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The map label for this gene is cobU

Identifier: 218695602

GI number: 218695602

Start: 2279884

End: 2280429

Strand: Reverse

Name: cobU

Synonym: EC55989_2227

Alternate gene names: 218695602

Gene position: 2280429-2279884 (Counterclockwise)

Preceding gene: 218695603

Following gene: 218695601

Centisome position: 44.24

GC content: 50.92

Gene sequence:

>546_bases
ATGATGATTTTGGTGACGGGGGGCGCACGGAGCGGGAAGAGTCGCCACGCAGAGGCGCTTATTGGGGACTCTTCACAGGT
TCTGTATATCGCTACCTCGCAAATCCTTGATGATGAGATGGCTGCACGGATAGAACATCATCGGCAAGGCCGCCCGGAGC
ACTGGCGCACAGTGGAGCGCTGGCAACATCTTGATGAATTAATTCATGCAGACATTAACCCGAATGAGGTTGTGTTGCTG
GAATGCGTTACCACAATGGTGACTAATCTGTTGTTTGATTATGGCGGCGATAAAGACCCTGATGAATGGGATTATCAGGC
GATGGAACAGGCGATTAATGCTGAGATTCAGTCGTTGATTGCTGCCTGCCAACGTTGCCCCGCAAAGGTTGTATTAGTGA
CTAACGAAGTGGGAATGGGGATTGTGCCGGAGAGTCGTCTGGCACGACATTTTCGTGATATTGCCGGGCGGGTAAATCAG
CAGCTGGCCGCTGCGGCAAATGAAGTATGGCTGGTGGTTTCGGGTATTGGAGTAAAAATCAAATGA

Upstream 100 bases:

>100_bases
CGCGCAGTATTGATCGTGTAAATGTTTTGCGGTCAGCAATGGTGGGGGATCCATTGTTCTGTTCAATGAATACCATCATA
CAATTGAATCAGGAGTCGGT

Downstream 100 bases:

>100_bases
GTAAATTATTTTGGGCGATGCTCTCATTTATTACGCGTCTGCCTGTACCGCGTCGCTGGTCCCAGGGACTGGATTTCGAG
CATTATTCTCGCGGTATTAT

Product: adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase

Products: NA

Alternate protein names: Adenosylcobinamide kinase; Adenosylcobinamide-phosphate guanylyltransferase

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARIEHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLL
ECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
QLAAAANEVWLVVSGIGVKIK

Sequences:

>Translated_181_residues
MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARIEHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLL
ECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
QLAAAANEVWLVVSGIGVKIK
>Mature_181_residues
MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARIEHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLL
ECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
QLAAAANEVWLVVSGIGVKIK

Specific function: ATP-dependent phosphorylation of adenosylcobinamide and adds GMP to adenosylcobinamide phosphate

COG id: COG2087

COG function: function code H; Adenosyl cobinamide kinase/adenosyl cobinamide phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788302, Length=181, Percent_Identity=100, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBU_ECO57 (P0AE77)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85824
- PIR:   D90977
- RefSeq:   NP_288500.1
- RefSeq:   NP_310815.1
- ProteinModelPortal:   P0AE77
- SMR:   P0AE77
- EnsemblBacteria:   EBESCT00000026095
- EnsemblBacteria:   EBESCT00000059540
- GeneID:   912633
- GeneID:   962053
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3153
- KEGG:   ecs:ECs2788
- GeneTree:   EBGT00050000011433
- HOGENOM:   HBG635321
- OMA:   TLWLTNH
- ProtClustDB:   PRK05800
- BioCyc:   ECOL83334:ECS2788-MONOMER
- InterPro:   IPR003203
- PIRSF:   PIRSF006135

Pfam domain/function: PF02283 CobU

EC number: =2.7.1.156; =2.7.7.62

Molecular weight: Translated: 20164; Mature: 20164

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: ACT_SITE 47-47 BINDING 59-59 BINDING 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARIEHHRQGRPEHWRTVER
CEEEEECCCCCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
WQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLI
HHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
AACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKI
HHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEE
K
C
>Mature Secondary Structure
MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARIEHHRQGRPEHWRTVER
CEEEEECCCCCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
WQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLI
HHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
AACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKI
HHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEE
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796