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The map label for this gene is vatD [H]

Identifier: 218694986

GI number: 218694986

Start: 1625720

End: 1626529

Strand: Direct

Name: vatD [H]

Synonym: EC55989_1577

Alternate gene names: 218694986

Gene position: 1625720-1626529 (Clockwise)

Preceding gene: 218694985

Following gene: 218694987

Centisome position: 31.54

GC content: 33.58

Gene sequence:

>810_bases
ATGAAAGTTCAATTGTACAAATTCACGGAAGATAAAAATAAAACCCTTACCTTCAGATGGACGAAAAAACACTTTGAGTT
TTGTATGGATAATAAAATCTTTTTAAATCATAAGGGGAAGAAGTCGTATAAAGAAAGGAATTTATTTCTTTTCTCAAAAG
GTGACAAGATAACAATTGAAGATAATGTTATTGCTGAAGAATATTCAACAATGCCTGTGAAAAATTTCTCCAGTGTCGGC
GCTTTTTCATTTCCGACCTGCCATTTTTCAGGGAATATACGTATAGGTCGATTTTGTAGTATTGCATCAAATGTAAAAAT
AATGGGTGGAAATCATCCTTTGAATCGTTTTACTACTCACATGATGACTTATAACGGTGAATTTGATAAGTTTGCTATGA
GTGAATTTGAACGTAGCTGGACATTGAAACCATTCATTACCAAACCTGAAAATCCGATTATAGGAAATGATGTCTGGATA
GGAAATGATGTTGTATTGAAAGGTGGCATCGCAATAGGTGATGGTGCTGTTATTGCTGCCAATTCCGTTGTTACCAAAGA
TGTGCCCCCATATGCCATAGTTGCAGGAGTTCCTGCAAAAATAATCAGATTTAGGTTTGATTCAAATGTTATTGATGAAC
TGTTGAGAATAAAATGGTGGAATTATAACTACTCAGATCTTCCGGATAATAACAAGTGTGATGATATAAATTATTTTGTT
GAAGAGATGAATCGATTGATATCAGATGGAAATATTCAAGAGAGGGACTATAAAAAATTCAATCTTTCTGAGGTATTCAG
AGGCCTATAA

Upstream 100 bases:

>100_bases
TAAATGTTTGATGGCGATTTTTCATCCTGTCACAGTTGATTAGCATAAGGCTTTAGAGTATCGTTATTAAACTAATAATT
TATAAGTAGAAGTATTAACA

Downstream 100 bases:

>100_bases
TTATCTGTTTTGCATGATAAATGGGCAAGATAACCAGGGCGGGGTTTGCAATTTCAATGGGGCAGCGTCATCAAGGAGGT
TGCAAACATCCTGCCATGCA

Product: putative acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: Virginiamycin acetyltransferase D; Vat(D) [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYKERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVG
AFSFPTCHFSGNIRIGRFCSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYSDLPDNNKCDDINYFV
EEMNRLISDGNIQERDYKKFNLSEVFRGL

Sequences:

>Translated_269_residues
MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYKERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVG
AFSFPTCHFSGNIRIGRFCSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYSDLPDNNKCDDINYFV
EEMNRLISDGNIQERDYKKFNLSEVFRGL
>Mature_269_residues
MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYKERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVG
AFSFPTCHFSGNIRIGRFCSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYSDLPDNNKCDDINYFV
EEMNRLISDGNIQERDYKKFNLSEVFRGL

Specific function: Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=111, Percent_Identity=37.8378378378378, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786664, Length=111, Percent_Identity=35.1351351351351, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788345, Length=137, Percent_Identity=32.8467153284672, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 31073; Mature: 31073

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYKERNLFLFSKGDKITIE
CEEEEEEECCCCCCEEEEEEEHHHHHHHCCCEEEEECCCCCCHHHCCEEEEECCCEEEEE
DNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMGGNHPLNRFTTH
CCEEEHHHCCCCCCCCCCCCCCCCCEEEEECCEEEEEEEECCCCEEEEECCCCHHHHEEE
MMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKGGIAIGDGAVIAA
EEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCEEEECCEEECCCCEEEE
NSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYSDLPDNNKCDDINYFV
CCEEECCCCCEEEEECCCHHEEEEECCCHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHH
EEMNRLISDGNIQERDYKKFNLSEVFRGL
HHHHHHHCCCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYKERNLFLFSKGDKITIE
CEEEEEEECCCCCCEEEEEEEHHHHHHHCCCEEEEECCCCCCHHHCCEEEEECCCEEEEE
DNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMGGNHPLNRFTTH
CCEEEHHHCCCCCCCCCCCCCCCCCEEEEECCEEEEEEEECCCCEEEEECCCCHHHHEEE
MMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKGGIAIGDGAVIAA
EEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCEEEECCEEECCCCEEEE
NSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYSDLPDNNKCDDINYFV
CCEEECCCCCEEEEECCCHHEEEEECCCHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHH
EEMNRLISDGNIQERDYKKFNLSEVFRGL
HHHHHHHCCCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8257133 [H]