Click here to switch to the map view.

The map label for this gene is pncA

Identifier: 218695327

GI number: 218695327

Start: 2000318

End: 2000959

Strand: Direct

Name: pncA

Synonym: EC55989_1937

Alternate gene names: 218695327

Gene position: 2000318-2000959 (Clockwise)

Preceding gene: 218695326

Following gene: 218695338

Centisome position: 38.8

GC content: 52.49

Gene sequence:

>642_bases
ATGCCCCATCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTACCGGAAGGTGA
CAGTACGGTGGAGGTTGCTAACCGCCTTATTGACTGGTGCCAGTCGCGCGGAGAAGCGGTTATCGCCAGTCAGGACTGGC
ACCCGGCGAATCACGGCAGTTTTGCCAGTCAGCACGGTGTAGAGCCTTATACGCCAGGCCAACTCGACGGTTTGCCACAA
ACCTTCTGGCCAGATCACTGTGTGCAGAACAGTGAAGGCGCACAATTACATCCGTTACTGAACCAAAAAGCGATCGCAGC
GGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTCGGCAGAAAACCTCTC
TCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGCTACTGACTATTGCGTGAAGTTTACC
GTGCTGGACGCGTTACAGTTAGGTTATAAGGTGAACGTGATTACCGATGGTTGTCGTGGCGTGAATATCCAGCCCCAGGA
CAGTGCACACGCGTTTATGGAGATGTCAGCAGCTGGGGCAACGCTATATACGCTGGCAGACTGGGAAGAGACACAGGGGT
AA

Upstream 100 bases:

>100_bases
CATTACCTGCTGAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCC
GGATGATTAAGGAGACTGCA

Downstream 100 bases:

>100_bases
TTTTACGCTGGCCTACAATTCTGTACTGGCATTGTAGGCCAAATAAAACACGTCAGTGGTACATCTGGCAATTGATGCCA
TCAACGAAAGATTTAATTCA

Product: nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRGEAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQ
TFWPDHCVQNSEGAQLHPLLNQKAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG

Sequences:

>Translated_213_residues
MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRGEAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQ
TFWPDHCVQNSEGAQLHPLLNQKAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG
>Mature_212_residues
PHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRGEAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQT
FWPDHCVQNSEGAQLHPLLNQKAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFTV
LDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=213, Percent_Identity=98.5915492957747, Blast_Score=437, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI193206735, Length=218, Percent_Identity=37.1559633027523, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17543130, Length=218, Percent_Identity=37.1559633027523, Blast_Score=123, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71999339, Length=211, Percent_Identity=30.3317535545024, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6321401, Length=196, Percent_Identity=33.1632653061224, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648247, Length=205, Percent_Identity=36.5853658536585, Blast_Score=118, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 23393; Mature: 23262

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRGEAVIASQDWHPANHGS
CCCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
FASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQKAIAAVFHKGENPLVDSY
CHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEECHHHHHHHHHHHHHCCCCCCHHHH
SAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFTVLDALQLGYKVNVITDGCRG
HHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCC
VNIQPQDSAHAFMEMSAAGATLYTLADWEETQG
CCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
PHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRGEAVIASQDWHPANHGS
CCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
FASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQKAIAAVFHKGENPLVDSY
CHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEECHHHHHHHHHHHHHCCCCCCHHHH
SAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFTVLDALQLGYKVNVITDGCRG
HHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCC
VNIQPQDSAHAFMEMSAAGATLYTLADWEETQG
CCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]