Click here to switch to the map view.

The map label for this gene is mhpE

Identifier: 218698739

GI number: 218698739

Start: 357164

End: 358177

Strand: Reverse

Name: mhpE

Synonym: ECIAI39_0326

Alternate gene names: 218698739

Gene position: 358177-357164 (Counterclockwise)

Preceding gene: 218698740

Following gene: 218698738

Centisome position: 6.98

GC content: 56.21

Gene sequence:

>1014_bases
ATGAACGATAAAAAACTTTATATCTCGGACGTCACGTTGCGTGATGGTATGCACGCCATTCGTCATCAGTATTCATTGGA
AAACGTTCGCCAGGTTGCCAAAGCACTGGACGATGCCCGCGTGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAG
GTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGCGGCGGCGGATGTGGTGAAGCACGCC
AAAATCGCGACGTTGTTGCTGCCTGGAATCGGCACTATTCACGATCTGAAAAATGCCTGGCAGGCTGGCGCGCGGGTGGT
TCGTGTGGCTACGCACTGTACCGAAGCTGATGTTTCCGCCCAGCATATTCAGTATGCCCGCGAGCTCGGAATGGACACCG
TTGGCTTTCTGATGATGAGCCATATGACCACGCCGGAGAATCTCGCAAAGCAGGCAAAGCTGATGGAAGGCTACGGTGCG
ACCTGTATTTATGTGGTGGATTCTGGCGGTGCGATGAACATGAGCGATATCCGTGACCGTTTCCGCGCCCTGAAAGCAGT
GCTGAAACCAGAAACGCAAACCGGCATACACGCTCACCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGG
AAGAGGGCTGCGACCGAATCGATGCCAGTCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCGCCGCTGGAAGTGTTTATT
GCCGCCGCGGATAAACTGGGTTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCTGACGACCTGGTGCGTCC
GTTGCAGGATCGTCCGGTACGAGTCGATCGCGAAACGCTGGCGCTGGGATACGCCGGTGTTTACTCCAGCTTCCTGCGTC
ACTGTGAAACGGCGGCGGCGCGTTATGGCTTAAGCGCGGTGGATATTCTCGTTGAGTTGGGCAAACGCCGGATGGTTGGC
GGCCAGGAGGATATGATCGTCGACGTGGCGCTGGATCTGCGCAACAACAAATAA

Upstream 100 bases:

>100_bases
TGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCTCAGTCACTGGCG
CGCAAGGCAGGAGAAGCGGC

Downstream 100 bases:

>100_bases
TGATGACTGCCGAGAACGTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTAAACGCTTTGTTCGGCCT
ACATTTTCTTCGCCTGTAGG

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNDKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSIEVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHA
KIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLEVFI
AAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVDRETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVG
GQEDMIVDVALDLRNNK

Sequences:

>Translated_337_residues
MNDKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSIEVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHA
KIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLEVFI
AAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVDRETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVG
GQEDMIVDVALDLRNNK
>Mature_337_residues
MNDKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSIEVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHA
KIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLEVFI
AAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVDRETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVG
GQEDMIVDVALDLRNNK

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1786548, Length=337, Percent_Identity=98.813056379822, Blast_Score=682, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- ProDom:   PD005364 [H]

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 36467; Mature: 36467

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSIEVAHGDGLQGSSFNYG
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
FGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADVSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGATCIYVVDSGGAMNMSDIRDR
HHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
FRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLEVFI
HHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
AAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVDRETLALGYAGVYSSFLRHCETAAA
EEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
RYGLSAVDILVELGKRRMVGGQEDMIVDVALDLRNNK
HHCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MNDKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSIEVAHGDGLQGSSFNYG
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
FGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADVSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGATCIYVVDSGGAMNMSDIRDR
HHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
FRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLEVFI
HHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
AAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVDRETLALGYAGVYSSFLRHCETAAA
EEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
RYGLSAVDILVELGKRRMVGGQEDMIVDVALDLRNNK
HHCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA