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The map label for this gene is insE

Identifier: 218694565

GI number: 218694565

Start: 1205454

End: 1205762

Strand: Reverse

Name: insE

Synonym: EC55989_1142

Alternate gene names: 218694565

Gene position: 1205762-1205454 (Counterclockwise)

Preceding gene: 218694572

Following gene: 218694564

Centisome position: 23.39

GC content: 50.81

Gene sequence:

>309_bases
GTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCT
GAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAGCGCTACAACTGGCGCA
GTAAACAGCAAAATCAGCAGACGTCTTCTGAACGTGAACTGGAGATGTCTACCGAGATTGCACGTCTCAAACGCCAGCTG
GCAGAACGGGATGAAGAGCTGGCTATCCTCCAAAAGGCCGCGACATACTTCGCGAAGCGCCTGAAATGA

Upstream 100 bases:

>100_bases
TAATCATCGTCTGCAGTTGTTCGCGGTTGATGCCTTTAACCGCCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGT
GAGTAAACTCTCAGTCAGAG

Downstream 100 bases:

>100_bases
AGTATGTCTTTATTGAAAAACATCAGGCTGAGTTCAGCATCAAAGCAATGTGCCGCGTGCTCCGGGTGGCCCGCAGCGGC
TGGTATACGTGGTGTCAGCG

Product: IS3 element protein InsE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 102; Mature: 101

Protein sequence:

>102_residues
MTHMTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQRYNWRSKQQNQQTSSERELEMSTEIARLKRQL
AERDEELAILQKAATYFAKRLK

Sequences:

>Translated_102_residues
MTHMTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQRYNWRSKQQNQQTSSERELEMSTEIARLKRQL
AERDEELAILQKAATYFAKRLK
>Mature_101_residues
THMTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQRYNWRSKQQNQQTSSERELEMSTEIARLKRQLA
ERDEELAILQKAATYFAKRLK

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: COG2963

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 8 family [H]

Homologues:

Organism=Escherichia coli, GI226510958, Length=99, Percent_Identity=98.989898989899, Blast_Score=193, Evalue=2e-51,
Organism=Escherichia coli, GI226510933, Length=99, Percent_Identity=98.989898989899, Blast_Score=193, Evalue=2e-51,
Organism=Escherichia coli, GI226510930, Length=99, Percent_Identity=98.989898989899, Blast_Score=193, Evalue=2e-51,
Organism=Escherichia coli, GI226510928, Length=99, Percent_Identity=98.989898989899, Blast_Score=193, Evalue=2e-51,
Organism=Escherichia coli, GI226510927, Length=99, Percent_Identity=98.989898989899, Blast_Score=193, Evalue=2e-51,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002514 [H]

Pfam domain/function: PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 11956; Mature: 11825

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHMTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQRYNWRSKQQNQQ
CCCCCCHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TSSERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THMTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQRYNWRSKQQNQQ
CCCCCHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TSSERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]