Click here to switch to the map view.

The map label for this gene is treF

Identifier: 218697228

GI number: 218697228

Start: 4034701

End: 4036350

Strand: Direct

Name: treF

Synonym: EC55989_3964

Alternate gene names: 218697228

Gene position: 4034701-4036350 (Clockwise)

Preceding gene: 218697223

Following gene: 218697230

Centisome position: 78.27

GC content: 53.64

Gene sequence:

>1650_bases
ATGCTCAATCAGAAAATTCAAAACCCTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCTGGACCCGTA
TGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCCGCCTCTGATGCGCTGA
CGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGAC
TGCGCACCCAAAATGGACCCGCTGGATATTTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAA
GTTTGTTGAAAATCACTTCTGGCTGCCGGAGGTCTACTCCAGAGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGC
ATATCGACCAGCTGTGGCCGGTGCTAACCCGCGAACCACAGGATCACATTCCGTGGTCTTCTCTGCTGGCGCTACCGCAG
TCATATATTGTCCCGGGCGGCCGTTTTAGCGAAACCTACTATTGGGATTCCTATTTCACCATGCTGGGGCTGGCGGAAAG
TGGTCGGGAAGATTTGCTGAAATGCATGGCCGATAACTTCGCCTGGATGATCGAAAACTATGGTCACATCCCCAACGGCA
ACCGCACCTATTATTTGAGCCGATCGCAACCACCGGTTTTTGCGCTGATGGTGGAGTTGTTTGAAGAAGATGGTGTACGC
GGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGTTGATCCCTAATCA
GGCCTATCGCCATGTTGTGCGGATGCCGGACGGATCGCTGCTCAACCGTTATTGGGACGATCGCGACACGCCGCGTGACG
AATCCTGGCTTGAGGACGTTGAAACCGCGAAACATTCTGGTCGCCCGCCCAACGAGGTGTACCGCGATTTACGCGCGGGA
GCGGCCTCAGGTTGGGATTACTCTTCCCGTTGGCTGCGTGATACTGGTCGTCTGGCGAGCATTCGTACCACCCAGTTCAT
CCCCATCGATCTGAATGCCTTCCTGTTTAAACTGGAGAGCGCCATCGCCAACATCTCGGCGCTGAAAGGCGAGAAAGAGA
CAGAAGCGCTGTTCCGCCAGAAGGCCAGTGCCCGTCGCGATGCGGTAAACCGTTACCTCTGGGATGATGAAAACGGCATC
TACCGCGATTACGACTGGCGACGCGAACAACTGGCGCTGTTTTCCGCTGCCGCCATTGTGCCGCTCTATGTCGGCATGGC
GAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTACGCAGCCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGT
ACGAAACCGGTGAACAGTGGGATAAACCCAATGGCTGGGCACCGTTACAATGGATGGCAATTCAGGGATTTAAAATGTAT
GGCGATGACCTTCTGGGTGATGAAATCGCGCGCAGCTGGCTGAAAACGGTGAATCAGTTCTATCTGGAACAGCACAAAAT
GATCGAGAAATACCATATTGCCGATGGTGTTCCCCGCGAAGGCGGCGGTGGCGAGTATCCGTTGCAGGATGGGTTTGGCT
GGACTAACGGTGTGGTACGCCGTTTAATTGGTTTGTACGGCGAACCATAA

Upstream 100 bases:

>100_bases
CGTGATCCACCGCACGCTTTGTCGCCCACCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCCGATAATTAGCGACGA
ATTTCGGAGGTTGGATCCTT

Downstream 100 bases:

>100_bases
TATTTTTACAGCCAGCCGCTAACTTCCTGCTGGCTGTAAAATTATCCTCTTCAGGAGGAGATATTTAACATCATTGCCGC
CTGGGTGCGATTTTTCACTT

Product: trehalase

Products: NA

Alternate protein names: Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSREYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP

Sequences:

>Translated_549_residues
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSREYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP
>Mature_549_residues
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSREYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP

Specific function: Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity [H]

COG id: COG1626

COG function: function code G; Neutral trehalase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 37 family [H]

Homologues:

Organism=Homo sapiens, GI116284412, Length=525, Percent_Identity=34.2857142857143, Blast_Score=255, Evalue=7e-68,
Organism=Escherichia coli, GI1789936, Length=549, Percent_Identity=99.6357012750455, Blast_Score=1127, Evalue=0.0,
Organism=Escherichia coli, GI1787447, Length=487, Percent_Identity=50.9240246406571, Blast_Score=500, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17542196, Length=527, Percent_Identity=31.8785578747628, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI25141398, Length=529, Percent_Identity=31.5689981096408, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17565078, Length=510, Percent_Identity=31.5686274509804, Blast_Score=233, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI25148109, Length=546, Percent_Identity=30.2197802197802, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI71987755, Length=570, Percent_Identity=28.0701754385965, Blast_Score=192, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6320204, Length=429, Percent_Identity=28.6713286713287, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6319473, Length=481, Percent_Identity=28.8981288981289, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24656680, Length=550, Percent_Identity=31.4545454545455, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24656675, Length=550, Percent_Identity=31.4545454545455, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24656661, Length=535, Percent_Identity=31.588785046729, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI17933716, Length=535, Percent_Identity=31.588785046729, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24656670, Length=535, Percent_Identity=31.588785046729, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24656685, Length=486, Percent_Identity=32.0987654320988, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI22024178, Length=533, Percent_Identity=26.078799249531, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI45551104, Length=376, Percent_Identity=26.3297872340426, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28573474, Length=297, Percent_Identity=26.9360269360269, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR001661
- InterPro:   IPR018232 [H]

Pfam domain/function: PF01204 Trehalase [H]

EC number: =3.2.1.28 [H]

Molecular weight: Translated: 63784; Mature: 63784

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00927 TREHALASE_1 ; PS00928 TREHALASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYS
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
REYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
HHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLES
HHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECCEEEEECHHHHHHHHHH
AIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCHHHHEECCHHHH
GDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
RLIGLYGEP
HHHHHCCCC
>Mature Secondary Structure
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYS
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
REYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
HHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLES
HHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECCEEEEECHHHHHHHHHH
AIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCHHHHEECCHHHH
GDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
RLIGLYGEP
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA