Click here to switch to the map view.

The map label for this gene is ygaF

Identifier: 218696257

GI number: 218696257

Start: 3000598

End: 3001866

Strand: Direct

Name: ygaF

Synonym: EC55989_2928

Alternate gene names: 218696257

Gene position: 3000598-3001866 (Clockwise)

Preceding gene: 218696256

Following gene: 218696258

Centisome position: 58.21

GC content: 57.84

Gene sequence:

>1269_bases
ATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTTTATCCGGATGC
CCGCATTGCGTTGCTGGAAAAAGAGTCCGGCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGG
TCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAC
GACATTCGCTATGACAACTGCGGCAAGATGCTGGTCGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCGTTGTGGGA
ACGCACGGCGGCGAACGGTATCGAGCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAACCGAATATCACCGGGC
TCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGAAGTAACGGCGGCGATGGCAAAAATTTTCCAGGCC
AGAGGCGGCGAGATTATCTATAACGCCGAAGTCAGCGGCCTTAGTGAGCATAAAAGCGGCGTGGTGATACGTACCCGTCA
GGGCGGCGACTATGAAGCATCGACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGCCTCG
AACCGGGCTTTATCATCTGCCCGTTCCGCGGCGAGTATTTCCGCCTGGCGCCGGAGCATAACCAGATTGTTAACCATCTG
ATTTACCCCATTCCCGACCCGGCAATGCCGTTTTTGGGCGTGCATCTCACCCGAATGATCGATGGCAGCGTCACCGTCGG
GCCAAACGCGGTGCTGGCTTTCAAACGCGAAGGCTATCGCAAGCGCGACTTCTCATTTAGCGACACGCTGGAGATTTTGG
GCTCGTCGGGGATTCGCCGGGTGCTGCAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGC
GGCTATCTGCGCCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGC
GCAGGCAGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCACGCACGATCCACACCTGCAATG
CGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAG
AGTAACCCCGGACGCACGCTGCGTGCGGCACGTAGTGTGGATGCCTTACACGCCGCATTTAATCAATAA

Upstream 100 bases:

>100_bases
CACCCGGATCTGCGCCGTGAACTGATGCGTCAGCGTGGCTATTTCGCTTACGCCACTCACCACTACCAGACGCATCAGTA
AGCGCAAAGGAATTGAGCGG

Downstream 100 bases:

>100_bases
CCTTTGAAAACAGGACGTAACAATGAAACTTAACGCCAGTAACTTATTCCGCCAGCAGGCATTGATTAACGGGGAATGGC
TGGACGCCAACAATGGCGAG

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQN
DIRYDNCGKMLVATSDLEMERMRALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
RGGEIIYNAEVSGLSEHKSGVVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKS
GYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQ
SNPGRTLRAARSVDALHAAFNQ

Sequences:

>Translated_422_residues
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQN
DIRYDNCGKMLVATSDLEMERMRALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
RGGEIIYNAEVSGLSEHKSGVVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKS
GYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQ
SNPGRTLRAARSVDALHAAFNQ
>Mature_422_residues
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQN
DIRYDNCGKMLVATSDLEMERMRALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
RGGEIIYNAEVSGLSEHKSGVVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKS
GYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQ
SNPGRTLRAARSVDALHAAFNQ

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=398, Percent_Identity=41.2060301507538, Blast_Score=318, Evalue=7e-87,
Organism=Escherichia coli, GI87082148, Length=422, Percent_Identity=98.1042654028436, Blast_Score=862, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17565764, Length=401, Percent_Identity=42.643391521197, Blast_Score=330, Evalue=6e-91,
Organism=Drosophila melanogaster, GI24585081, Length=407, Percent_Identity=39.3120393120393, Blast_Score=279, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 46071; Mature: 46071

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPGS
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCEEEEEEEECCCC
LKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERMRALWERTAANGIEREWLN
CEEEEEEECCCHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHCCC
AEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSGLSEHKSG
HHHHHHCCCCCEECCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
VVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHL
EEEEECCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHC
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRR
CCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHH
VLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEHHE
LFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAF
EEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
NQ
CC
>Mature Secondary Structure
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPGS
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCEEEEEEEECCCC
LKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERMRALWERTAANGIEREWLN
CEEEEEEECCCHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHCCC
AEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSGLSEHKSG
HHHHHHCCCCCEECCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
VVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHL
EEEEECCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHC
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRR
CCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHH
VLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEHHE
LFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAF
EEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
NQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]