Click here to switch to the map view.

The map label for this gene is zraS

Identifier: 218697711

GI number: 218697711

Start: 4591785

End: 4593161

Strand: Direct

Name: zraS

Synonym: EC55989_4488

Alternate gene names: 218697711

Gene position: 4591785-4593161 (Clockwise)

Preceding gene: 218697709

Following gene: 218697712

Centisome position: 89.08

GC content: 54.39

Gene sequence:

>1377_bases
ATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGT
AGGGTTGTTTGCGGTGACGGTGATTCGCGATTATGGGCGCGAGACTGCCGCCGCCAGACAAACGCTGCTGGAAAAAGGCA
GTGTACTTATTCGCGCTCTTGAATCCGGCTCGCGCGTCGGCATGGGGATGCGCATGCATCATGCGCAGCAGCAGGCCTTA
CTGGAAGAAATGGCCGGGCAGCCTGGAGTACGTTGGTTTGCGGTCACGGATGAACAAGGAACAATCGTGATGCATAGCAA
CTCCGGCATGGTGGGAAAACAGCTTTATTCCCCGCAGGAAATGCAGCAGTTACATCCGGGAGATGAAGAAGCGTGGCGGC
GGATCGATAGCGCAGACGGCGAGCCTGTTCTGGAAATTTATCGCCAGTTTCAACCGATGTTTGCTGCTGGAATGCACCGG
ATGCGCCATATGCAGCAGTATGCCGCGACACCACAAACAATTTTCATCGCTTTCGACGCCAGTAATATTGTGAGTGCCGA
AGATCGTGAGCAGAGAAACACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCT
GGTATCGCCGCTATCTGCGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTAGTGGCGCTGGGGCAT
CTTGCGGCAGGCGTTGCCCACGAAATCCGTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATACTTTGCCGAGCGCGC
GCCTGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAGGCCGACCGTTTAAACCGCGTGGTAAGCGAGC
TGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTG
GTAGGCCAGGATGCAAACAGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACATTACCGGAAATTCAGGCCGATCC
GGACAGGCTGACTCAGGTCCTGTTGAATCTCTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGA
CGGTCAGCGAAAGCGGCGCGGGCGTGAAAATCAGCGTTACCGACAGCGGTAAGGGAATTGCGGCAGATCAGCTTGAAGCC
ATCTTCACTCCGTACTTCACCACCAAAGCCGAAGGCACCGGATTGGGGCTGGCGGTCGTGCATAATATTGTTGAACAACA
CGGTGGTACAATTCAGGTCGCAAGCCAGGAGGGAAAAGGCTCAACGTTCACCCTCTGGCTTCCGGTCAATATTACGCGTA
AGGACCCACAAGGATGA

Upstream 100 bases:

>100_bases
TCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGG
ATGAGCAGGCAAAGAAGAAG

Downstream 100 bases:

>100_bases
CGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGGCTGG
GGCTATAACGTCGCGCTGGC

Product: sensor protein ZraS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQAL
LEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
MRHMQQYAATPQTIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQL
VGQDANSREIQLRFTANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKGIAADQLEA
IFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVNITRKDPQG

Sequences:

>Translated_458_residues
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQAL
LEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
MRHMQQYAATPQTIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQL
VGQDANSREIQLRFTANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKGIAADQLEA
IFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVNITRKDPQG
>Mature_458_residues
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQAL
LEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
MRHMQQYAATPQTIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQL
VGQDANSREIQLRFTANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKGIAADQLEA
IFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVNITRKDPQG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=465, Percent_Identity=85.1612903225806, Blast_Score=793, Evalue=0.0,
Organism=Escherichia coli, GI1788549, Length=242, Percent_Identity=42.1487603305785, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1790300, Length=239, Percent_Identity=29.2887029288703, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI48994928, Length=246, Percent_Identity=30.8943089430894, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788713, Length=235, Percent_Identity=28.5106382978723, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1789808, Length=218, Percent_Identity=26.605504587156, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=26.0089686098655, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=30.3571428571429, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI87081816, Length=218, Percent_Identity=28.8990825688073, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788393, Length=225, Percent_Identity=27.5555555555556, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790346, Length=219, Percent_Identity=26.027397260274, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786912, Length=221, Percent_Identity=27.6018099547511, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789149, Length=225, Percent_Identity=24.8888888888889, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=25.9090909090909, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI87082128, Length=226, Percent_Identity=24.7787610619469, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787894, Length=219, Percent_Identity=26.9406392694064, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790567, Length=235, Percent_Identity=29.3617021276596, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790861, Length=203, Percent_Identity=28.0788177339901, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50518; Mature: 50518

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS50109 HIS_KIN ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHH
ESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQE
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCHHHHCCHHH
MQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHRMRHMQQYAATPQTIFIAFDA
HHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
SNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
CCCCCCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
THLALQAVDLNTLINHSLQLVGQDANSREIQLRFTANDTLPEIQADPDRLTQVLLNLYLN
HHHHHEEHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
AIQAIGQHGVISVTVSESGAGVKISVTDSGKGIAADQLEAIFTPYFTTKAEGTGLGLAVV
HHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHCCCEECCCCCCCCHHHHH
HNIVEQHGGTIQVASQEGKGSTFTLWLPVNITRKDPQG
HHHHHHCCCEEEEECCCCCCCEEEEEEEEEEECCCCCC
>Mature Secondary Structure
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHH
ESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQE
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCHHHHCCHHH
MQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHRMRHMQQYAATPQTIFIAFDA
HHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
SNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
CCCCCCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
THLALQAVDLNTLINHSLQLVGQDANSREIQLRFTANDTLPEIQADPDRLTQVLLNLYLN
HHHHHEEHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
AIQAIGQHGVISVTVSESGAGVKISVTDSGKGIAADQLEAIFTPYFTTKAEGTGLGLAVV
HHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHCCCEECCCCCCCCHHHHH
HNIVEQHGGTIQVASQEGKGSTFTLWLPVNITRKDPQG
HHHHHHCCCEEEEECCCCCCCEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]