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The map label for this gene is pps

Identifier: 218695264

GI number: 218695264

Start: 1933366

End: 1935744

Strand: Reverse

Name: pps

Synonym: EC55989_1870

Alternate gene names: 218695264

Gene position: 1935744-1933366 (Counterclockwise)

Preceding gene: 218695268

Following gene: 218695258

Centisome position: 37.55

GC content: 54.48

Gene sequence:

>2379_bases
ATGTCCAACAATGGCTCGTCACCGCTGGTGCTTTGGTATAACCAACTCGGCATGAATGATGTAGACAGGGTTGGGGGCAA
AAATGCCTCCCTGGGTGAAATGATTACTAACCTTTCCGGAATGGGTGTTTCCGTTCCGAATGGTTTCGCCACAACCGCCG
ACGCGTTTAACCAGTTTCTGGACCAAAGCGGCGTAAACCAGCGCATTTATGAACTGCTGGATAAAACGGATATTGACGAT
GTTACTCAGCTTGCGAAAGCGGGCGCGCAAATCCGCCAGTGGATTATCGACACTCCCTTCCAGCCTGAGCTGGAAAACGC
CATCCGCGAAGCCTATGCACAGCTTTCCGCCGATGACGAAAACGCCTCGTTTGCGGTGCGTTCCTCCGCTACTGCAGAAG
ATATGCCGGACGCTTCTTTTGCCGGTCAGCAGGAAACCTTCCTCAACGTTCAGGGTTTTGACGCCGTTCTCGTGGCAGTG
AAACATGTATTTGCTTCTCTGTTTAACGATCGCGCCATCTCTTATCGTGTGCACCAGGGTTACGATCACCGTGGCGTGGC
GCTCTCCGCCGGTGTTCAGCGGATGGTGCGCTCCGACCTCGCATCTTCTGGCGTGATGTTCTCCATTGATACCGAATCCG
GCTTTGACCAGGTGGTGTTTATCACTTCCGCATGGGGCCTTGGGGAAATGGTCGTGCAGGGTGCGGTTAACCCGGATGAG
TTTTACGTCCATAAACCGACACTGGCGGCGAATCGCCCGGCTATCGTGCGCCGCACCATGGGATCGAAAAAAATCCGCAT
GGTTTATGCGCCGACCCAGGAGCACGGCAAGCAGGTTAAAATCGAAGACGTACCGCAGGAACAGCGTGACATCTTCTCGC
TGACCAACGAAGAAGTGCAGGAACTGGCAAAACAGGCCGTACAAATTGAGAAACACTACGGTCGCCCAATGGATATTGAG
TGGGCGAAAGATGGCCACACCGGCAAACTGTTCATTGTGCAGGCGCGTCCGGAAACCGTGCGCTCACGCGGTCAGGTCAT
GGAGCGTTATACGCTGCATTCACAGGGTAAGATTATCGCCGAAGGCCGTGCTATCGGTCATCGCATCGGTGCGGGTCCGG
TGAAAGTCATCCATGACATCAGCGAAATGAACCGCATCGAACCTGGTGACGTGCTGGTCACTGATATGACCGACCCGGAC
TGGGAACCGATCATGAAGAAAGCATCTGCCATCGTCACCAACCGTGGCGGTCGTACCTGTCACGCGGCGATCATCGCTCG
TGAACTGGGCATTCCGGCGGTAGTGGGCTGTGGAGATGCAACAGAACGGATGAAAGACGGTGAGAACGTCACTGTTTCTT
GTGCCGAAGGTGATACCGGTTACGTCTATGCGGAGTTGCTGGAATTTAGCGTGAAAAGCTCCAGCGTAGAAACGATGCCG
GATCTGCCGTTGAAAGTGATGATGAACGTCGGTAACCCGGACCGTGCTTTCGACTTCGCCTGTCTGCCGAACGAAGGCGT
GGGCCTTGCGCGTCTGGAATTTATCATCAACCGTATGATTGGCGTCCACCCACGCGCACTGCTTGAGTTTGACGATCAGG
AACCGCAGTTGCAAAACGAAATCCGCGAGATGATGAAAGGTTTTGATTCTCCGCGTGAATTTTACGTTGGTCGTCTGACT
GAAGGGATCGCGACGCTGGGTGCCGCGTTTTATCCGAAGCGCGTCATTGTCCGTCTCTCTGATTTTAAATCGAACGAATA
TGCCAACCTGGTCGGTGGTGAGCGTTACGAGCCAGATGAAGAGAACCCGATGCTCGGCTTCCGTGGCGCGGGCCGCTATG
TTTCCGACAGCTTCCGCGACTGTTTCGCGCTGGAGTGTGAAGCAGTGAAACGTGTGCGCAATGACATGGGGCTGACCAAC
GTTGAGATCATGATCCCGTTCGTGCGTACCGTAGATCAGGCGAAAGCGGTGGTTGAAGAACTGGCGCGTCAGGGGCTGAA
ACGTGGCGAGAACGGGCTGAAAATCATCATGATGTGTGAAATCCCGTCCAACGCCTTGCTGGCAGAGCAGTTCCTCGAAT
ATTTCGACGGCTTCTCAATTGGCTCAAACGACATGACACAGCTGGCGCTCGGTCTGGACCGTGACTCCGGAGTGGTGTCT
GAACTGTTCGATGAGCGCAACGATGCGGTGAAAGCATTGCTGTCGATGGCTATCCGTGCCGCGAAGAAACAGGGCAAATA
TGTCGGGATTTGCGGTCAGGGTCCGTCCGACCATGAAGACTTTGCCGCATGGTTGATGGAAGAGGGGATCGATAGCCTGT
CTCTGAACCCGGACACCGTGGTGCAAACCTGGTTAAGCCTGGCTGAACTGAAGAAATAA

