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The map label for this gene is prpE

Identifier: 218698758

GI number: 218698758

Start: 378579

End: 380465

Strand: Reverse

Name: prpE

Synonym: ECIAI39_0345

Alternate gene names: 218698758

Gene position: 380465-378579 (Counterclockwise)

Preceding gene: 218698759

Following gene: 218698757

Centisome position: 7.41

GC content: 58.13

Gene sequence:

>1887_bases
ATGTCTTTTAGCGAATTTTATCAGCGTTCGATTAACGAACCGGAGAAGTTCTGGGCCGAGCAGGCCCGGCGTATTGACTG
GCAGACGCCCTTTACGCAAACACTCGATCACAGCAATCCGCCGTTTGCCCGTTGGTTTTGTGAAGGCCGAACCAATTTGT
GTCATAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCATTAATTGCCGTCTCTTCGGAAACAGAAGAA
GAGCGCACCTTTACCTTTCGCCAGTTGCATGACGAAGTGAACGCGGTGGCGTCAATGCTGCGCTCACTGGGCGTACGGCG
TGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACCCTGCTGGCCTGCGCGCGCATTGGCGCTA
TTCACTCGGTAGTGTTTGGTGGATTTGCCTCGCACAGCGTGGCGGCGCGGATTGATGACGCTAAACCGGTGCTGATTGTC
TCGGCTGATGCCGGAGCGCGCGGTGGCAAAATCATTCCTTATAAAAAATTGCTCGACGATGCGATAAGTCAGGCGCAGCA
TCAGCCGCGCCACGTTTTACTGGTGGATCGCGGGCTGGCGAAAATGGCACGCGTCAGCGGGCGGGATGTCGATTTCGCGT
CGTTGCGCCATCAACACATCGGCGCGCGGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACC
TCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTTGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACAC
CATTTTTGGCGGCAAAGCGGGCGGTGTGTTCTTCTGTGCGTCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTCT
ACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTACCGACCTGGCCGGACTGCGGCGTGTGGTGGAAA
ATCGTCGAGAAATATCAGGTGAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGA
AATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGG
TGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGC
GGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGCGTGCCGATGTATGGCTATAACGTGCAGTTGCTTAATGAAGT
CACCGGCGAACCGTGTGGCGTCAACGAGAAAGGGATGCTGGTGGTGGAAGGGCCGCTGCCGCCGGGGTGTATTCAGACCA
TCTGGGGCGACGACGACCGCTTTGTGAAGACGTACTGGTCGCTGTTTTCCCGCCCGGTGTACGCCACCTTTGACTGGGGC
ATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGAC
GCGCGAGATTGAAGAGAGTATCTCCAGTCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAG
GGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAGGTGGCGCATTCGCAAGAGAAG
GCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAA
AACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTG
ATGATCCAGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAG

Upstream 100 bases:

>100_bases
CGACAGAACTCGCCTGGAACAGATGCCGGTCAATGAGTATCTCGACCTGTACGTCATTTAAGCAAACAGCGGTAAGGCGT
AAGTTCAACAGGAGAGCATT

Downstream 100 bases:

>100_bases
GTTATTGTCGGATGCGTCGCCTGGCGCATCCGACACCATGCGCCGATGCCTGATGCGACGCTTACCGCGTCTTATTATGC
CTACCTACTGTTTTTACACC

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEE
ERTFTFRQLHDEVNAVASMLRSLGVRRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYT
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWK
IVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIAR
GLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDDRFVKTYWSLFSRPVYATFDWG
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEK
AIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQAMEE

Sequences:

>Translated_628_residues
MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEE
ERTFTFRQLHDEVNAVASMLRSLGVRRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYT
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWK
IVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIAR
GLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDDRFVKTYWSLFSRPVYATFDWG
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEK
AIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQAMEE
>Mature_627_residues
SFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEE
RTFTFRQLHDEVNAVASMLRSLGVRRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIVS
ADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYTS
GTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKI
VEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARG
LDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDDRFVKTYWSLFSRPVYATFDWGI
RDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKA
IMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQAMEE

