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The map label for this gene is trmH

Identifier: 218697372

GI number: 218697372

Start: 4199540

End: 4200229

Strand: Direct

Name: trmH

Synonym: EC55989_4117

Alternate gene names: 218697372

Gene position: 4199540-4200229 (Clockwise)

Preceding gene: 218697371

Following gene: 218697373

Centisome position: 81.47

GC content: 55.51

Gene sequence:

>690_bases
ATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGT
CCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTG
GTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGC
GATGCCGTCGCTCATCTCAAAGGCCGGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGA
AATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCGG
ATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAA
GCCCAGCGTCAGCGGCAAAATGCAGGCATGTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTT
GTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCG
AGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAA

Upstream 100 bases:

>100_bases
TCTGACCGCCCGTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCC
GAAACCGAAATTAATGTTTT

Downstream 100 bases:

>100_bases
GTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACTTAGTAACAAACTG
GCGAAAATCAACCTGCATAC

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIG
DAVAHLKGRGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAAG

Sequences:

>Translated_229_residues
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIG
DAVAHLKGRGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAAG
>Mature_229_residues
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIG
DAVAHLKGRGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAAG

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=229, Percent_Identity=99.5633187772926, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1790623, Length=160, Percent_Identity=31.875, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25372; Mature: 25372

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTM
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHEEEECCCCCHHHEEEECCCHHHHHH
ASAAAGSNSWVQVKTHRTIGDAVAHLKGRGMQILATHLSDNAVDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCHHCCCCCCCEEEEEEC
EKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPE
CCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
AEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAAG
HHHHHEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCHHHEEEECCC
>Mature Secondary Structure
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTM
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHEEEECCCCCHHHEEEECCCHHHHHH
ASAAAGSNSWVQVKTHRTIGDAVAHLKGRGMQILATHLSDNAVDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCHHCCCCCCCEEEEEEC
EKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPE
CCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
AEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAAG
HHHHHEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]