Click here to switch to the map view.

The map label for this gene is yahF

Identifier: 218698776

GI number: 218698776

Start: 399343

End: 400890

Strand: Reverse

Name: yahF

Synonym: ECIAI39_0366

Alternate gene names: 218698776

Gene position: 400890-399343 (Counterclockwise)

Preceding gene: 218698777

Following gene: 218698775

Centisome position: 7.81

GC content: 54.84

Gene sequence:

>1548_bases
ATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAA
ACTCGACGGCGTCGAGCAGGCATTTGTGGCGATGGCGACCGAAATGAACAAAGGCGTGCTGAAGAATTTAGGGCTGCTGA
CGCCGGAACTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAACGGCAAAGCGGGCGCAGACAACGAGCAGTTG
CTGGTGGAGATTGAAGAACTGTTCAACACCAAAGCTCAAACTGGTTCACATGAAGCACGCTATGCCACTATTGCCAGCGC
CAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGC
TGCAAAACGATCTTAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAACAGTTGGCCCAC
GAAAAAGGGCTGCTGATGATGGGGCCAGACTGCGGTACGGCGATTATCAACGGCGCGGCGCTCTGCTTTGGTAACGCCGT
GCGTCGCGGCAACATCGGCATTGTTGGCGCATCTGGCACCGGCAGCCAGGAACTGAGCGTCCGTATCCATGAATTTTGCG
GCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGTCTGATGATGCTCGATGCCATC
GGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGTGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGT
GCTGGAACGCGCACGTGCCTGCCGCAAGCCGGTGGTGGTCTGCTTCCTCGGTCGTGGCGAAACGCCAGTGGATGAGCAGG
GGCTACAGTTTGCTCGCGGCAGCAAAGAAGCGGCGCTGAAAGCGGTAATGCTCTCCGGCGTGAAACAGGAACATCTCGAT
CTGCATACCCTGAACCAGCCATTGATTGCTGACGTGCGCGCTCGCCTGAAGCCACAACAGAAATACATTCGTGGCCTGTT
CTGCGGCGGTACGCTGTGTGACGAAACCATGTTCGCGGTGATGGAAAAACACGGCGATGTCTACAGCAACATCCAGCCCG
ATCCTGAATTCCGCCTGAAAGATATCAACCGCAGCGTCAAACATACCTTCCTCGACTTTGGCGATGACGATTTTACCAAC
GGCAAGCCGCATCCAATGATTGACCCCACCAACCGTATCAGCCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGT
GATCGTGATGGATTTTGTGCTCGGGTTTGGATCGCATGAAGATCCGGTCGGTTCCACCATCGAGGCGATCAAAGAGGCGA
AAGCGATCGCTGCCGCCGATGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCATTA
GAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGA
ATTTATCTGCAAAGGGGAGGAAGCCTGA

Upstream 100 bases:

>100_bases
GCAATAGAAAAAATTAAACATATTGGCTCCAGTTCCGGCTGCGACATGCTGTATGGCATGGCCGATGGTTGTGCGCTGAG
CCAAACCTACGGAGGGAATT

Downstream 100 bases:

>100_bases
TGAGCCAGTCACTGTTTAGCCAACCGTTGAACGTTATTAACGTCGGCATCGCCATGTTTAGCGATGATCTGAAAAAGCAG
CATGTAGAAGTGACCCAACT

Product: putative enzyme with acyl-CoA domain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNKGVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQL
LVEIEELFNTKAQTGSHEARYATIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFCGGVSQLIGTGGRDLSEKIGGLMMLDAI
GMLENDPQTEIIVLISKPPAPAVARKVLERARACRKPVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLD
LHTLNQPLIADVRARLKPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSVKHTFLDFGDDDFTN
GKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAIKEAKAIAAADGRELIILAYVLGTDLDTPSL
EQQSQMLLDAGVILASSSTNTGLLAREFICKGEEA

Sequences:

