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The map label for this gene is ydeN

Identifier: 218695035

GI number: 218695035

Start: 1693212

End: 1694894

Strand: Reverse

Name: ydeN

Synonym: EC55989_1630

Alternate gene names: 218695035

Gene position: 1694894-1693212 (Counterclockwise)

Preceding gene: 218695036

Following gene: 218695034

Centisome position: 32.88

GC content: 43.97

Gene sequence:

>1683_bases
ATGAAGTCTGCATTAAAGAAAAGTGTCGTAAGTACCTCGATATCTTTGATACTGGCATCTGGTATGGCTGCATTTGCTGC
TCATGCGGCAGATGATGTAAAGCTGAAAGCAACCAAAACAAACGTTGCTTTCTCAGACTTTACGCCGACTGAATACAGTA
CCAAAGGAAAGCCAAATATTATCGTACTAACCATGGATGATCTTGGTTATGGACAACTTCCTTTTGATAAGGGATCTTTT
GACCCAAAAACAATGGAAAATCGTGAAGTTGTCGATACCTACAAAATAGGGATAGATAAAGCCATTGAAGCTGCACAAAA
ATCAACGCCGACGCTCCTTTCATTAATGGATGAAGGCGTACGGTTTACTAACGGCTATGTGGCACACGGTGTTTCCGGCC
CCTCCCGTGCCGCAATAATGACCGGTCGAGCTCCCGCCCGCTTTGGTGTCTATTCCAATACCGATGCTCAGGATGGTATT
CCGCTAACAGAAACTTTCTTGCCTGAATTATTCCAGAATCATGGTTATTACACTGCAGCAGTAGGTAAATGGCACTTGTC
AAAAATCAGTAATGTGCCGGTACCGGAAGATAAACAAACGCGTGACTATCATGACAACTTCACCACATTTTCTGCGGAAG
AATGGCAACCTCAAAACCGTGGCTTTGATTACTTTATGGGATTCCACGCTGCAGGAACGGCATATTACAACTCCCCTTCA
CTGTTCAAAAATCGTGAACGTGTCCCCGCAAAAGGTTATATCAGCGATCAGTTAACCGATGAGGCAATTGGCGTTGTTGA
TCGTGCCAAAACACTTGACCAGCCTTTTATGCTTTACCTGGCTTATAATGCTCCGCACCTGCCAAATGATAATCCTGCAC
CGGATCAATATCAGAAGCAATTTAATACCGGTAGTCAAACAGCAGATAACTACTACGCTTCCGTTTATTCTGTTGATCAG
GGTGTAAAACGCATTCTCGAACAACTGAAGAAAAACGGACAGTATGACAATACAATTATTCTCTTTACCTCCGATAATGG
TGCGGTTATCGATGGTCCTCTGCCGCTGAACGGGGCGCAAAAAGGCTATAAGAGTCAGACCTATCCTGGCGGTACTCACA
CCCCAATGTTTATGTGGTGGAAAGGAAAACTTCAACCCGGTAATTATGACAAGCTGATTTCCGCAATGGATTTCTACCCG
ACAGCTCTTGATGCAGCCGATATCAGCATTCCAAAAGACCTTAAGCTGGATGGCGTTTCCTTGCTGCCCTGGTTGCAAGA
TAAGAAACAAGGCGAGCCACATAAAAATCTGACCTGGATAACCTCTTATTCTCACTGGTTTGACGAGGAAAATATTCCAT
TCTGGGATAATTACCACAAATTTGTTCGCCATCAGTCAGACGATTACCCGCATAACCCCAACACTGAGGACTTAAGCCAA
TTCTCTTATACGGTGAGAAATAACGATTATTCGCTTGTCTATACAGTAGAAAACAATCAGTTAGGTCTCTACAAACTGAC
GGATCTACAGCAAAAAGATAACCTTGCCGCCGCCAATCCGCAGGTCGTTAAAGAGATGCAAGGCGTGGTAAGAGAGTTTA
TCGACAGCAGCCAGCCACCGCTTAGCGAAGTAAATCAGGAGAAGTTTAACAATATCAAGAAAGCACTAAGCGAAGCGAAA
TAA

Upstream 100 bases:

>100_bases
TAAATCTGCTATCTCTTTATGTGACTAACTTCACTTACATCCACTTATTTCTCTTCGTAAAATTACTTCGGAATTAAGTA
CAATAAGAAGAGGAACATTT

Downstream 100 bases:

>100_bases
CTAAACCTTCATGCGGCGGAATTTTCCGCCGCCTTATTGAGCGAGATAGCGATGCACGTTACAGCCAAGCCCTCCAGTTT
TCAATGTAATCTCAAATGTG

Product: putative sulfatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAFSDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSF
DPKTMENREVVDTYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPS
LFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQ
GVKRILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYP
TALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQ
FSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK

Sequences:

