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The map label for this gene is manZ

Identifier: 218695381

GI number: 218695381

Start: 2054293

End: 2055153

Strand: Direct

Name: manZ

Synonym: EC55989_1992

Alternate gene names: 218695381

Gene position: 2054293-2055153 (Clockwise)

Preceding gene: 218695380

Following gene: 218695382

Centisome position: 39.85

GC content: 52.85

Gene sequence:

>861_bases
GTGAGCGAAATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCG
TTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTC
GTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTC
GTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCAGAGATCGACGACGGTGCTAT
CAACGGTATCAAAGTCGGTTTGATGGGGCCGCTGGCTGGTGTAGGCGACCCGATCTTCTGGGGAACCGTACGTCCGGTAT
TTGCAGCACTGGGTGCCGGTATCGCGATGAGCGGCAGCCTGTTAGGTCCGCTGCTGTTCTTCATCCTGTTTAACCTGGTG
CGTCTGGCAACCCGTTACTACGGCGTAGCGTATGGTTACTCCAAAGGTATCGATATCGTTAAAGATATGGGTGGTGGCTT
CCTGCAAAAACTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCA
ACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAG
TTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGAT
CATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAA

Upstream 100 bases:

>100_bases
AGTGCTCTACATCCAACTTAGCCCGAAATACAACCGCGTAGCCGGTGCGCCTGCTCAGGCAGCTGGTAACAACGATCTCG
ATAACGAACTGGACTAACAG

Downstream 100 bases:

>100_bases
GACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGAT
TCTTTTCATCGCCGCACTCC

Product: PTS system mannose-specific transporter subunit IID

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSEMVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPENNEARKQAIRRHLEFFNTQPF
VAAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLV
RLATRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSRITDQTGKEHVTTVQTILDQ
LMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLLGL

Sequences:

>Translated_286_residues
MSEMVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPENNEARKQAIRRHLEFFNTQPF
VAAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLV
RLATRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSRITDQTGKEHVTTVQTILDQ
LMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLLGL
>Mature_285_residues
SEMVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFV
AAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVR
LATRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSRITDQTGKEHVTTVQTILDQL
MPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLLGL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain

Homologues:

Organism=Escherichia coli, GI1788122, Length=286, Percent_Identity=100, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1789529, Length=275, Percent_Identity=34.5454545454545, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTND_ECO57 (P69807)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A98945
- PIR:   D85793
- RefSeq:   NP_288255.1
- RefSeq:   NP_310556.3
- ProteinModelPortal:   P69807
- EnsemblBacteria:   EBESCT00000025527
- EnsemblBacteria:   EBESCT00000056670
- GeneID:   914171
- GeneID:   961792
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2862
- KEGG:   ecs:ECs2529
- GeneTree:   EBGT00050000009643
- HOGENOM:   HBG421686
- OMA:   VTMAMEE
- ProtClustDB:   PRK11103
- BioCyc:   ECOL83334:ECS2529-MONOMER
- InterPro:   IPR004704
- InterPro:   IPR018405
- TIGRFAMs:   TIGR00828

Pfam domain/function: PF03613 EIID-AGA

EC number: NA

Molecular weight: Translated: 31303; Mature: 31172

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1a16d694)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPEN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
NEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAG
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
VGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLATRYYGVAYGYSKGIDIV
CCCCCHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHH
KDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSRITDQTGKEHVTTVQTILDQ
HHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
LMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLLGL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
SEMVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPEN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
NEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAG
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
VGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLATRYYGVAYGYSKGIDIV
CCCCCHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHH
KDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSRITDQTGKEHVTTVQTILDQ
HHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
LMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLLGL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796