>Translated_515_residues
MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNKGVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQL
LVEIEELFNTKAQTGSHEARYATIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFCGGVSQLIGTGGRDLSEKIGGLMMLDAI
GMLENDPQTEIIVLISKPPAPAVARKVLERARACRKPVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLD
LHTLNQPLIADVRARLKPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSVKHTFLDFGDDDFTN
GKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAIKEAKAIAAADGRELIILAYVLGTDLDTPSL
EQQSQMLLDAGVILASSSTNTGLLAREFICKGEEA
>Mature_514_residues
SVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNKGVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLL
VEIEELFNTKAQTGSHEARYATIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAHE
KGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFCGGVSQLIGTGGRDLSEKIGGLMMLDAIG
MLENDPQTEIIVLISKPPAPAVARKVLERARACRKPVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDL
HTLNQPLIADVRARLKPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSVKHTFLDFGDDDFTNG
KPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAIKEAKAIAAADGRELIILAYVLGTDLDTPSLE
QQSQMLLDAGVILASSSTNTGLLAREFICKGEEA

Specific function: Unknown

COG id: COG0074

COG function: function code C; Succinyl-CoA synthetase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli fdrA and some, to bacterial sucD [H]

Homologues:

Organism=Escherichia coli, GI1786513, Length=515, Percent_Identity=97.6699029126214, Blast_Score=1007, Evalue=0.0,
Organism=Escherichia coli, GI1786729, Length=513, Percent_Identity=38.5964912280702, Blast_Score=304, Evalue=7e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003781
- InterPro:   IPR005811
- InterPro:   IPR016102 [H]

Pfam domain/function: PF02629 CoA_binding; PF00549 Ligase_CoA [H]

EC number: NA

Molecular weight: Translated: 55561; Mature: 55429

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNKGVLKNLGLLTPELEQA
CCEEEEECCCCCCCCEEEEEEHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
KNGDLMIVINGKAGADNEQLLVEIEELFNTKAQTGSHEARYATIASAKKHIPESNLAVIS
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
VNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAHEKGLLMMGPDCGTAIINGAA
ECCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHCCHH
LCFGNAVRRGNIGIVGASGTGSQELSVRIHEFCGGVSQLIGTGGRDLSEKIGGLMMLDAI
HHHCCCEECCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCEEEEHHH
GMLENDPQTEIIVLISKPPAPAVARKVLERARACRKPVVVCFLGRGETPVDEQGLQFARG
HHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHCHHHHCC
SKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRARLKPQQKYIRGLFCGGTLCDETMFAV
CHHHHHHHHHHHCCHHHHCHHHHCCCHHHHHHHHHCCHHHHHHHEEECCCHHHHHHHHHH
MEKHGDVYSNIQPDPEFRLKDINRSVKHTFLDFGDDDFTNGKPHPMIDPTNRISRLIEEA
HHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RDPEVAVIVMDFVLGFGSHEDPVGSTIEAIKEAKAIAAADGRELIILAYVLGTDLDTPSL
CCCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCH
EQQSQMLLDAGVILASSSTNTGLLAREFICKGEEA
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNKGVLKNLGLLTPELEQA
CEEEEECCCCCCCCEEEEEEHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
KNGDLMIVINGKAGADNEQLLVEIEELFNTKAQTGSHEARYATIASAKKHIPESNLAVIS
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
VNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAHEKGLLMMGPDCGTAIINGAA
ECCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHCCHH
LCFGNAVRRGNIGIVGASGTGSQELSVRIHEFCGGVSQLIGTGGRDLSEKIGGLMMLDAI
HHHCCCEECCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCEEEEHHH
GMLENDPQTEIIVLISKPPAPAVARKVLERARACRKPVVVCFLGRGETPVDEQGLQFARG
HHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHCHHHHCC
SKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRARLKPQQKYIRGLFCGGTLCDETMFAV
CHHHHHHHHHHHCCHHHHCHHHHCCCHHHHHHHHHCCHHHHHHHEEECCCHHHHHHHHHH
MEKHGDVYSNIQPDPEFRLKDINRSVKHTFLDFGDDDFTNGKPHPMIDPTNRISRLIEEA
HHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RDPEVAVIVMDFVLGFGSHEDPVGSTIEAIKEAKAIAAADGRELIILAYVLGTDLDTPSL
CCCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCH
EQQSQMLLDAGVILASSSTNTGLLAREFICKGEEA
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]