>Translated_560_residues
MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAFSDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSF
DPKTMENREVVDTYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPS
LFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQ
GVKRILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYP
TALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQ
FSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK
>Mature_560_residues
MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAFSDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSF
DPKTMENREVVDTYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPS
LFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQ
GVKRILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYP
TALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQ
FSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK

Specific function: Unknown

COG id: COG3119

COG function: function code P; Arylsulfatase A and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sulfatase family

Homologues:

Organism=Homo sapiens, GI4503899, Length=503, Percent_Identity=27.2365805168986, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI45430057, Length=453, Percent_Identity=26.7108167770419, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI146229331, Length=412, Percent_Identity=26.9417475728155, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI146229329, Length=412, Percent_Identity=26.9417475728155, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI6005990, Length=412, Percent_Identity=26.9417475728155, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI146229324, Length=412, Percent_Identity=26.9417475728155, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI59797060, Length=357, Percent_Identity=26.890756302521, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI109389362, Length=346, Percent_Identity=27.4566473988439, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI38569407, Length=345, Percent_Identity=26.6666666666667, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI38569405, Length=386, Percent_Identity=25.3886010362694, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI31742482, Length=180, Percent_Identity=31.6666666666667, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI58743319, Length=457, Percent_Identity=23.8512035010941, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI146229327, Length=311, Percent_Identity=25.08038585209, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI157266309, Length=183, Percent_Identity=29.5081967213115, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI53831991, Length=185, Percent_Identity=30.2702702702703, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI71852586, Length=180, Percent_Identity=30, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI71852584, Length=180, Percent_Identity=30, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI87081924, Length=560, Percent_Identity=100, Blast_Score=1161, Evalue=0.0,
Organism=Escherichia coli, GI1790233, Length=450, Percent_Identity=26.8888888888889, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI115533416, Length=515, Percent_Identity=24.4660194174757, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI115533418, Length=465, Percent_Identity=25.5913978494624, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17568795, Length=353, Percent_Identity=24.929178470255, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17559078, Length=172, Percent_Identity=32.5581395348837, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24666109, Length=416, Percent_Identity=28.8461538461538, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24666175, Length=408, Percent_Identity=28.1862745098039, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281366397, Length=397, Percent_Identity=28.9672544080605, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281366395, Length=397, Percent_Identity=28.9672544080605, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24666163, Length=397, Percent_Identity=28.9672544080605, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281363223, Length=378, Percent_Identity=26.1904761904762, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21356831, Length=401, Percent_Identity=22.1945137157107, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YDEN_ECOLI (P77318)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E64903
- RefSeq:   AP_002121.1
- RefSeq:   NP_416015.2
- ProteinModelPortal:   P77318
- SMR:   P77318
- DIP:   DIP-11682N
- STRING:   P77318
- PhosSite:   P77318
- EnsemblBacteria:   EBESCT00000001979
- EnsemblBacteria:   EBESCT00000015602
- GeneID:   945957
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5243
- KEGG:   eco:b1498
- EchoBASE:   EB3557
- EcoGene:   EG13796
- eggNOG:   COG3119
- GeneTree:   EBGT00050000009139
- HOGENOM:   HBG467794
- OMA:   QKDNIAA
- ProtClustDB:   CLSK880035
- BioCyc:   EcoCyc:G6788-MONOMER
- Genevestigator:   P77318
- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR000917
- Gene3D:   G3DSA:3.40.720.10

Pfam domain/function: PF00884 Sulfatase; SSF53649 Alkaline_phosphatase_core

EC number: 3.1.6.- [C]

Molecular weight: Translated: 62803; Mature: 62803

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00523 SULFATASE_1; PS00149 SULFATASE_2

Important sites: ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAFSDFTPTEYSTKGKPNI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECEEEECCCCCCCCCCCCCCCE
IVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKSTPTLLSLMDEGV
EEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHCCCCHHHHHHHCCC
RFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETFLPELFQNHGYYTAA
EECCCEEEECCCCCCCEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEE
VGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPS
ECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEEECCCCEEECCCH
LFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQ
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHH
FNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQ
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCCCC
KGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVS
CCCCCCCCCCCCCCCEEEEEECEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
LLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQ
ECCCHHHHCCCCCCCCEEEEECHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
FSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPP
HEEEEECCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
LSEVNQEKFNNIKKALSEAK
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAFSDFTPTEYSTKGKPNI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECEEEECCCCCCCCCCCCCCCE
IVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKSTPTLLSLMDEGV
EEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHCCCCHHHHHHHCCC
RFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETFLPELFQNHGYYTAA
EECCCEEEECCCCCCCEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEE
VGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPS
ECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEEECCCCEEECCCH
LFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQ
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHH
FNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQ
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCCCC
KGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVS
CCCCCCCCCCCCCCCEEEEEECEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
LLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQ
ECCCHHHHCCCCCCCCEEEEECHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
FSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPP
HEEEEECCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
LSEVNQEKFNNIKKALSEAK
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503