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=633, Percent_Identity=39.9684044233807, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI8923896, Length=657, Percent_Identity=35.1598173515982, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI28416953, Length=640, Percent_Identity=36.5625, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI253970489, Length=670, Percent_Identity=34.9253731343284, Blast_Score=391, Evalue=1e-108,
Organism=Homo sapiens, GI115511026, Length=564, Percent_Identity=27.3049645390071, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI42544132, Length=557, Percent_Identity=26.032315978456, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI122937307, Length=586, Percent_Identity=26.962457337884, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI58082049, Length=569, Percent_Identity=26.5377855887522, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI31982927, Length=641, Percent_Identity=23.5569422776911, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI157311624, Length=568, Percent_Identity=26.7605633802817, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI157311622, Length=568, Percent_Identity=26.7605633802817, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI38505220, Length=558, Percent_Identity=25.6272401433692, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI156151445, Length=577, Percent_Identity=22.7036395147314, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI187761345, Length=411, Percent_Identity=24.8175182481752, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI187761343, Length=411, Percent_Identity=24.8175182481752, Blast_Score=80, Evalue=8e-15,
Organism=Escherichia coli, GI1786529, Length=628, Percent_Identity=99.0445859872611, Blast_Score=1285, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=637, Percent_Identity=36.734693877551, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI221142682, Length=543, Percent_Identity=24.1252302025783, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788107, Length=564, Percent_Identity=24.290780141844, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786810, Length=527, Percent_Identity=23.1499051233397, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI145693145, Length=549, Percent_Identity=21.4936247723133, Blast_Score=93, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71982997, Length=654, Percent_Identity=33.6391437308869, Blast_Score=354, Evalue=9e-98,
Organism=Caenorhabditis elegans, GI71983001, Length=654, Percent_Identity=33.6391437308869, Blast_Score=354, Evalue=9e-98,
Organism=Caenorhabditis elegans, GI17569507, Length=650, Percent_Identity=21.8461538461538, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=549, Percent_Identity=25.3187613843352, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=568, Percent_Identity=22.7112676056338, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=582, Percent_Identity=22.3367697594502, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=536, Percent_Identity=22.2014925373134, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6319264, Length=633, Percent_Identity=39.0205371248025, Blast_Score=428, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6323182, Length=641, Percent_Identity=37.597503900156, Blast_Score=422, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319699, Length=407, Percent_Identity=22.6044226044226, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355225, Length=641, Percent_Identity=38.3775351014041, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24667955, Length=650, Percent_Identity=34.9230769230769, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI62472339, Length=562, Percent_Identity=36.2989323843416, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24667959, Length=529, Percent_Identity=35.7277882797732, Blast_Score=332, Evalue=5e-91,
Organism=Drosophila melanogaster, GI21355181, Length=529, Percent_Identity=24.007561436673, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21358303, Length=511, Percent_Identity=23.679060665362, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19922652, Length=545, Percent_Identity=23.8532110091743, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648260, Length=380, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356947, Length=368, Percent_Identity=25, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356441, Length=514, Percent_Identity=23.1517509727626, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281365686, Length=519, Percent_Identity=22.5433526011561, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20130357, Length=389, Percent_Identity=25.1928020565553, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648257, Length=377, Percent_Identity=25.9946949602122, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 69449; Mature: 69318

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDR
CCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHEEECCCCHHHHHHHHH
WLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRSLGVRRGDRVLVYMPMIAE
HHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
AHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIVSADAGARGGKIIPYKKLLDD
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHH
AISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYT
HHHHHHCCCCEEEEECCCHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEE
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAG
CCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCEEEEECCCCHHHHCHHHHHHHHHHH
MATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLY
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCEEEEE
LAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARGLDDRPTRLGSPGVPMYGYN
EECCCCCCCHHHHHHCCCCCCEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCC
VQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDDRFVKTYWSLFSRPVYATFDWG
HHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECC
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAF
CCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCHHHEEEEEHHHHCCCCEEEEE
VIPKESDSLEDREVAHSQEKAIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
ICEGRDPGDLTTIDDPASLDQIRQAMEE
HHCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFARWFCEGRTNLCHNAIDR
CHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHEEECCCCHHHHHHHHH
WLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRSLGVRRGDRVLVYMPMIAE
HHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
AHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIVSADAGARGGKIIPYKKLLDD
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHH
AISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQHIGARVPVAWLESNETSCILYT
HHHHHHCCCCEEEEECCCHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCEEEEEE
SGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGVFFCASDIGWVVGHSYIVYAPLLAG
CCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCEEEEECCCCHHHHCHHHHHHHHHHH
MATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAPTAIRVLKKFPTAEIRKHDLSSLEVLY
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCEEEEE
LAGEPLDEPTASWVSNTLDVPVIDNYWQTESGWPIMAIARGLDDRPTRLGSPGVPMYGYN
EECCCCCCCHHHHHHCCCCCCEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCC
VQLLNEVTGEPCGVNEKGMLVVEGPLPPGCIQTIWGDDDRFVKTYWSLFSRPVYATFDWG
HHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECC
IRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALKGQVAVAF
CCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCHHHEEEEEHHHHCCCCEEEEE
VIPKESDSLEDREVAHSQEKAIMALVDSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
ICEGRDPGDLTTIDDPASLDQIRQAMEE
HHCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]