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The map label for this gene is yneJ

Identifier: 218695064

GI number: 218695064

Start: 1730936

End: 1731817

Strand: Direct

Name: yneJ

Synonym: EC55989_1659

Alternate gene names: 218695064

Gene position: 1730936-1731817 (Clockwise)

Preceding gene: 218695057

Following gene: 218695065

Centisome position: 33.58

GC content: 53.74

Gene sequence:

>882_bases
ATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATTACCCAGGCTGCAGCAAAAGTGCATCG
CGTACCGTCGAATCTCACTACCCGTCTACGCCAGCTGGAAACAGAACTGGGGGTAGATCTGTTTATTCGCGAGAATCAGC
GTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGC
GTTGTCGCTGGCGATGAGCCGCAAGGTTTATTTTCTCTTGGTTCGCTGGAAAGCACCGCAGCAGTGCGCATTCCGGCCAC
GCTGGCGGAATTCAACCATCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGAAGGAG
TGCTGGAGGGAAAACTGAATGCGGCATTTATTGATGGGCCCATTAACCATACTGCCATTGACGGGATACCGGTATACCGC
GAGGAGCTGATGATCGTCACGCCACAAGGTCATGCGCCAGTAATCCGCGCCAGTCAGGTTAACGGCAGTAACATTTATGC
CTTCCGCGCCAATTGTTCGTATCGTCGCCACTTCGAGAGCTGGTTTCATGCTGACGGTGCCGCTCCGGGAACTATTCACG
AGATGGAGTCGTATCACGGGATGTTGGCCTGTGTGGTCGCAGGAGCAGGCATTGCGCTTATTCCACGCTCTATGCTGGAA
AGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCG
TCGTGGTGCGAAAACGCGTCCGCTTGAGGCATTTATTCAACTACTGGATGTGCCTGACTCGGCAAAACAGGGTTACCAAT
GA

Upstream 100 bases:

>100_bases
CGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTT
ATTCACGAATCGAGAATGTT

Downstream 100 bases:

>100_bases
GCTATTTTTGATAGTTCTTGCAGCTCAAATGCCCTATATAGTGATGTTCCGATGACTTATGACTATATGGGGCAAATATG
GTTACGCCAGTAAGCATCAG

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ

Sequences:

>Translated_293_residues
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ
>Mature_293_residues
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=293, Percent_Identity=97.9522184300341, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=26.3698630136986, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082132, Length=287, Percent_Identity=23.3449477351916, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=25.390625, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790262, Length=290, Percent_Identity=23.7931034482759, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=283, Percent_Identity=23.6749116607774, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788481, Length=179, Percent_Identity=31.2849162011173, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=21.5277777777778, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787879, Length=285, Percent_Identity=23.859649122807, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790399, Length=289, Percent_Identity=21.7993079584775, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788297, Length=258, Percent_Identity=24.4186046511628, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787601, Length=286, Percent_Identity=21.6783216783217, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32422; Mature: 32422

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAVRIPATLAEFNHRYPKIQF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCHHHHHHCCCCCEEEE
SLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYREELMIVTPQGHAPVIRASQV
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCEECCCCEECCCEEEECCCCCCCEEEEEEC
NGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ
HCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAVRIPATLAEFNHRYPKIQF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCHHHHHHCCCCCEEEE
SLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYREELMIVTPQGHAPVIRASQV
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCEECCCCEECCCEEEECCCCCCCEEEEEEC
NGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ
HCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]