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The map label for this gene is xapR

Identifier: 218696040

GI number: 218696040

Start: 2752622

End: 2753506

Strand: Reverse

Name: xapR

Synonym: EC55989_2694

Alternate gene names: 218696040

Gene position: 2753506-2752622 (Counterclockwise)

Preceding gene: 218696041

Following gene: 218696039

Centisome position: 53.42

GC content: 50.4

Gene sequence:

>885_bases
ATGGAACGCGTATACAGAACAGATCTTAAGTTGCTCCGTTATTTTCTTGCCGTAGCGGAAGAGTTGCATTTTGGCCGCGC
AGCAGCGCGTTTAAATATGTCTCAGCCTCCGCTCAGTATTCATATTAAAGAGCTGGAAAATCAACTCGGCACGCAGCTTT
ATATTCGCCATTCGCGCAGCGTCGTACTGACACACGCGGGCAAAATCTTGATGGAAGAATCGCGTCGATTGCTGGTGAAT
GCTAATAATGTATTGGCTCGGGTTGAACAAATAGGTCGGGGAGAAGCAGGGCGGATTGAACTCGGCGTAGTGGGAACGGC
TATGTGGGGACGGATGCGCCCGGTTATGCGGCGATTCCTCAGGGAAAATCCTAACGTTGAAGTTCTTTTTCGCGAAAAGA
TGCCCGCGATGCAAATGGCCCTGCTGGAACGACGTGAACTCGATGCCGGGATCTGGCGAATGGCGACAGAACCACCGACA
GGTTTTACCAGCTTACGGTTGCATGAATCGGCGTTTCTGGTGGCGATGCCTGAAGAGCATCATCTCTCATCATTTTCCAC
CGTTCCGCTGGAAGCGCTACGTGACGAGTATTTTGTTACAATGCCGCCCGTGTACACTGACTGGGATTTTTTGCAGCGAG
TTTGCCAGCAGGTGGGATTTTCACCGGTTGTTATTCGCGAAGTTAATGAACCGCAAACGGTACTCGCCATGGTCAGTATG
GGTATTGGTATCACATTGATAGCGGACAGCTACGCACAAATGAACTGGCCAGGTGTCATTTTCCGTCCGCTCAAACAGCG
CATCCCTGCAGATTTATATATTGTTTATGAAACACAGCAGGTGACGCCCGCGATGGTTAAGCTGTTGGCTGCATTGACAC
AGTAG

Upstream 100 bases:

>100_bases
ATATTATCCATTGTGTTTATGTATGTCGGATATCTGGTGGTGAAATACTTTATGCCATGATAATTTAATACGATGTATTT
ATTATATGGAGCACTTAATT

Downstream 100 bases:

>100_bases
GTGAGGAGACAGGAATTGCCGGAAATAGTCGTCTGCTAAGTGAAGCGCATCAGGCAATTTCGCAAATCGCAGATAGCAAA
AAAGCGCCTTTAGGGCGCTT

Product: DNA-binding transcriptional activator XapR

Products: NA

Alternate protein names: Xanthosine operon regulatory protein [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKELENQLGTQLYIRHSRSVVLTHAGKILMEESRRLLVN
ANNVLARVEQIGRGEAGRIELGVVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVGFSPVVIREVNEPQTVLAMVSM
GIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVYETQQVTPAMVKLLAALTQ

Sequences:

>Translated_294_residues
MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKELENQLGTQLYIRHSRSVVLTHAGKILMEESRRLLVN
ANNVLARVEQIGRGEAGRIELGVVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVGFSPVVIREVNEPQTVLAMVSM
GIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVYETQQVTPAMVKLLAALTQ
>Mature_294_residues
MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKELENQLGTQLYIRHSRSVVLTHAGKILMEESRRLLVN
ANNVLARVEQIGRGEAGRIELGVVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVGFSPVVIREVNEPQTVLAMVSM
GIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVYETQQVTPAMVKLLAALTQ

Specific function: Positive regulator required for the expression of xapA and xapB. Binds to the inducer xanthosine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI2367136, Length=294, Percent_Identity=98.9795918367347, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1787879, Length=257, Percent_Identity=37.7431906614786, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=29.020979020979, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI157672245, Length=227, Percent_Identity=29.9559471365639, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790399, Length=240, Percent_Identity=27.5, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788748, Length=242, Percent_Identity=27.6859504132231, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790208, Length=249, Percent_Identity=26.5060240963855, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693105, Length=244, Percent_Identity=27.0491803278689, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787589, Length=123, Percent_Identity=34.9593495934959, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788297, Length=255, Percent_Identity=27.0588235294118, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=24.5901639344262, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787601, Length=248, Percent_Identity=27.0161290322581, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI48994962, Length=178, Percent_Identity=29.7752808988764, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081904, Length=151, Percent_Identity=31.7880794701987, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33644; Mature: 33644

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKELENQLGTQLYIRHSRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCEEEEEECCE
VVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELGVVGTAMWGRMRPVMRRFL
EEEECCCHHHHHCCCEEEEECHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHH
RENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPTGFTSLRLHESAFLVAMPEEH
HCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHEECCCCCCCCEEEEEECCEEEEECCCHH
HLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVGFSPVVIREVNEPQTVLAMVSM
CCCCCCCCCHHHHCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
GIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVYETQQVTPAMVKLLAALTQ
CCCEEEEECCHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKELENQLGTQLYIRHSRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCEEEEEECCE
VVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELGVVGTAMWGRMRPVMRRFL
EEEECCCHHHHHCCCEEEEECHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHH
RENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPTGFTSLRLHESAFLVAMPEEH
HCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHEECCCCCCCCEEEEEECCEEEEECCCHH
HLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVGFSPVVIREVNEPQTVLAMVSM
CCCCCCCCCHHHHCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
GIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVYETQQVTPAMVKLLAALTQ
CCCEEEEECCHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2201776; 9205837; 9278503; 7559336 [H]