Upstream 100 bases:

>100_bases
TTGAACGATTCACCGTTTTTTTCATCCGGTTAAATATGCAAAGATAAATGCGCAGAAATGTGTTTCTCAAACCGTTCATT
TATCACAAAAGGATTGTTCG

Downstream 100 bases:

>100_bases
AATAAATCCCCGGCGGCGTTCAGTCGCCGGGTTTATGTGATCCCCGAAGATGAAACTTATTCAATCTCTTCACAGACATC
CTGCGTTAATCGCCGCATAA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase

Number of amino acids: Translated: 792; Mature: 791

Protein sequence:

>792_residues
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDD
VTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
FYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIE
WAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPD
WEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMP
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLT
EGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTN
VEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK

Sequences:

>Translated_792_residues
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDD
VTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
FYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIE
WAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPD
WEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMP
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLT
EGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTN
VEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK
>Mature_791_residues
SNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDV
TQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVK
HVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDEF
YVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEW
AKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDW
EPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMPD
LPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTE
GIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNV
EIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSE
LFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1787994, Length=792, Percent_Identity=100, Blast_Score=1639, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=449, Percent_Identity=26.9487750556793, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1788726, Length=192, Percent_Identity=34.8958333333333, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI48994992, Length=190, Percent_Identity=31.5789473684211, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1789193, Length=405, Percent_Identity=25.9259259259259, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17564524, Length=331, Percent_Identity=25.9818731117825, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPSA_ECOLI (P23538)

Other databases:

- EMBL:   M69116
- EMBL:   X59381
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S20554
- RefSeq:   AP_002322.1
- RefSeq:   NP_416217.1
- ProteinModelPortal:   P23538
- SMR:   P23538
- DIP:   DIP-10552N
- IntAct:   P23538
- STRING:   P23538
- PhosSite:   P23538
- SWISS-2DPAGE:   P23538
- PRIDE:   P23538
- EnsemblBacteria:   EBESCT00000001671
- EnsemblBacteria:   EBESCT00000014612
- GeneID:   946209
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1692
- KEGG:   eco:b1702
- EchoBASE:   EB0752
- EcoGene:   EG10759
- eggNOG:   COG0574
- GeneTree:   EBGT00050000009028
- HOGENOM:   HBG284863
- OMA:   ERVGGKN
- ProtClustDB:   PRK06464
- BioCyc:   EcoCyc:PEPSYNTH-MONOMER
- BioCyc:   MetaCyc:PEPSYNTH-MONOMER
- BRENDA:   2.7.9.2
- Genevestigator:   P23538
- GO:   GO:0006094
- GO:   GO:0006094
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:3.20.20.60
- TIGRFAMs:   TIGR01418

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N; SSF52009 PEP_mobile; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.9.2

Molecular weight: Translated: 87436; Mature: 87305

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 421-421 ACT_SITE 751-751 BINDING 511-511 BINDING 578-578 BINDING 680-680 BINDING 701-701 BINDING 702-702 BINDING 703-703 BINDING 704-704

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFL
CCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHH
DQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDE
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
NASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQG
CCEEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
CCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCC
FYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQ
EEEECCCCCCCCCHHHHHHCCCCEEEEEECCCHHCCCEEEECCCCHHHHHHHHHCHHHHH
ELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIA
HHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEE
EGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASAIVTNRGGRTC
CCHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHH
HAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMP
HHHHHHHHCCCCEEEECCCHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCC
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNE
CCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEECCCCCCHHHHH
IREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHCCCCCCCCCCC
ENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEE
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
LARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHH
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTV
HHHHCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHH
VQTWLSLAELKK
HHHHHHHHHHCC
>Mature Secondary Structure 
SNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFL
CCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHH
DQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDE
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
NASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQG
CCEEEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
CCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCC
FYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQ
EEEECCCCCCCCCHHHHHHCCCCEEEEEECCCHHCCCEEEECCCCHHHHHHHHHCHHHHH
ELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIA
HHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEE
EGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASAIVTNRGGRTC
CCHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHH
HAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMP
HHHHHHHHCCCCEEEECCCHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCC
DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNE
CCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCHHEEECCCCCCHHHHH
IREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHCCCCCCCCCCC
ENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEE
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
LARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
HHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHH
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTV
HHHHCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHH
VQTWLSLAELKK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503