Click here to switch to the map view.

The map label for this gene is hemL

Identifier: 218698575

GI number: 218698575

Start: 184868

End: 186148

Strand: Reverse

Name: hemL

Synonym: ECIAI39_0158

Alternate gene names: 218698575

Gene position: 186148-184868 (Counterclockwise)

Preceding gene: 218698578

Following gene: 218698568

Centisome position: 3.63

GC content: 55.5

Gene sequence:

>1281_bases
ATGAGTAAGTCTGAAAATCTTTACAGCGCAGCGCGCGAGCTGATCCCTGGCGGCGTGAACTCCCCTGTTCGCGCCTTTAC
TGGCGTGGGCGGCACTCCACTGTTTATCGAAAAAGCGGACGGCGCTTATCTGTACGATGTTGATGGCAAAGCCTATATCG
ATTATGTCGGTTCCTGGGGGCCGATGGTGCTGGGCCATAACCATCCGGCGATCCGCAATGCCGTGATTGAAGCCGCCGAG
CGTGGTTTAAGCTTTGGTGCACCAACCGAAATGGAAGTGAAAATGGCGCAACTGGTGACCGAACTGGTCCCGACCATGGA
TATGGTGCGCATGGTGAACTCCGGCACTGAAGCGACGATGAGCGCCATCCGCCTGGCCCGTGGTTTTACCGGTCGCGACA
AAATTATTAAATTTGAAGGTTGTTACCACGGTCACGCTGACTGCCTGCTGGTGAAAGCCGGTTCTGGCGCACTCACGTTA
GGCCAGCCAAATTCGCCGGGCGTTCCGGCAGATTTCGCCAAACATACCTTAACCTGTACTTATAACGATCTGGCCTCTGT
ACGCGCCGCGTTTGAGCAATACCCGCAAGAGATTGCCTGTATTATCGTCGAGCCGGTGGCAGGCAATATGAACTGTGTTC
CGCCGCTGCCAGAGTTCCTGCCAGGTCTGCGTGCGCTGTGCGACGAATTTGGCGCGTTGCTGATCATCGATGAAGTGATG
ACCGGTTTCCGCGTAGCGCTAGCTGGCGCACAGGATTATTACGGCGTAGTGCCAGATTTAACCTGCCTCGGCAAAATCAT
CGGCGGTGGAATGCCGGTAGGCGCATTCGGTGGTCGTCGTGATGTAATGGATGCGCTGGCCCCGACTGGTCCGGTCTATC
AGGCGGGTACGCTTTCCGGTAACCCGATTGCGATGGCGGCCGGTTTCGCCTGTCTGAATGAAGTCGCACAGCCGGGCGTT
CACGAAACGCTGGATGAGCTGACAACACGTCTGGCAGAAGGTCTGCGGGAAGCGGCAGAAGAAGCCGGAATTCCGCTGGT
GGTAAACCACGTTGGCGGCATGTTCGGTATTTTCTTTACCGACGCCGAGTCCGTGACGTGCTATCAGGATGTGATGGCCT
GTGACGTGGAACGCTTTAAGCGTTTCTTCCATATGATGCTGGACGAAGGTGTTTATCTGGCACCGTCAGCGTTTGAAGCG
GGCTTTATGTCTGTGGCGCATAGCATGGAAGATATCAATAACACCATCGATGCTGCACGTCGGGTGTTTGCGAAGTTGTA
A

Upstream 100 bases:

>100_bases
TGCACCAGTACAAGCAGCCTGATGTTTGACGAGTATTTAACTTGTTATGAATAACATAGAATAGCAGCCATTCACTTTTT
CTACGAATCAGGAACCCTCC

Downstream 100 bases:

>100_bases
TGATTTTGCCTCGCCCACCGGGCGAGGCATCCTATTAACGGCTCTGCTTTCTCAACAAATAGATAAAATATGGCGCGCCG
ATAAAGGTCGACAGCAGCCC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAE
RGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
TGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGV
HETLDELTTRLAEGLREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEA
GFMSVAHSMEDINNTIDAARRVFAKL

Sequences:

>Translated_426_residues
MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAE
RGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
TGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGV
HETLDELTTRLAEGLREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEA
GFMSVAHSMEDINNTIDAARRVFAKL
>Mature_425_residues
SKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAER
GLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLG
QPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVMT
GFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVH
ETLDELTTRLAEGLREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAG
FMSVAHSMEDINNTIDAARRVFAKL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=305, Percent_Identity=30.1639344262295, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI13994255, Length=376, Percent_Identity=27.1276595744681, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI284507298, Length=220, Percent_Identity=30.9090909090909, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=99.5305164319249, Blast_Score=866, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=446, Percent_Identity=30.0448430493274, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787560, Length=346, Percent_Identity=32.0809248554913, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1788044, Length=312, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1789759, Length=317, Percent_Identity=31.8611987381703, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI145693181, Length=334, Percent_Identity=29.940119760479, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786991, Length=292, Percent_Identity=27.3972602739726, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71992977, Length=360, Percent_Identity=28.8888888888889, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=299, Percent_Identity=29.0969899665552, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32564660, Length=330, Percent_Identity=24.8484848484848, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=142, Percent_Identity=34.5070422535211, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=340, Percent_Identity=29.1176470588235, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324432, Length=350, Percent_Identity=26.2857142857143, Blast_Score=93, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21357415, Length=314, Percent_Identity=28.343949044586, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356575, Length=340, Percent_Identity=27.6470588235294, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45384; Mature: 45252

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWG
CCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEECCCCC
PMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTLTCT
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHCEEEEE
YNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
HHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEHHHHH
TGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSG
HHHHHHHCCCCHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGFACLNEVAQPGVHETLDELTTRLAEGLREAAEEAGIPLVVNHVGGMFGIFFT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEE
DAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAGFMSVAHSMEDINNTIDAAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RVFAKL
HHHHCC
>Mature Secondary Structure 
SKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWG
CCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEECCCCC
PMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTLTCT
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHCEEEEE
YNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
HHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEHHHHH
TGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSG
HHHHHHHCCCCHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGFACLNEVAQPGVHETLDELTTRLAEGLREAAEEAGIPLVVNHVGGMFGIFFT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEE
DAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAGFMSVAHSMEDINNTIDAAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RVFAKL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA