAAAAGCT
GTCACAATTCATAAAAAACC

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLTDSHGKTVYHSPGAPDIREFTRDAIPDKDTRGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTPPGEAIVVR
CQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVITLPA

Sequences:

>Translated_480_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLTDSHGKTVYHSPGAPDIREFTRDAIPDKDTRGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTPPGEAIVVR
CQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVITLPA
>Mature_480_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLTDSHGKTVYHSPGAPDIREFTRDAIPDKDTRGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTPPGEAIVVR
CQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVITLPA

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=480, Percent_Identity=97.9166666666667, Blast_Score=948, Evalue=0.0,
Organism=Escherichia coli, GI1788279, Length=296, Percent_Identity=33.7837837837838, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1786600, Length=263, Percent_Identity=33.8403041825095, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=30.2583025830258, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1790346, Length=332, Percent_Identity=29.5180722891566, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1787894, Length=311, Percent_Identity=29.2604501607717, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789403, Length=282, Percent_Identity=31.2056737588652, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693157, Length=237, Percent_Identity=27.0042194092827, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1790551, Length=303, Percent_Identity=28.0528052805281, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1789808, Length=283, Percent_Identity=27.5618374558304, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=27.9661016949153, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081816, Length=251, Percent_Identity=27.4900398406374, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=26.3636363636364, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=28.695652173913, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790861, Length=298, Percent_Identity=25.503355704698, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787374, Length=340, Percent_Identity=22.0588235294118, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI48994928, Length=240, Percent_Identity=26.25, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082128, Length=296, Percent_Identity=24.3243243243243, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786912, Length=242, Percent_Identity=26.0330578512397, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788549, Length=223, Percent_Identity=26.457399103139, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53774; Mature: 53774

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHH
VLNHPDETQARRLMTLEDIVSGYSNVLISLTDSHGKTVYHSPGAPDIREFTRDAIPDKDT
HHCCCCHHHHHHHHHHHHHHCCHHCEEEEEECCCCCEEEECCCCCHHHHHHHHCCCCCCC
RGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFHLHYI
CCCEEEEEECCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEHHHHHH
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHH
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RFVGDECQVAGDPLMLRRALSNLLSNALRYTPPGEAIVVRCQTVDHQVQVSVENPGTPIA
EEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEECCCCCCCC
PEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVITLPA
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC
>Mature Secondary Structure
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHH
VLNHPDETQARRLMTLEDIVSGYSNVLISLTDSHGKTVYHSPGAPDIREFTRDAIPDKDT
HHCCCCHHHHHHHHHHHHHHCCHHCEEEEEECCCCCEEEECCCCCHHHHHHHHCCCCCCC
RGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFHLHYI
CCCEEEEEECCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEHHHHHH
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHH
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RFVGDECQVAGDPLMLRRALSNLLSNALRYTPPGEAIVVRCQTVDHQVQVSVENPGTPIA
EEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEECCCCCCCC
PEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVITLPA
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]