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The map label for this gene is ygfS

Identifier: 218696481

GI number: 218696481

Start: 3252485

End: 3252973

Strand: Reverse

Name: ygfS

Synonym: EC55989_3172

Alternate gene names: 218696481

Gene position: 3252973-3252485 (Counterclockwise)

Preceding gene: 218696482

Following gene: 218696472

Centisome position: 63.1

GC content: 54.81

Gene sequence:

>489_bases
ATGAAATCGTTAATTATCGTTAATCCGGCTGACTGTATTGGCTGCCGTACTTGTGAAGTAGCCTGTGTGGTCGCTCACCC
TTCAGAACAGGAGTTGAATGCCGATGTCTTTTTACCCCGGCTGAAGGTACAGCGACTGGACAGCATCAGTGCGCCGGTGA
TGTGCCATCAGTGTGAAAACGCCCCTTGTGTTGGCGCTTGCCCCGTGGGGGCGCTGACGATGGGCGAGCAGGTGGTGCAG
ACCAATTCTGCCCGTTGTATTGGCTGTCAGAGTTGCGTCAGTGCGTGCCCGTTTGGAATGATCACCATTCAGTCGTTGCC
GGGGGATACCCGACAACAAATTGTGAAATGCGATCTCTGTGAACAGCGGGAAGAGGGGCCAGCCTGTGTTGAATCTTGCC
CGACACAGGCGCTGCAGTTGCTTACTGAAAGAGAACTCAGGCGAGTCCGCCAGCAGCGTATTGTTGCCAGCGGTGAGAAT
CCTCTGTGA

Upstream 100 bases:

>100_bases
GTGATGCAGTTCATGGCGCGGATCTGGTTGTCACTGCAATGGCCGCAGGAAGGCAGGCGGCGCGCGATATGTTAACTCTG
TTTGATACGAAGGCATCGTG

Downstream 100 bases:

>100_bases
AAGAAATCTCCGCGCTGAAAGGCGCGGAGAATCTATTAGATTGCCCAACCACCCGCGTAAAATGCGACCAGTGCGGCAGT
AATGATCACCGTACCAATAT

Product: putative oxidoreductase, 4Fe-4S ferredoxin-type subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQRLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQ
TNSARCIGCQSCVSACPFGMITIQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
PL

Sequences:

>Translated_162_residues
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQRLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQ
TNSARCIGCQSCVSACPFGMITIQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
PL
>Mature_162_residues
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQRLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQ
TNSARCIGCQSCVSACPFGMITIQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
PL

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI87082179, Length=162, Percent_Identity=100, Blast_Score=322, Evalue=5e-90,
Organism=Escherichia coli, GI1788811, Length=166, Percent_Identity=44.578313253012, Blast_Score=141, Evalue=2e-35,
Organism=Escherichia coli, GI2367245, Length=157, Percent_Identity=45.859872611465, Blast_Score=138, Evalue=2e-34,
Organism=Escherichia coli, GI87082180, Length=163, Percent_Identity=46.0122699386503, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1789067, Length=167, Percent_Identity=40.1197604790419, Blast_Score=130, Evalue=5e-32,
Organism=Escherichia coli, GI87082114, Length=202, Percent_Identity=36.6336633663366, Blast_Score=115, Evalue=1e-27,
Organism=Escherichia coli, GI1789079, Length=184, Percent_Identity=36.4130434782609, Blast_Score=110, Evalue=4e-26,
Organism=Escherichia coli, GI1787122, Length=162, Percent_Identity=31.4814814814815, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1787749, Length=172, Percent_Identity=29.0697674418605, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1787963, Length=163, Percent_Identity=26.9938650306748, Blast_Score=71, Evalue=3e-14,
Organism=Escherichia coli, GI1787872, Length=162, Percent_Identity=30.8641975308642, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1790326, Length=172, Percent_Identity=27.906976744186, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI2367345, Length=138, Percent_Identity=31.8840579710145, Blast_Score=65, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YGFS_ECOLI (Q46819)

Other databases:

- EMBL:   U28375
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   F65072
- RefSeq:   AP_003445.1
- RefSeq:   NP_417362.4
- ProteinModelPortal:   Q46819
- SMR:   Q46819
- STRING:   Q46819
- EnsemblBacteria:   EBESCT00000002789
- EnsemblBacteria:   EBESCT00000015536
- GeneID:   949016
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5468
- KEGG:   eco:b2886
- EchoBASE:   EB2880
- EcoGene:   EG13069
- eggNOG:   COG1142
- GeneTree:   EBGT00050000009106
- HOGENOM:   HBG726839
- OMA:   KERCIAC
- ProtClustDB:   CLSK880521
- BioCyc:   EcoCyc:G7505-MONOMER
- Genevestigator:   Q46819
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900

Pfam domain/function: PF00037 Fer4

EC number: NA

Molecular weight: Translated: 17514; Mature: 17514

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
12.3 %Cys+Met (Translated Protein)
9.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
12.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQRLDSISAPVMCHQCEN
CCCEEEECCHHHCCCCCEEEEEEEECCCCHHCCCHHCCHHHHHHHHHCCCCCEEEECCCC
APCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMITIQSLPGDTRQQIVKCDLC
CCCCCCCCCCHHHHHHHHHHCCCCCEECHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
EQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGENPL
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQRLDSISAPVMCHQCEN
CCCEEEECCHHHCCCCCEEEEEEEECCCCHHCCCHHCCHHHHHHHHHCCCCCEEEECCCC
APCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMITIQSLPGDTRQQIVKCDLC
CCCCCCCCCCHHHHHHHHHHCCCCCEECHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
EQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGENPL
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503