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The map label for this gene is silS [H]

Identifier: 218697186

GI number: 218697186

Start: 3987467

End: 3988948

Strand: Direct

Name: silS [H]

Synonym: EC55989_3918

Alternate gene names: 218697186

Gene position: 3987467-3988948 (Clockwise)

Preceding gene: 218697185

Following gene: 218697187

Centisome position: 77.35

GC content: 49.33

Gene sequence:

>1482_bases
ATGCATAGCAAACCTTCCAGACGCCCTTTCTCACTCGCTCTGCGGCTGACCTTTTTTATCAGCCTGTCCACGATACTGGC
TTTTATCGCATTCACCTGGTTTATGCTGCATTCTGTTGAAAATCATTTTGCCGAGCAGGATGTCAGCGATCTTCAACAAA
TCAGCACCACACTGAACCGTATACTGCAGTCCCCGGTGGATCCGGATGATAAAAAAATAAGCAAAATAAAGGAATCAATT
GCCAGCTACCGCAACGTTGCCCTTTTGCTCCTCAATCCCAGGGGGGAAGTGCTCTTTAGCTCAGCTCAGGGGGCGGCACT
ACGCCCGGCAGTGAATTCAGCAGATTTTAGCGAGCACAGCCGCGCACGGGATGTCTTTCTCTGGACGGTGGAGGATCCTG
CGGGACCGATGGATACCGGGTCCGAAATGAAGATGGAAACATACAGGATTATCGCCTCCTCTGGTCAGGCGATATTTCAG
GGCAAACAGCAGAACTATGTCATGCTGACTGGCCTATCCATTAATTTCCATCTCCATTACCTCGATGCGCTGAAAAAGAA
CCTGATTGCGATTGCCGTCGTGATAAGCCTGTTGATTGTTCTGATCATTCGAATCGCTGTCCGTCAGGGGCACCTGCCCC
TTCGTAATGTCAGCAATGCCATTAAAAACATCACCTCCGAGAATCTTGATGCGCGACTGGAACCGACACGCGTTCCCATT
GAGCTGGAGCAACTGGTTATCTCGTTCAATCATATGATTGGAAAGATTGAGGATGTCTTTACCCGCCAGGCCAATTTCTC
TGCCGATATCGCGCATGAGATCAGAACGCCCATCACCAATCTGGTGACGCAGACTGAAATCGCACTGAGTCAGGATCGAA
CACAGAGGGAACTTGAGGATGTCCTCTATTCCAGTCTTGAAGAGTATAACCGGATGACCAAAATGGTCAGCGATATGCTG
TTCCTGGCACAGGCAGATAATAATCAGCTGATACCTGACAGGGTCATGTTTGACCTCAGAGCGGAAGTCATGAAAGTCTT
CGAGTTTTTCGAAGCCTGGGCCGAAGAACGCAATATCACGCTCAAATTTAACGGGATGCCCTGCCTGGTTGAGGGAGATC
CACAAATGTTCAGAAGGGCGATCAATAATCTGTTATCCAATGCCCTGCGTTATACCCCGGAGGGACAGGCAATCACCGTC
TCAATAAGAGAGCAGGAGAGCTTTTTTGACCTTGTGATTGAAAATCCGGGGAAACCAATCCCTGAAGAGCATTTATCAAG
GTTGTTTGACCGTTTTTATCGGGTGGATCCGTCCAGACAACGAAAAGGAGAAGGCAGCGGCATCGGCCTTGCGATTGTGA
AGTCAATCGTGGAAGCACATCACGGAAGAGTGCAGGTGGAATCGGACGTACACTCAACGCGTTTTATCTTATCCGTGCCC
AGACTGGAGAAAATGATTCCGGACACCCAGTGCTGGGAATAA

Upstream 100 bases:

>100_bases
GATGTCGCAGTAAAGCGACTCCGCGCTAAAATAGACAACGATTACGAGACAAAGCTGATCCAGACAGTCCGGGGCGTGGG
CTACATGCTGGAGGTCCCGG

Downstream 100 bases:

>100_bases
AGATTTAAATGACAAAGATGTCATTAGCCTGTCATGCAGCAAACAGAAGCCATTCGATATAATTAGTGCAACTTATCAGG
AAGGCTGGATTGCTTTATCA

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MHSKPSRRPFSLALRLTFFISLSTILAFIAFTWFMLHSVENHFAEQDVSDLQQISTTLNRILQSPVDPDDKKISKIKESI
ASYRNVALLLLNPRGEVLFSSAQGAALRPAVNSADFSEHSRARDVFLWTVEDPAGPMDTGSEMKMETYRIIASSGQAIFQ
GKQQNYVMLTGLSINFHLHYLDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVSNAIKNITSENLDARLEPTRVPI
ELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRTPITNLVTQTEIALSQDRTQRELEDVLYSSLEEYNRMTKMVSDML
FLAQADNNQLIPDRVMFDLRAEVMKVFEFFEAWAEERNITLKFNGMPCLVEGDPQMFRRAINNLLSNALRYTPEGQAITV
SIREQESFFDLVIENPGKPIPEEHLSRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVEAHHGRVQVESDVHSTRFILSVP
RLEKMIPDTQCWE

Sequences:

