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The map label for this gene is yeaJ

Identifier: 218695345

GI number: 218695345

Start: 2020474

End: 2021964

Strand: Direct

Name: yeaJ

Synonym: EC55989_1955

Alternate gene names: 218695345

Gene position: 2020474-2021964 (Clockwise)

Preceding gene: 218695344

Following gene: 218695346

Centisome position: 39.2

GC content: 45.81

Gene sequence:

>1491_bases
ATGAAATTGCACCATAGAATGCTCCGGCATTTTATCGCCGCAAGTGTCATTGTGCTGACATCTTCCTTCCTTATTTTTGA
ACTTGTCGCCAGCGACAGAGCAATGAGTGCCTATCTGCGCTATATCGTGCAGAAAGCAGACTCCTCCTTTCTTTATGATA
AGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGG
CGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAATACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACG
CGGCACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAG
TGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTC
AATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGG
AAGTATTGGTATCGATCGCAAAGATATTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGC
GTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTGTATGTTGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATT
AACAAAAACAATTTGCGGAATATCTTTTACACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGA
TACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTACGTCAGTTACGTCCATGACTTAC
CGGGCGGCATTCGTGTCTCGTTATCCATTGATATTCTTTACTTTATCACGTCTTCGTGGAAAAGCGTTCTGTTCTGGATT
TTGACGGCGTTAATTTTGCTGAATATGGTCCGGATGCACTTCCGTTTATACCAAAATGTGTCGCGAGAAAATATTAGTGA
TGCGATGACTGGACTGTATAATCGCAAAATTTTAACCCCTGAACTGGAGCAGCGGTTGCAGAAACTGGTGCAATCCGGTT
CTTCGGTGATGTTTATTGCAATTGACATGGACAAGTTAAAGCAAATAAATGACACCCTCGGTCATCAGGAGGGGGATTTA
GCGATTACGTTATTAGCTCAGGCGATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATT
CTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCG
CGCCGCAGAAAGAAATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCT
TCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGA

Upstream 100 bases:

>100_bases
CAGCGGCGCAGAGATGAGTATAATTAGCGCCCCTGTGCCAGGCCGCAATCGAACTTTATCTGGTTTTCTCGTTTCACTAA
CCGAAGGAGTGCCATTTATC

Downstream 100 bases:

>100_bases
TAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCG
GTTAATCGCTTTATTATCAC

Product: putative diguanylate cyclase

Products: NA

Alternate protein names: DGC

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQR
RAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDI
NKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWI
LTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDL
AITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKA
SDERLYVNKQNKNSRS

Sequences:

>Translated_496_residues
MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQR
RAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDI
NKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWI
LTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDL
AITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKA
SDERLYVNKQNKNSRS
>Mature_496_residues
MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQR
RAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDI
NKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWI
LTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDL
AITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKA
SDERLYVNKQNKNSRS

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain

Homologues:

Organism=Escherichia coli, GI87081974, Length=496, Percent_Identity=100, Blast_Score=1028, Evalue=0.0,
Organism=Escherichia coli, GI145693134, Length=169, Percent_Identity=33.1360946745562, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=220, Percent_Identity=30, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787262, Length=228, Percent_Identity=27.1929824561404, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1786584, Length=155, Percent_Identity=29.6774193548387, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=31.1377245508982, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=28.9940828402367, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YEAJ_ECOLI (P76237)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B64939
- RefSeq:   AP_002405.1
- RefSeq:   NP_416300.2
- ProteinModelPortal:   P76237
- DIP:   DIP-11789N
- MINT:   MINT-1239476
- STRING:   P76237
- EnsemblBacteria:   EBESCT00000002532
- EnsemblBacteria:   EBESCT00000016582
- GeneID:   946290
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5291
- KEGG:   eco:b1786
- EchoBASE:   EB3269
- EcoGene:   EG13496
- eggNOG:   COG2199
- GeneTree:   EBGT00050000008776
- HOGENOM:   HBG416250
- OMA:   GKEIIIN
- ProtClustDB:   CLSK880200
- BioCyc:   EcoCyc:G6972-MONOMER
- Genevestigator:   P76237
- InterPro:   IPR001054
- InterPro:   IPR000160
- SMART:   SM00267
- TIGRFAMs:   TIGR00254

Pfam domain/function: PF00990 GGDEF; SSF55073 A/G_cyclase

EC number: =2.7.7.65

Molecular weight: Translated: 56603; Mature: 56603

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50887 GGDEF

Important sites: ACT_SITE 425-425 BINDING 390-390 BINDING 399-399

Signals:

None

Transmembrane regions:

HASH(0x197804e8)-; HASH(0x1991853c)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDKYQNQSI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHCCCHH
AAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHCCCCCHHHH
VEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCEEEEEECC
WSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDI
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
NKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVS
CCCCCCEEEEECCCHHEEEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEE
LSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTP
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCH
ELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAI
HHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEE
RLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKA
EECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCHHHHHCC
SDERLYVNKQNKNSRS
CCCEEEEECCCCCCCH
>Mature Secondary Structure
MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDKYQNQSI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHCCCHH
AAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHCCCCCHHHH
VEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCEEEEEECC
WSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDI
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
NKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVS
CCCCCCEEEEECCCHHEEEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEE
LSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTP
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCH
ELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAI
HHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEE
RLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKA
EECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCHHHHHCC
SDERLYVNKQNKNSRS
CCCEEEEECCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503