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The map label for this gene is puuD

Identifier: 218694874

GI number: 218694874

Start: 1502846

End: 1503610

Strand: Direct

Name: puuD

Synonym: EC55989_1460

Alternate gene names: 218694874

Gene position: 1502846-1503610 (Clockwise)

Preceding gene: 218694857

Following gene: 218694875

Centisome position: 29.15

GC content: 52.29

Gene sequence:

>765_bases
ATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCT
GCAAGAAAAGTACCTGAATGCCATCATCCACGCAGGCGGCTTGCCAATTGCGCTACCACATGCGCTGGCGGAACCGTCGT
TACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGT
GAAAACGGCGATGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGCACTCGAAAGGCG
CATTCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTAGTGGTGGCAACCGGTGGTTCGTTGCATCGCAAGCTGTGCG
AACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAG
GTTGTAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGC
GAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATC
CTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTT
ATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGA

Upstream 100 bases:

>100_bases
CAAAAATGAAAACTCACTGCTAGATTGAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAG
TCCACTTTGTGAGATTGAGC

Downstream 100 bases:

>100_bases
CCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAG
CAGGGGCTTTCACAACGTCG

Product: gamma-glutamyl-gamma-aminobutyrate hydrolase

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYG
ENGDEPDADPGRDLLSMAIINAALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
VVEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRILFEGF
ITACQHHIAEKQRL

Sequences:

>Translated_254_residues
MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYG
ENGDEPDADPGRDLLSMAIINAALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
VVEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRILFEGF
ITACQHHIAEKQRL
>Mature_254_residues
MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYG
ENGDEPDADPGRDLLSMAIINAALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
VVEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRILFEGF
ITACQHHIAEKQRL

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=254, Percent_Identity=99.6062992125984, Blast_Score=513, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 27983; Mature: 27983

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLP
CCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEECHHHHCCHHHHHHHHH
KLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINAALERRIPIFAICRGLQEL
HHCCEECCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVVEGGLLSALLPECSNFWVN
HHHCCCHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
SLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRILFEGF
HHCCCCCEEECCCEEEECCCCCHHHHHHHHHCCCEEECEEECCCCCCHHHHHHHHHHHHH
ITACQHHIAEKQRL
HHHHHHHHHHHCCC
>Mature Secondary Structure
MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLP
CCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEECHHHHCCHHHHHHHHH
KLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINAALERRIPIFAICRGLQEL
HHCCEECCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVVEGGLLSALLPECSNFWVN
HHHCCCHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
SLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRILFEGF
HHCCCCCEEECCCEEEECCCCCHHHHHHHHHCCCEEECEEECCCCCCHHHHHHHHHHHHH
ITACQHHIAEKQRL
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]