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The map label for this gene is pflD

Identifier: 218697665

GI number: 218697665

Start: 4531139

End: 4533436

Strand: Direct

Name: pflD

Synonym: EC55989_4433

Alternate gene names: 218697665

Gene position: 4531139-4533436 (Clockwise)

Preceding gene: 218697664

Following gene: 218697666

Centisome position: 87.9

GC content: 53.09

Gene sequence:

>2298_bases
ATGACGAATCGTATCTCTCGCCTCAAAACTGCACTGTTTGCCAATACCCGTGAAATCTCGCTGGAGCGGGCGCTGCTTTA
TACCGCCAGCCATCGGCAAACCGAAGGCGAACCGGTGATATTGCGCCGGGCGAAAGCAACAGCGTATATCCTTGAACATG
TTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCTGGGATTATGTCGCCGGAA
ATGGACCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGTGAAGAAGA
TAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAG
ATGAAGTAAAAGCCGCGACCAGCACGCAGATTTTCAGCATCAACCAGACAGATAAAGGCCAGGGGCACATTATTATTGAT
TACCCACGCCTGTTGAATCACGGGCTGGGGGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCA
CTTTTATCAGGCAGCGCTGTTACTGCTGGAAGCCTCGCAGAAACACATTTTGCGTTACGCCGAACTGGCGGAAACGATGG
CGGCAAACTGCACAGATGCCCAGCGTCGCGAAGAGCTGCTGACTATTGCGGAGATCTCCCGCCATAACGCGCAACATAAG
CCGCAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCT
ATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGA
AAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATCGTGCTGTTGCGCTCCACCAGTAGCGCGCGTTATTTCGCC
GGTTTCCCGACCGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCT
TTGCCTTGACGCCTATCAAAGCGTGCAATTACCGCAACCGAACCTCGGCGTGCGCACTAACGCCTTGATCGACACGCCGT
TCCTGATGAAAACCGCCGAAACCATTCGCCTCGGCACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCG
TTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAG
AACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGTATGAAAATGAAGGCAATG
CTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAGGGCAGC
AATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCGTCTTTTATCAGCGATTGTCTGGAAAAAGG
CCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGGATCGGTATCGCCAACCTGAGCGATTCTC
TCCATGCGTTGAAAGGGATGGTTTTTGAGCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTT
GCAACGCCAGAAGGCGAAAAAGTCCGTGCTCGCTTAATTAACCGCTTCGAGAAATACGGTAACGATATCGACGAGGTGGA
TAACATCAGCGCCGAACTGTTACGCCACTACTGCAAAGAAGTGGAAAAATACCAGAACCCGCGCGGCGGTTACTTCACGC
CGGGATCGTATACCGTTTCTGCTCACGTCCCGTTGGGATCGGTGGTTGGCGCGACGCCAGACGGTCGTTTTGCCGGGGAA
CAACTGGCAGACGGCGGCTTGTCACCTATGCTGGGCCAGGACGCACAAGGGCCAACAGCGGTACTGAAGTCAGTCAGTAA
GCTCGATAACACGCTGCTGTCTAACGGTACGTTGCTGAACGTGAAATTCACTCCGGCGACCCTGGAAGGTGAAGCGGGAT
TACGCAAACTGGCCGACTTCTTACGGGCGTTTACCCAGCTTAAGTTGCAGCATATTCAGTTTAACGTGGTGAACGCCGAC
ACGTTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGT
CGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAA

Upstream 100 bases:

>100_bases
CTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCG
CTTTCCCACAGGAGTTCCTC

Downstream 100 bases:

>100_bases
CGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTC
GTACGGTGGTCTTTTTTAAA

Product: putative formate acetyltransferase 2

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 765; Mature: 764

Protein sequence:

>765_residues
MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE
MDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQTSLTQGEDAAFLKELLESLWVKCNDIVLLRSTSSARYFA
GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA
FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLYENEGNAALTYEGLLEQIRAKISHYITLMVEGS
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANF
ATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE
QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL

Sequences:

>Translated_765_residues
MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE
MDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQTSLTQGEDAAFLKELLESLWVKCNDIVLLRSTSSARYFA
GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA
FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLYENEGNAALTYEGLLEQIRAKISHYITLMVEGS
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANF
ATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE
QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
>Mature_764_residues
TNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEM
DPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIIDY
PRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHKP
QTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQTSLTQGEDAAFLKELLESLWVKCNDIVLLRSTSSARYFAG
FPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPAF
LNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLYENEGNAALTYEGLLEQIRAKISHYITLMVEGSN
ICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFA
TPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQ
LADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADT
LREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=765, Percent_Identity=99.6078431372549, Blast_Score=1583, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=807, Percent_Identity=37.546468401487, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI48994926, Length=641, Percent_Identity=25.7410296411856, Blast_Score=153, Evalue=5e-38,
Organism=Escherichia coli, GI1787131, Length=540, Percent_Identity=25.1851851851852, Blast_Score=141, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85926; Mature: 85795

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRD
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHEEEEEC
EELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWE
CCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHH
KRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIIDYPRLLNHGLGELVAQMQQHC
HHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHH
QQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQTSLTQGEDAAFLKELLESL
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
WVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQP
CCCCCCEEEEECCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
NLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPAFLNRGVSLEDARDYSVVGCV
CCCCCCCCEECCCHHHHHHHHHEECCCCCHHHCCCEEEHHHHCCCCCCCCCCCCEEEEEE
ELSIPGRTYGLHDIAMFNLLKVMEICLYENEGNAALTYEGLLEQIRAKISHYITLMVEGS
EEECCCCCCCHHHHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHHEEEEEEECC
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGM
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEECCCHHHHHHHHHH
VFEQQRLSFDELLSVLKANFATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKE
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTA
HHHHCCCCCCEECCCCEEEEECCCCCHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHH
VLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECHH
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
HHHHHHHCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHEEEEEC
EELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFAISEEDKRIYREELFPYWE
CCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHH
KRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIIDYPRLLNHGLGELVAQMQQHC
HHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHH
QQQPENHFYQAALLLLEASQKHILRYAELAETMAANCTDAQRREELLTIAEISRHNAQHK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQTSLTQGEDAAFLKELLESL
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
WVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQP
CCCCCCEEEEECCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
NLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPAFLNRGVSLEDARDYSVVGCV
CCCCCCCCEECCCHHHHHHHHHEECCCCCHHHCCCEEEHHHHCCCCCCCCCCCCEEEEEE
ELSIPGRTYGLHDIAMFNLLKVMEICLYENEGNAALTYEGLLEQIRAKISHYITLMVEGS
EEECCCCCCCHHHHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHHEEEEEEECC
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGM
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEECCCHHHHHHHHHH
VFEQQRLSFDELLSVLKANFATPEGEKVRARLINRFEKYGNDIDEVDNISAELLRHYCKE
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTA
HHHHCCCCCCEECCCCEEEEECCCCCHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHH
VLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECHH
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
HHHHHHHCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]