>Translated_493_residues
MHSKPSRRPFSLALRLTFFISLSTILAFIAFTWFMLHSVENHFAEQDVSDLQQISTTLNRILQSPVDPDDKKISKIKESI
ASYRNVALLLLNPRGEVLFSSAQGAALRPAVNSADFSEHSRARDVFLWTVEDPAGPMDTGSEMKMETYRIIASSGQAIFQ
GKQQNYVMLTGLSINFHLHYLDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVSNAIKNITSENLDARLEPTRVPI
ELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRTPITNLVTQTEIALSQDRTQRELEDVLYSSLEEYNRMTKMVSDML
FLAQADNNQLIPDRVMFDLRAEVMKVFEFFEAWAEERNITLKFNGMPCLVEGDPQMFRRAINNLLSNALRYTPEGQAITV
SIREQESFFDLVIENPGKPIPEEHLSRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVEAHHGRVQVESDVHSTRFILSVP
RLEKMIPDTQCWE
>Mature_493_residues
MHSKPSRRPFSLALRLTFFISLSTILAFIAFTWFMLHSVENHFAEQDVSDLQQISTTLNRILQSPVDPDDKKISKIKESI
ASYRNVALLLLNPRGEVLFSSAQGAALRPAVNSADFSEHSRARDVFLWTVEDPAGPMDTGSEMKMETYRIIASSGQAIFQ
GKQQNYVMLTGLSINFHLHYLDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVSNAIKNITSENLDARLEPTRVPI
ELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRTPITNLVTQTEIALSQDRTQRELEDVLYSSLEEYNRMTKMVSDML
FLAQADNNQLIPDRVMFDLRAEVMKVFEFFEAWAEERNITLKFNGMPCLVEGDPQMFRRAINNLLSNALRYTPEGQAITV
SIREQESFFDLVIENPGKPIPEEHLSRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVEAHHGRVQVESDVHSTRFILSVP
RLEKMIPDTQCWE

Specific function: Component of the sil cation-efflux system that confers resistance to silver. Probable member of a two-component regulatory system silS/silR. May activate silR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=480, Percent_Identity=56.6666666666667, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI1788279, Length=289, Percent_Identity=31.1418685121107, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788393, Length=254, Percent_Identity=30.7086614173228, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1786600, Length=228, Percent_Identity=35.5263157894737, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790346, Length=271, Percent_Identity=29.1512915129151, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789403, Length=342, Percent_Identity=26.6081871345029, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787894, Length=354, Percent_Identity=25.7062146892655, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693157, Length=270, Percent_Identity=23.7037037037037, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790551, Length=282, Percent_Identity=27.3049645390071, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=28.0701754385965, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789149, Length=213, Percent_Identity=27.6995305164319, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790861, Length=428, Percent_Identity=24.2990654205607, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081816, Length=248, Percent_Identity=27.8225806451613, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=25.4545454545455, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789808, Length=243, Percent_Identity=27.9835390946502, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI48994928, Length=225, Percent_Identity=26.2222222222222, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787374, Length=274, Percent_Identity=23.7226277372263, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082128, Length=213, Percent_Identity=25.8215962441315, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56126; Mature: 56126

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSKPSRRPFSLALRLTFFISLSTILAFIAFTWFMLHSVENHFAEQDVSDLQQISTTLNR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ILQSPVDPDDKKISKIKESIASYRNVALLLLNPRGEVLFSSAQGAALRPAVNSADFSEHS
HHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCEECCCCCCCCCHHHC
RARDVFLWTVEDPAGPMDTGSEMKMETYRIIASSGQAIFQGKQQNYVMLTGLSINFHLHY
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCCCCEEEEEEEEEEEEHHH
LDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVSNAIKNITSENLDARLEPTRVPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCH
ELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRTPITNLVTQTEIALSQDRTQRELED
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLYSSLEEYNRMTKMVSDMLFLAQADNNQLIPDRVMFDLRAEVMKVFEFFEAWAEERNIT
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LKFNGMPCLVEGDPQMFRRAINNLLSNALRYTPEGQAITVSIREQESFFDLVIENPGKPI
EEECCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHEECCCCCCC
PEEHLSRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVEAHHGRVQVESDVHSTRFILSVP
CHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHEEECH
RLEKMIPDTQCWE
HHHHHCCCCCCCC
>Mature Secondary Structure
MHSKPSRRPFSLALRLTFFISLSTILAFIAFTWFMLHSVENHFAEQDVSDLQQISTTLNR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ILQSPVDPDDKKISKIKESIASYRNVALLLLNPRGEVLFSSAQGAALRPAVNSADFSEHS
HHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCEECCCCCCCCCHHHC
RARDVFLWTVEDPAGPMDTGSEMKMETYRIIASSGQAIFQGKQQNYVMLTGLSINFHLHY
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCCCCEEEEEEEEEEEEHHH
LDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVSNAIKNITSENLDARLEPTRVPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCH
ELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRTPITNLVTQTEIALSQDRTQRELED
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLYSSLEEYNRMTKMVSDMLFLAQADNNQLIPDRVMFDLRAEVMKVFEFFEAWAEERNIT
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LKFNGMPCLVEGDPQMFRRAINNLLSNALRYTPEGQAITVSIREQESFFDLVIENPGKPI
EEECCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHEECCCCCCC
PEEHLSRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVEAHHGRVQVESDVHSTRFILSVP
CHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHEEECH
RLEKMIPDTQCWE
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9930866 [H]