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The map label for this gene is pgaA

Identifier: 218694560

GI number: 218694560

Start: 1198297

End: 1200720

Strand: Reverse

Name: pgaA

Synonym: EC55989_1135

Alternate gene names: 218694560

Gene position: 1200720-1198297 (Counterclockwise)

Preceding gene: 218694564

Following gene: 218694559

Centisome position: 23.29

GC content: 47.28

Gene sequence:

>2424_bases
ATGTATTCAAGTAGCAGAAAAAGGTGCCCGAAAACCAAATGGGCTTTGAAACTTCTTACTGCCGCATTTTTAGCAGCGAG
TCCCGCGGCGAAGAGTGCTGTTAATAACGCCTATGATGCATTGATTATTGAAGCTCGCAAGGGTAATACTCAGCCAGCTT
TGTCATGGTTTGCACTAAAATCAGCACTCAGCAATAACCAAATTGCTGACTGGTTACAGATTGCCTTATGGGCCGGGCAA
GATAAACAGGTTATTACCGTTTACAACCGCTACCGTCATCAGCAATTACCAGCGCGTGGTTATGCAGCTGTCGCCGTCGC
TTATCGTAACCTGCAACAATGGCAAAACTCGCTTACACTGTGGCAAAAGGCGCTCTCTCTGGAGCCGCAAAATAAGGATT
ATCAACGGGGACAAATTTTAACCCTGGCAGATGCTGGTCACTATGATACTGCGCTGGTTAAACTTAAGCAGCTTAACTCT
GGAGCACCGGACAAAGCCAATTTACTCGCAGAAGCCTATATCTATAAACTGGCGGGGCGTCATCAGGATGAATTACGGGC
GATGACAGAGTCATTACCTGAAAATGCATCTACGCAACAATATCCCACAGAATACGTGCAGGCATTACGTAATAATCAAC
TTGCTGCCGCGATTGACGATGCCAATTTAACGCCAGATATTCGCGCTGATATTCATGCCGAACTGGTCAGACTGTCGTTT
ATGCCTACGCGCAGTGAAAGTGAACGTTATGCCATTGCCGATCGCGCCCTCGCCCAATACGCTGCATTAGAAATTCTGTG
GCACGATAACCCAGACCGTACTGCCCAGTACCAGCGTATTCAGGTTGATCATCTTGGCGCGTTATTAACTCGCGATCGTT
ATAAAGACGTTATTTCTCACTATCAGCGATTAAAAAAGACGGGGCAAATTATTCCGCCCTGGGGGCAATATTGGGTTGCA
TCGGCTTATCTCAAAGATCATCAGCCGAAAAAAGCACAGTCAATAATGACCGAGCTCTTTTATCACAAGGAGACCATTGC
CCCGGATTTATCCGATGAAGAACTTGCGGATCTCTTTTACAGCCACCTGGAGAGTGAAAATTATCCGGGCGCGCTAACTG
TCACCCAACATACCATTAATACTTCGCCGCCTTTCCTTCGGTTAATGGGCACGCCTACGAGCATCCCGAATGATACCTGG
TTACAGGGGCATTCGTTTCTCTCAACCGTAGCAAAATATAGTAATGATCTTCCTCAGGCTGAAATGACAGCCAGAGAGCT
TGCTTATAACGCACCAGGAAATCAGGGACTGCGCATTGATTACGCGAGTGTGTTACAAGCCCGCGGTTGGCCTCGTGCAG
CAGAAAATGAATTAAAAAAAGCAGAAGTGATCGAGCCACGTAATATTAATCTGGAGGTTGAACAAGCCTGGACAGCATTA
ACGTTACAAGAATGGCAGCAGGCAGCTGTCTTAACGCACGATGTTGTCGAACGTGAACCGCAAGATCCCGGCGTTGTACG
ATTAAAACGTGCGGTTGATGTACATAATCTTGCAGAGCTTCGTATCGCTGGCTCAACAGGAATTGATGCCGAAGGCCCGG
ATAGTGGTAAACATGATGTCGACTTAACCACCATCGTTTATTCACCACCGCTGAAGGATAACTGGCGCGGTTTTGCTGGA
TTCGGTTATGCCGATGGACAATTTAGCGAAGTAAAAGGGATTGTTCGCGACTGGCTTGCGGGTGTTGAGTGGCGGTCACG
TAATATCTGGCTCGAGGCAGAGTACGCTGAACGCGTTTTCAATCATGAGCATAAACCCGGCGCGCGCCTGTCTGGCTGGT
ATGATTTTAATGATAACTGGCGTATTGGTTCGCAACTGGAACGCCTCTCTCACCGCGTTCCATTACGGGCAATGAAAAAT
GGTGTTACAGGCAACAGTGCTCAGGCTTATGTTCGCTGGTATCAAAATGAGCGGCGTAAGTACGGTGTCTCCTGGGCTTT
CACTGATTTTTCCGACAGTAACCAGCGTCATGAAGTCTCACTTGAGGGTCAGGAACGCATCTGGTCTTCACCATATTTGA
TTGTCGATTTCCTACCCAGTCTGTATTACGAACAAAATACAGAACACGATACCCCATACTACAACCCTATAAAAACGTTC
GATATTGTTCCGGCATTTGAGGCAAGCCATTTGTTATGGCGAAGCTATGAAAATAGCTGGGAGCAAATATTCAGCGCAGG
TGTTGGTGCCTCCTGGCAAAAACATTATGGCACGGATGTCGTCACCCAACTCGGCTACGGGCAACGCATTAGCTGGAATG
ACGTGATTGATGCTGGCGCAACGCTACGCTGGGAAAAACGACCTTATGACGGTGACAGAGAACACAACTTATACGTTGAA
TTCGATATGACATTCAGATTTTAA

