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The map label for this gene is rffT

Identifier: 218697511

GI number: 218697511

Start: 4357819

End: 4358898

Strand: Direct

Name: rffT

Synonym: EC55989_4265

Alternate gene names: 218697511

Gene position: 4357819-4358898 (Clockwise)

Preceding gene: 218697510

Following gene: 218697512

Centisome position: 84.54

GC content: 52.59

Gene sequence:

>1080_bases
ATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTGCGGTTTTTCAATGACGCGCT
GGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCGCTTT
CTGTGCAATTTTTCCCTGGGAAAAAATCGCTGGCGGAAGCGGTCATCGCGAAAGCAAAAGCTAACCGTCAGCAGCGTTTT
TTCTTCCACGGTCAGTTCAATCCCACACTGTGGCTGGCTCTGCTGAGTGGTGGCATTAAGCCCAGCCAGTTTTTCTGGCA
TATCTGGGGGGCAGACCTGTATGAGCTTTCCAGTGGCTTGAGATATAAGCTTTTTTACCCACTACGTCGCCTGGCGCAAA
AGCGAGTCGGCTGTGTATTTGCCACCCGCGGCGATTTGAGCTTTTTTGCCAAAACGCATCCAAAGGTGCGGGGCGAACTG
CTGTACTTCCCGACGCGGATGGACCCTTCTCTCAATACGATGGCGAACGATCGGCAACGTGAAGGGAAAATGACCATTCT
GGTGGGGAACTCCGGTGACCGCAGCAATGACCATATTGCTGCCTTGTGCGCCGTTCATCAGCAATTTGGCGATACGGTAA
AAGTGGTGGTGCCGATGGGATATCCGCCTAATAACGAAGCGTACATTGAGGAAGTTCGTCAGGCGGGGCTGGAGTTATTC
AGCGAAGAAAATCTGCAAATTTTGAGCGAAAAACTGGAATTTGACGCCTATCTGGCGCTACTTCGTCAGTGCGATCTTGG
TTACTTTATTTTTGCCCGCCAGCAGGGCATTGGTACGCTTTGCTTACTGATTCAGGCGGGCATTCCTTGTGTGCTTAACC
GGGAAAATCCGTTCTGGCAGGATATGACGGAACAGCATTTGCCGGTGCTGTTTACTACCGACGATCTCAACGAGGATATT
GTGCGTGAAGCGCAGCGCCAGTTGGCGTCGGTGGATAAAAACACCATTGCCTTCTTTAGCCCTAACTATCTACAAGGCTG
GCAGCGGGCGTTGGCGATTGCCGCCGGGGAGGTCGCATGA

Upstream 100 bases:

>100_bases
CTGCGCATGGTGCACTGGGCGCGGCGCAGGCATATATGGCAACTTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTT
TTACTCTGGCGTAGGCGGGC

Downstream 100 bases:

>100_bases
GTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTT
CGCCGTGTGCGCTTTAACTT

Product: 4-alpha-L-fucosyltransferase

Products: NA

Alternate protein names: TDP-Fuc4NAc:lipid II Fuc4NAc transferase; Fuc4NAc transferase

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDDGLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRF
FFHGQFNPTLWLALLSGGIKPSQFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVPMGYPPNNEAYIEEVRQAGLELF
SEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQGIGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDI
VREAQRQLASVDKNTIAFFSPNYLQGWQRALAIAAGEVA

Sequences:

>Translated_359_residues
MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDDGLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRF
FFHGQFNPTLWLALLSGGIKPSQFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVPMGYPPNNEAYIEEVRQAGLELF
SEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQGIGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDI
VREAQRQLASVDKNTIAFFSPNYLQGWQRALAIAAGEVA
>Mature_358_residues
TVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDDGLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFF
FHGQFNPTLWLALLSGGIKPSQFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGELL
YFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVPMGYPPNNEAYIEEVRQAGLELFS
EENLQILSEKLEFDAYLALLRQCDLGYFIFARQQGIGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIV
REAQRQLASVDKNTIAFFSPNYLQGWQRALAIAAGEVA

Specific function: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA-Fuc4NAc (Lipid III), the third lipid-linked intermediate involved in ECA synthesis

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 56 family

Homologues:

Organism=Escherichia coli, GI48994971, Length=359, Percent_Identity=98.8857938718663, Blast_Score=737, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WECF_ECO24 (A7ZTZ6)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001465275.1
- ProteinModelPortal:   A7ZTZ6
- STRING:   A7ZTZ6
- EnsemblBacteria:   EBESCT00000021082
- GeneID:   5590275
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_4305
- NMPDR:   fig|331111.3.peg.1938
- eggNOG:   NOG04337
- GeneTree:   EBGT00050000010706
- HOGENOM:   HBG296660
- OMA:   IVPMGYP
- ProtClustDB:   PRK02797
- BioCyc:   ECOL331111:ECE24377A_4305-MONOMER
- HAMAP:   MF_01002
- InterPro:   IPR009993

Pfam domain/function: PF07429 Fuc4NAc_transf

EC number: NA

Molecular weight: Translated: 40490; Mature: 40359

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDDGLSDSCPALSVQFFPG
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEECCC
KKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPSQFFWHIWGADLYELSSGL
HHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCHHHEEEEECCCHHHHHCCCC
RYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGELLYFPTRMDPSLNTMANDRQR
EEEEHHHHHHHHHHHHCEEEEECCCCHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCHHC
EGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVPMGYPPNNEAYIEEVRQAGLELF
CCCEEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
SEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQGIGTLCLLIQAGIPCVLNRENPFWQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCEEECCCCCCHH
DMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKNTIAFFSPNYLQGWQRALAIAAGEVA
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDDGLSDSCPALSVQFFPG
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEECCC
KKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPSQFFWHIWGADLYELSSGL
HHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCHHHEEEEECCCHHHHHCCCC
RYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGELLYFPTRMDPSLNTMANDRQR
EEEEHHHHHHHHHHHHCEEEEECCCCHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCHHC
EGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVPMGYPPNNEAYIEEVRQAGLELF
CCCEEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
SEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQGIGTLCLLIQAGIPCVLNRENPFWQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCEEECCCCCCHH
DMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKNTIAFFSPNYLQGWQRALAIAAGEVA
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA