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The map label for this gene is rspA

Identifier: 218697675

GI number: 218697675

Start: 4545620

End: 4546834

Strand: Direct

Name: rspA

Synonym: EC55989_4443

Alternate gene names: 218697675

Gene position: 4545620-4546834 (Clockwise)

Preceding gene: 218697674

Following gene: 218697676

Centisome position: 88.18

GC content: 50.12

Gene sequence:

>1215_bases
ATGAAAATTATTGCTGCAGATGTTTTTGTTACTTGCCCAGGGCGTAATTTCGTTACGCTTAAAATAACCACTGAAAGCGG
ACTTTGTGGATTAGGAGATGCCACGTTAAATGGTCGCGAATTGTCGGTCGCTTCTTATCTGAAAGATCATCTTTGTCCAC
AACTAATTGGCCGCGATGCCAGCCGTATCGAAGATATCTGGCAATTCTTTTATAAAGGTGCTTACTGGCGACGTGGCCCC
GTCACGATGTCAGCCATTTCTGCCATTGATATGGCGTTGTGGGATATCAAAGCTAAAGCGGCAAATATGCCATTGTATCA
ACTGCTTGGTGGTGCTTCTCGTGAAGGCGTGATGGTTTATTGCCACACCACCGGGCGGACGATTGATGAAGTCCTTGAAG
ACTATGCAAAGCACCAGCAGATGGGCTTTAAGGCGATTCGTGTGCAGTGCGGTGTACCGGGTATGCAAACTACTTATGGC
CTGGCGAAGGGCAAAGGCCTGGCGTATGAACCGGCAACCAAAGGCCTGTGGCCAGAAGAGCAGTTGTGGTCTTCAGAAAA
GTATCTCGATTTTACGCCGAAGTTATTTGAAGCGGTGCGTAATAAGTTCGGTTTTAACGAACATCTGTTGCATGACATGC
ACCATCGCCTGACCCCCATCGAAGCGGCGCGCTTTGGGAAGAGCATTGAGGATTACCGCCTGTTCTGGATGGAAGATCCG
ACGCCTGCAGAGAACCAGGAATGTTTTCGTCTGATTCGCCAGCATACCGTGACGCCGATTGCGGTCGGTGAAGTGTTTAA
CAGTATCTGGGACTGCAAGCAGTTAATCGAAGAGCAGCTGATTGACTATATTCGCACGACGATCACCCATGCAGGCGGCA
TTACCGGGATGCGGCGGATTGCCGATTTTGCATCGCTTTATCAGGTACGTACCGGTTCGCATGGCCCTTCAGATTTATCG
CCTGTTTGTCATGCGGCGGCGCTGCATTTTGACCTATGGGTACCAAATTTTGGCGTTCAGGAGTACATGGGCTATTCCGA
ACAAATGCTTGAAGTTTTCCCGCACAGCTGGCGCTTCGAGGAAGGTTATATGCACCCAGGCGATGAACCGGGGCTGGGTA
TTTCTTTCGATGAGAAACTGGCGGCGAAATATCCGTATGACCCGGCGTATCTCCCCGTCGCACGACTGGAAGATGGCACA
TTGTGGAACTGGTAA

Upstream 100 bases:

>100_bases
TAATGTGATCACGGTTGAATAATCGCTCTTTCTCTATTGTATGGTAGTAGGCGTAAAATTAAGTTTGAAGCGTCTATTTA
CAACACAGGAGATTGCAATA

Downstream 100 bases:

>100_bases
TAACGGTTCGCTTAATTATTTTCTGAATAGTCATCAAATCGATTAATTCCGCTCAAGGAGCTAAAAATGGACGTTGATGT
GTCAACCAGTGTTGCGGGTA

Product: putative enolase/dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGP
VTMSAISAIDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRLFWMEDP
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLS
PVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLPVARLEDGT
LWNW

Sequences:

>Translated_404_residues
MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGP
VTMSAISAIDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRLFWMEDP
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLS
PVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLPVARLEDGT
LWNW
>Mature_404_residues
MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGP
VTMSAISAIDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRLFWMEDP
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLS
PVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLPVARLEDGT
LWNW

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=404, Percent_Identity=91.5841584158416, Blast_Score=796, Evalue=0.0,
Organism=Escherichia coli, GI48994953, Length=414, Percent_Identity=29.7101449275362, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 45811; Mature: 45811

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYLKDHLCPQLIGRDA
CEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCH
SRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAANMPLYQLLGGASREGVMVY
HHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEE
CHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYGLAKGKGLAYEPATKGLWPEE
EECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCHH
QLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRLFWMEDP
HHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCC
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTITHAGGITGMRRI
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFE
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEECCCCCHHHHHCHHHHHHHHCCCCCCCC
EGYMHPGDEPGLGISFDEKLAAKYPYDPAYLPVARLEDGTLWNW
CCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYLKDHLCPQLIGRDA
CEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCH
SRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAANMPLYQLLGGASREGVMVY
HHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEE
CHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYGLAKGKGLAYEPATKGLWPEE
EECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCHH
QLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRLFWMEDP
HHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCC
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTITHAGGITGMRRI
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFE
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEECCCCCHHHHHCHHHHHHHHCCCCCCCC
EGYMHPGDEPGLGISFDEKLAAKYPYDPAYLPVARLEDGTLWNW
CCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]