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The map label for this gene is cysU

Identifier: 218696059

GI number: 218696059

Start: 2771748

End: 2772581

Strand: Reverse

Name: cysU

Synonym: EC55989_2713

Alternate gene names: 218696059

Gene position: 2772581-2771748 (Counterclockwise)

Preceding gene: 218696060

Following gene: 218696058

Centisome position: 53.79

GC content: 56.0

Gene sequence:

>834_bases
ATGTTTGCAGTTTCATCAAGACGCGTGCTGCCGGGCTTTACCTTAAGCCTCGGCACCAGTCTGCTGTTTGTGTGCCTGAT
TTTGCTGCTGCCGCTCTCCGCGCTGGTGATGCAGCTGGCTCAGATGAGCTGGGCGCAGTACTGGGAGGTGATCACCAACC
CACAAGTGGTCGCGGCCTACAAAGTGACGCTGCTGTCGGCGTTTGTGGCATCGATTTTTAACGGCGTTTTCGGTCTGCTG
ATGGCGTGGATTTTAACACGTTATCGCTTTCCGGGCCGCACGCTGCTTGATGCGCTGATGGATTTACCCTTTGCGCTGCC
AACGGCAGTCGCCGGTTTAACGCTGGCCTCGCTCTTTTCCGTAAACGGTTTTTACGGTGAATGGCTGGCGAAGTTTGATA
TCAAAGTCACCTATACATGGCTGGGGATTGCGGTGGCGATGGCCTTTACCAGCATTCCGTTTGTGGTGCGTACCGTGCAG
CCGGTGCTGGAAGAGTTAGGCCCGGAATATGAAGAAGCGGCGGAAACGCTTGGTGCAACGCGCTGGCAGAGTTTCTGCAA
AGTGGTGCTGCCGGAGCTTTCTCCGGCGCTGGTGGCGGGCGTGGCGCTGTCGTTTACCCGTAGTCTTGGTGAATTTGGCG
CGGTGATTTTTATCGCCGGGAACATCGCCTGGAAGACGGAAGTGACGTCGCTGATGATTTTTGTGCGCTTGCAGGAGTTT
GATTACCCGGCGGCGAGCGCGATTGCTTCGGTGATCCTCGCGGCATCCCTGCTGCTGCTGTTCTCAATTAACACTCTGCA
AAGTCGCTTTGGTCGGCGTGTGGTAGGTCATTAA

Upstream 100 bases:

>100_bases
TCCGCGTGGAAGACAAATTTGGCTCCTGGCCGGAAGTGATGAAAACCCACTTCACCAGCGGCGGCGAGTTAGACAAGCTG
TTAGCGGCGGGGCGTAACTG

Downstream 100 bases:

>100_bases
TGGCGGAAGTTACCCAATTGAAGCGTTATGACGCGCGCCCGATTAACTGGGGCAAATGGTTTCTAATTGGCATCGGGATG
CTGGTTTCAGCGTTCATCCT

Product: sulfate/thiosulfate transporter subunit

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSWAQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLL
MAWILTRYRFPGRTLLDALMDLPFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
DYPAASAIASVILAASLLLLFSINTLQSRFGRRVVGH

Sequences:

>Translated_277_residues
MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSWAQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLL
MAWILTRYRFPGRTLLDALMDLPFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
DYPAASAIASVILAASLLLLFSINTLQSRFGRRVVGH
>Mature_277_residues
MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSWAQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLL
MAWILTRYRFPGRTLLDALMDLPFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
DYPAASAIASVILAASLLLLFSINTLQSRFGRRVVGH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1788764, Length=277, Percent_Identity=100, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI87082099, Length=250, Percent_Identity=37.2, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1786980, Length=232, Percent_Identity=34.4827586206897, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787369, Length=207, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787368, Length=215, Percent_Identity=28.3720930232558, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYST_ECOLI (P16701)

Other databases:

- EMBL:   M32101
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A35402
- RefSeq:   AP_003018.1
- RefSeq:   NP_416919.1
- ProteinModelPortal:   P16701
- SMR:   P16701
- STRING:   P16701
- EnsemblBacteria:   EBESCT00000001200
- EnsemblBacteria:   EBESCT00000014815
- GeneID:   946882
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2417
- KEGG:   eco:b2424
- EchoBASE:   EB0194
- EcoGene:   EG10197
- eggNOG:   COG0555
- GeneTree:   EBGT00050000008926
- HOGENOM:   HBG685300
- OMA:   KVSYTWL
- ProtClustDB:   PRK10971
- BioCyc:   EcoCyc:CYST-MONOMER
- Genevestigator:   P16701
- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667
- TIGRFAMs:   TIGR00969
- TIGRFAMs:   TIGR02139

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 30292; Mature: 30292

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b79edb0)-; HASH(0x1b86ee48)-; HASH(0x1ad5554c)-; HASH(0x1b8821b4)-; HASH(0x1b89969c)-; HASH(0x1b6925b4)-; HASH(0x16c56b2c)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSWAQYWEVITNPQVVAAY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
KVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDLPFALPTAVAGLTLASLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQPVLEELGPEYEEAAETLGAT
CCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHC
DYPAASAIASVILAASLLLLFSINTLQSRFGRRVVGH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCH
>Mature Secondary Structure
MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSWAQYWEVITNPQVVAAY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
KVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDLPFALPTAVAGLTLASLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQPVLEELGPEYEEAAETLGAT
CCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHC
DYPAASAIASVILAASLLLLFSINTLQSRFGRRVVGH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959