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The map label for this gene is ndh

Identifier: 218694642

GI number: 218694642

Start: 1276231

End: 1277535

Strand: Direct

Name: ndh

Synonym: EC55989_1221

Alternate gene names: 218694642

Gene position: 1276231-1277535 (Clockwise)

Preceding gene: 218694641

Following gene: 218694643

Centisome position: 24.76

GC content: 52.72

Gene sequence:

>1305_bases
TTGACTACGCCATTGAAAAAGATTGTGATTGTCGGCGGCGGTGCTGGTGGGCTGGAAATGGCAACACAGCTGGGGCATAA
GCTGGGACGTAAGAAAAAAGCCAAAATTACGCTGGTCGATCGTAACCACAGCCACCTGTGGAAACCGCTGCTGCACGAAG
TGGCGACTGGCTCGCTTGATGAAGGCGTCGATGCGTTGAGCTATCTGGCCCATGCGCGCAATCATGGTTTCCAGTTCCAG
CTGGGTTCCGTCATTGATATTGATCGTGAAGCGAAAACAATCACTATTGCAGAACTGCGCGATGAGAAAGGTGAACTGCT
GGTTCCGGAACGTAAAATCGCCTATGACACCCTGGTAATGGCGCTGGGTAGCACCTCTAACGATTTCAATACGCCAGGTG
TCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTTCACCAGGAGATGCTGAATCTCTTCCTGAAA
TACTCCGCCAACCTGGGCGCAAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGC
TGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGG
TAGAAGCGGGAGAACGTATTTTGCCTGCATTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGC
GTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAATATATTGAGGC
TGATCTGATGGTGTGGGCAGCCGGGATCAAAGCGCCAGACTTCCTGAAAGATATCGGTGGTCTTGAAACTAACCGTATCA
ACCAGCTGGTGGTGGAACCGACGCTGCAAACCACCCGCGATCCAGACATTTACGCTATTGGCGACTGCGCGTCATGCCCG
CGTCCGGAAGGGGGCTTTGTTCCGCCGCGTGCTCAGGCTGCACACCAGATGGCGACTTGCGCAATGAACAACATACTGGC
GCAGATGAACGGTAAGCCGCTGAAAAATTATCAGTATAAAGATCATGGTTCGCTGGTATCGCTGTCGAACTTCTCCACCG
TCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTA
TACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTAT
CCGTCCGCGTTTGAAGTTGCATTAA

Upstream 100 bases:

>100_bases
AATAGCGAAGAACATTTTCATTGCTGTAACCTGTTGTTAATTAAGAGCTATGTTAATAACCATTAATTAACAATTGGTTA
ATAAATTTAAGGGGGTCACG

Downstream 100 bases:

>100_bases
TCGACGTACACTGGCGGATGTGGCATAAACGCCTTATCCGCCCTTGAGGAACAGCGCGATCGGCAGCCGCGTCGCATCAG
GCATTCCTTCAGACTCCTCC

Product: respiratory NADH dehydrogenase 2/cupric reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQ
LGSVIDIDREAKTITIAELRDEKGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELTKLG
VRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCP
RPEGGFVPPRAQAAHQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISL
YRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH

Sequences:

>Translated_434_residues
MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQ
LGSVIDIDREAKTITIAELRDEKGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELTKLG
VRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCP
RPEGGFVPPRAQAAHQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISL
YRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH
>Mature_433_residues
TTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQL
GSVIDIDREAKTITIAELRDEKGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKY
SANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELTKLGV
RVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPR
PEGGFVPPRAQAAHQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISLY
RMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH

Specific function: Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation

COG id: COG1252

COG function: function code C; NADH dehydrogenase, FAD-containing subunit

Gene ontology:

Cell location: Cell membrane

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787352, Length=434, Percent_Identity=100, Blast_Score=896, Evalue=0.0,
Organism=Escherichia coli, GI1789065, Length=358, Percent_Identity=26.536312849162, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320118, Length=349, Percent_Identity=26.3610315186246, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323515, Length=464, Percent_Identity=26.0775862068966, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323794, Length=349, Percent_Identity=28.3667621776504, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHNA_ECOLI (P00393)

Other databases:

- EMBL:   V00306
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A00461
- RefSeq:   AP_001735.1
- RefSeq:   NP_415627.1
- PDB:   1OZK
- PDBsum:   1OZK
- ProteinModelPortal:   P00393
- SMR:   P00393
- DIP:   DIP-10325N
- STRING:   P00393
- PeroxiBase:   5949
- EnsemblBacteria:   EBESCT00000002548
- EnsemblBacteria:   EBESCT00000014552
- GeneID:   946792
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1095
- KEGG:   eco:b1109
- EchoBASE:   EB0643
- EcoGene:   EG10649
- eggNOG:   COG1252
- GeneTree:   EBGT00050000009316
- HOGENOM:   HBG668030
- OMA:   HRFRTEM
- ProtClustDB:   CLSK874586
- BioCyc:   EcoCyc:NADH-DHII-MONOMER
- BioCyc:   MetaCyc:NADH-DHII-MONOMER
- Genevestigator:   P00393
- GO:   GO:0006810
- InterPro:   IPR013027
- InterPro:   IPR001327
- PRINTS:   PR00368

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2

EC number: =1.6.99.3

Molecular weight: Translated: 47359; Mature: 47228

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEVATGSLD
CCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHH
EGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKGELLVPERKIAYDTLVM
HHHHHHHHHHHHHCCCCEEECCCEEECCCCCCEEEEEEECCCCCCEECCCHHHHHHHHHH
ALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYSANLGANGKVNIAIVGGGA
HHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCC
TGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELTKLG
CCEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHCCCCCCCCCHHHHHHHHHHH
VRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIGGLETNRINQLVVEP
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCCCCCCEEEECC
TLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQMATCAMNNILAQMNGKPLKNYQYK
CHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISLYRMHQIALHGYFKTGLMMLV
CCCCEEEECCHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSINRVIRPRLKLH
HHHHHHHCCHHCCC
>Mature Secondary Structure 
TTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEVATGSLD
CCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHH
EGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKGELLVPERKIAYDTLVM
HHHHHHHHHHHHHCCCCEEECCCEEECCCCCCEEEEEEECCCCCCEECCCHHHHHHHHHH
ALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYSANLGANGKVNIAIVGGGA
HHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCC
TGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELTKLG
CCEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHCCCCCCCCCHHHHHHHHHHH
VRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIGGLETNRINQLVVEP
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCCCCCCEEEECC
TLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQMATCAMNNILAQMNGKPLKNYQYK
CHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISLYRMHQIALHGYFKTGLMMLV
CCCCEEEECCHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSINRVIRPRLKLH
HHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6265208; 8905232; 9278503