Upstream 100 bases:

>100_bases
GCGTTTAATAACGGATTATGAGGTGCAAAAATATCTTTCTTTTCAGTTACCTGTAATTAGATACAGAGAGAGATTTTGGC
AATACATGGAGTAATACAGG

Downstream 100 bases:

>100_bases
GGATAAATATGTTACGTAATGGAAATAAATATCTCCTGATGCTGGTGAGTATAATTATGCTCACCGCGTGCATTAGCCAG
TCAAGAACATCATTTATACC

Product: outer membrane protein PgaA

Products: NA

Alternate protein names: PGA export protein; Poly-beta-1,6-GlcNAc export protein [H]

Number of amino acids: Translated: 807; Mature: 807

Protein sequence:

>807_residues
MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQ
DKQVITVYNRYRHQQLPARGYAAVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF
MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVA
SAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTW
LQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQAWTAL
TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAG
FGYADGQFSEVKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKN
GVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF
DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVE
FDMTFRF

Sequences:

>Translated_807_residues
MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQ
DKQVITVYNRYRHQQLPARGYAAVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF
MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVA
SAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTW
LQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQAWTAL
TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAG
FGYADGQFSEVKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKN
GVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF
DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVE
FDMTFRF
>Mature_807_residues
MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQ
DKQVITVYNRYRHQQLPARGYAAVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF
MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVA
SAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTW
LQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQAWTAL
TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAG
FGYADGQFSEVKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKN
GVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF
DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVE
FDMTFRF

Specific function: Exports the biofilm adhesin polysaccharide poly-beta- 1,6-N-acetyl-D-glucosamine (PGA) across the outer membrane. The PGA transported seems to be partially N-deacetylated since N- deacetylation of PGA by PgaB is needed for PGA export through the PgaA pori

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI1787261, Length=807, Percent_Identity=99.8760842627014, Blast_Score=1662, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013026
- InterPro:   IPR011990
- InterPro:   IPR019734 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 92250; Mature: 92250

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS50005 TPR ; PS50293 TPR_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALSWFALK
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHH
SALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYAAVAVAYRNLQQWQNSLTL
HHHCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
WQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNSGAPDKANLLAEAYIYKLAGR
HHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
HQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHEE
MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISH
CCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHH
YQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFY
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTWLQGHSFLSTVAKYSNDLPQA
HHHCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHH
EMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQAWTAL
HHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHH
TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDV
HHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCHHHHHHEEEECCCCCCCCCCCCCCCCE
DLTTIVYSPPLKDNWRGFAGFGYADGQFSEVKGIVRDWLAGVEWRSRNIWLEAEYAERVF
EEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECCEEEEEEHHHHHHH
NHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRK
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHH
YGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF
CCCEEEEECCCCCCCCEEEEECHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCE
DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGA
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHCCCCCCCCHHHHHCCCC
TLRWEKRPYDGDREHNLYVEFDMTFRF
EEEECCCCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALSWFALK
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHH
SALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYAAVAVAYRNLQQWQNSLTL
HHHCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
WQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNSGAPDKANLLAEAYIYKLAGR
HHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
HQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSF
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHEE
MPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISH
CCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHH
YQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFY
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTWLQGHSFLSTVAKYSNDLPQA
HHHCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHH
EMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQAWTAL
HHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHH
TLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDV
HHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCHHHHHHEEEECCCCCCCCCCCCCCCCE
DLTTIVYSPPLKDNWRGFAGFGYADGQFSEVKGIVRDWLAGVEWRSRNIWLEAEYAERVF
EEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECCEEEEEEHHHHHHH
NHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRK
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHH
YGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTF
CCCEEEEECCCCCCCCEEEEECHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCE
DIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGA
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHCCCCCCCCHHHHHCCCC
TLRWEKRPYDGDREHNLYVEFDMTFRF
EEEECCCCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]