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The map label for this gene is ydhB

Identifier: 218695222

GI number: 218695222

Start: 1887495

End: 1888427

Strand: Reverse

Name: ydhB

Synonym: EC55989_1827

Alternate gene names: 218695222

Gene position: 1888427-1887495 (Counterclockwise)

Preceding gene: 218695225

Following gene: 218695220

Centisome position: 36.63

GC content: 53.91

Gene sequence:

>933_bases
ATGTGGTCAGAATATTCACTCGAAGTTGTTGATGCGGTAGCGCGTAATGGTAGTTTTAGCGCTGCGGCACAGGAGCTGCA
TCGCGTTCCTTCTGCGGTCAGCTATACCGTGCGTCAGCTGGAAGAGTGGCTGGCGGTGCCGCTCTTTGAACGACGGCACC
GTGATGTGGAGCTGACCGCTGCTGGAGCGTGGTTTCTCAAAGAAGGGCGCTCTGTTGTCAAAAAAATGCAGATCACCCGC
CAGCAATGTCAGCAGATAGCGAACGGCTGGCGCGGTCAGTTAGCTATCGCAGTGGATAATATTGTCAGGCCAGAACGTAC
ACGGCAGATGATCGTTGATTTTTATCGCCATTTTGATGATGTCGAACTTCTTGTCTTTCAGGAAGTGTTCAACGGTGTCT
GGGATGCGCTTTCCGACGGGCGCGTGGAACTGGCGATTGGCGCAACACGCGCGATTCCGGTAGGCGGTCGTTATGCCTTC
CGGGATATGGGGATGCTAAGCTGGAGTTGCGTTGTTGCCAGCCACCACCCGCTGGCGTTGATGGATGGCCCATTCAGCGA
TGATACGTTGCGCAACTGGCCGTCGTTGGTGCGCGAAGACACCTCGCGAACGCTACCTAAACGTATTACCTGGTTGCTGG
ATAACCAAAAAAGAGTTGTCGTGCCTGACTGGGAATCATCGGCAACCTGTATCTCGGCAGGATTATGCATAGGGATGGTG
CCAACACATTTCGCCAAACCGTGGCTTAATGAGGGGAAGTGGGTAGCACTTGAATTGGAAAACCCCTTCCCGGATTCGGC
ATGTTGTCTGACATGGCAGCAAAATGATATGTCGCCTGCGTTAACCTGGCTGCTTGAGTATCTCGGCGATAGTGAGACGC
TGAATAAGGAGTGGCTGCGGGAGCCGGAAGAGACTCCCGCAACGGGTGATTAA

Upstream 100 bases:

>100_bases
AATCTCTCAATTAAACACCGCCGCAAAGTGTAGGGAGTGAAATTAGTGTTGTGAAACGGTAATATTTGATGACTGGTTTC
AAAAATTTTGCAGGCAGACT

Downstream 100 bases:

>100_bases
CGACGATAGTCGCGGAACGGGCCGTCAGCCACGGAGCGGCGTTCAATCAAGCGCGGATGCACTTCAATGGACTGCGGTTC
TTCACGTTTGTTGACGATAC

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWLAVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITR
QQCQQIANGWRGQLAIAVDNIVRPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKRVVVPDWESSATCISAGLCIGMV
PTHFAKPWLNEGKWVALELENPFPDSACCLTWQQNDMSPALTWLLEYLGDSETLNKEWLREPEETPATGD

Sequences:

>Translated_310_residues
MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWLAVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITR
QQCQQIANGWRGQLAIAVDNIVRPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKRVVVPDWESSATCISAGLCIGMV
PTHFAKPWLNEGKWVALELENPFPDSACCLTWQQNDMSPALTWLLEYLGDSETLNKEWLREPEETPATGD
>Mature_310_residues
MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWLAVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITR
QQCQQIANGWRGQLAIAVDNIVRPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKRVVVPDWESSATCISAGLCIGMV
PTHFAKPWLNEGKWVALELENPFPDSACCLTWQQNDMSPALTWLLEYLGDSETLNKEWLREPEETPATGD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787949, Length=310, Percent_Identity=100, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1786508, Length=285, Percent_Identity=30.1754385964912, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1786713, Length=275, Percent_Identity=30.9090909090909, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI87082024, Length=249, Percent_Identity=31.3253012048193, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789492, Length=280, Percent_Identity=29.6428571428571, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI157672245, Length=173, Percent_Identity=33.5260115606936, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YDHB_ECO57 (P0ACR3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85773
- PIR:   H90924
- RefSeq:   NP_288095.1
- RefSeq:   NP_310395.1
- ProteinModelPortal:   P0ACR3
- SMR:   P0ACR3
- EnsemblBacteria:   EBESCT00000029011
- EnsemblBacteria:   EBESCT00000059032
- GeneID:   912285
- GeneID:   961628
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2682
- KEGG:   ecs:ECs2368
- GeneTree:   EBGT00050000008332
- HOGENOM:   HBG685425
- OMA:   KLHKVPS
- ProtClustDB:   PRK11074
- BioCyc:   ECOL83334:ECS2368-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 35250; Mature: 35250

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWLAVPLFERRHRDVELTA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEE
AGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIVRPERTRQMIVDFYRHFDD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHHCCCC
VELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAFRDMGMLSWSCVVASHHPLAL
HHHHHHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCHHHHHCCCCEEEEEEECCCCEEE
MDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKRVVVPDWESSATCISAGLCIGMV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCHHHHHHHHHHCC
PTHFAKPWLNEGKWVALELENPFPDSACCLTWQQNDMSPALTWLLEYLGDSETLNKEWLR
HHHHHCHHCCCCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHC
EPEETPATGD
CCCCCCCCCC
>Mature Secondary Structure
MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWLAVPLFERRHRDVELTA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEE
AGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIVRPERTRQMIVDFYRHFDD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHHCCCC
VELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAFRDMGMLSWSCVVASHHPLAL
HHHHHHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCHHHHHCCCCEEEEEEECCCCEEE
MDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKRVVVPDWESSATCISAGLCIGMV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCHHHHHHHHHHCC
PTHFAKPWLNEGKWVALELENPFPDSACCLTWQQNDMSPALTWLLEYLGDSETLNKEWLR
HHHHHCHHCCCCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHC
EPEETPATGD
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796