Click here to switch to the map view.

The map label for this gene is hcaT

Identifier: 218696163

GI number: 218696163

Start: 2890060

End: 2891199

Strand: Reverse

Name: hcaT

Synonym: EC55989_2822

Alternate gene names: 218696163

Gene position: 2891199-2890060 (Counterclockwise)

Preceding gene: 218696164

Following gene: 218696159

Centisome position: 56.09

GC content: 56.58

Gene sequence:

>1140_bases
ATGGTTTTGCAATCCACGCGCTGGTTGGCGCTCGGCTATTTCACATACTTTTTTAGTTACGGCATTTTTCTACCTTTCTG
GAGCGTCTGGCTTAAAGGGATTGGTTTAACGCCAGAAACCATCGGCCTGTTATTGGGGGCAGGTCTGGTTGCCCGTTTTC
TCGGGAGTTTGCTCATCGCGCCCCGCGTCAGCGATCCTTCCCGCCTGATTTCCGCCTTGCGCGTGCTGGCACTGCTGACA
CTTCTCTTTGCTGTCGCCTTCTGGGCGGGGGCGCACGTAGCGTGGCTGATGCTGGTGATGATTGGCTTTAACCTCTTTTT
CTCACCGCTGGTACCGTTGACCGATGCACTGGCGAATACGTGGCAAAAGCAGTTCCCGCTTGATTACGGCAAAGTGCGAC
TGTGGGGCTCGGTGGCGTTTGTCATTGGCTCGGCGCTGACGGGCAAACTGGTCACTATGTTTGATTATCGGGTGATCCTC
GCGCTGTTGACGTTGGGCGTGGCATCCATGCTGCTCGGCTTTCTCATCCGTCCGACGATTCAGCCACAAGGGGCAAGCCG
CCAGCAGGAGAGCACCGGTTGGTCAGCGTGGTTGGCGCTGGTTCGCCAGAACTGGCGCTTTCTGGCCTGCGTTTGTTTAT
TGCAGGGGGCACATGCGGCCTATTACGGTTTTAGCGCCATTTACTGGCAGGCAGCTGGCTACTCGGCCTCGGCGGTGGGG
TATTTGTGGTCGCTGGGCGTGGTGGCGGAAGTCATTATCTTTGCGCTGAGTAATAAACTTTTCCGCCGTTGTAGTGCACG
CGATATGCTGTTGATCTCGGCGATTTGCGGCGTAGTGCGCTGGGGCATTATGGGAGCAACTACGGCGTTGCCGTGGTTGA
TAGTGGTGCAAATTCTGCATTGCGGCACCTTCACGGTCTGCCACCTGGCCGCCATGCGTTATATTGCTGCTCGCCAGGGT
AGCGAAGTCATCCGTTTACAGGCGGTTTACTCTGCCGTCGCGATGGGCGGCAGTATCGCTATCATGACCGTTTTCGCCGG
TTTCCTGTATCAATATCTGGGCCACGGCGTGTTCTGGGTAATGGCGCTGGTGGCGCTTCCGGCAATGTTTTTGCGCCCGA
AAGTTGTTCCCTCATGCTGA

Upstream 100 bases:

>100_bases
GCGTTACAGACATCGGGAAGCAGGCTCGAATATTCTTATTTTTCAACTTGCACCAACATTCGATTAACCCCAGACTAACG
CCTCCTGACGGGAGGGACTC

Downstream 100 bases:

>100_bases
TTCGAGCATTTTGCGGATCTGCTCCTGTTGATGTGTTGTCAGCGTCAGGTCCGTATAGATCAGCGGTGGTGAAAAGAGCG
GTAATGGCATGGTATAAGGC

Product: putative 3-phenylpropionic acid transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLLLGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLT
LLFAVAFWAGAHVAWLMLVMIGFNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAHAAYYGFSAIYWQAAGYSASAVG
YLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAICGVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQG
SEVIRLQAVYSAVAMGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC

Sequences:

>Translated_379_residues
MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLLLGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLT
LLFAVAFWAGAHVAWLMLVMIGFNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAHAAYYGFSAIYWQAAGYSASAVG
YLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAICGVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQG
SEVIRLQAVYSAVAMGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC
>Mature_379_residues
MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLLLGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLT
LLFAVAFWAGAHVAWLMLVMIGFNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAHAAYYGFSAIYWQAAGYSASAVG
YLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAICGVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQG
SEVIRLQAVYSAVAMGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily

Homologues:

Organism=Escherichia coli, GI1788886, Length=379, Percent_Identity=100, Blast_Score=748, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HCAT_ECOLI (Q47142)

Other databases:

- EMBL:   Z37966
- EMBL:   Y11071
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   G65030
- PIR:   S49295
- RefSeq:   AP_003122.1
- RefSeq:   NP_417031.1
- ProteinModelPortal:   Q47142
- STRING:   Q47142
- EnsemblBacteria:   EBESCT00000002826
- EnsemblBacteria:   EBESCT00000016936
- GeneID:   947007
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2520
- KEGG:   eco:b2536
- EchoBASE:   EB3227
- EcoGene:   EG13454
- eggNOG:   COG0477
- GeneTree:   EBGT00050000008944
- HOGENOM:   HBG551193
- OMA:   HGVTFAM
- ProtClustDB:   PRK11128
- BioCyc:   EcoCyc:HCAT-MONOMER
- Genevestigator:   Q47142
- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276
- TIGRFAMs:   TIGR00902

Pfam domain/function: PF01306 LacY_symp; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 41593; Mature: 41593

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b595adc)-; HASH(0x1ad3ae98)-; HASH(0x1bb65c28)-; HASH(0x1b6a9b54)-; HASH(0x1af780b8)-; HASH(0x1b7620a8)-; HASH(0x1b8d8d94)-; HASH(0x4097f404)-; HASH(0x1b6c3cf8)-; HASH(0x1ba03b88)-; HASH(0x1ba45ea8)-; HASH(0x1b3ad2ac)-;

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLLLGAGLVARFLGSLLIA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIGFNLFFSPLVPLTDALANT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVILALLTLGVASMLLGFLIRPTI
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAHAAYYGFSAIYWQAAGYSASAVG
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHH
YLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAICGVVRWGIMGATTALPWLIVVQILH
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVAMGGSIAIMTVFAGFLYQYLGHGVFWV
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
MALVALPAMFLRPKVVPSC
HHHHHHHHHHHCCCCCCCH
>Mature Secondary Structure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HHHHHHHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503