Click here to switch to the map view.

The map label for this gene is ybiN

Identifier: 218694281

GI number: 218694281

Start: 902565

End: 903491

Strand: Direct

Name: ybiN

Synonym: EC55989_0851

Alternate gene names: 218694281

Gene position: 902565-903491 (Clockwise)

Preceding gene: 218694275

Following gene: 218694288

Centisome position: 17.51

GC content: 53.83

Gene sequence:

>927_bases
ATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGTCAGGTCAA
TCCTGAACTCAGGCAATTCCTCACGCTTACACCCGCAGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGG
CGCTCAATAAGGCATTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCGGACGGTTTTCTCTGCCCGCCAGTA
CCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCTAATGCCAGCAT
TCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTG
AAACCAGCAGTCAGGCGTTAAGTAGTGCGCAGGCGATCATCAGTGCTAATCCGGGGCTTAACCGCGCCATTCGTCTGCGT
CGGCAAAAAGAGAGTGGGGCGATTTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTAACCCGCC
ATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCAC
TGAACTTTGGCGGCCAGCAACAGGAGTTGTGGTGTGAAGGTGGTGAAGTCGCCTTTATCAAAAAGATGATTGAAGAGAGC
AAAGGCTTCGCGAAGCAGGTGATGTGGTTTACCTCGCTGGTTTCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCTCT
GACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCT
TTATGAACGACGAGCAGCGCCGCCGTTTTGTTAATCGCCAACGTTAA

Upstream 100 bases:

>100_bases
CCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCC
GCTGCTTAAAGGAGAATGCT

Downstream 100 bases:

>100_bases
AGCGTTGGCTCCCCCGGCGGCAGCGGTTCAGCCGGGGCCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGC
GCGAACACCTGCCAGGTCGA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSVDFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPV
PGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGGQQQELWCEGGEVAFIKKMIEES
KGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR

Sequences:

>Translated_308_residues
MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSVDFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPV
PGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGGQQQELWCEGGEVAFIKKMIEES
KGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR
>Mature_307_residues
SAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSVDFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVP
GRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLRR
QKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGGQQQELWCEGGEVAFIKKMIEESK
GFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Homo sapiens, GI122114654, Length=293, Percent_Identity=30.7167235494881, Blast_Score=148, Evalue=7e-36,
Organism=Escherichia coli, GI87081786, Length=308, Percent_Identity=99.3506493506494, Blast_Score=640, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32564931, Length=308, Percent_Identity=26.6233766233766, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32564933, Length=308, Percent_Identity=24.6753246753247, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281363415, Length=291, Percent_Identity=29.553264604811, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI19922302, Length=291, Percent_Identity=29.553264604811, Blast_Score=124, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_ECO24 (A7ZJM2)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001462001.1
- ProteinModelPortal:   A7ZJM2
- SMR:   A7ZJM2
- STRING:   A7ZJM2
- EnsemblBacteria:   EBESCT00000022332
- GeneID:   5588793
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0877
- eggNOG:   COG3129
- GeneTree:   EBGT00050000011484
- HOGENOM:   HBG747880
- ProtClustDB:   PRK11727
- BioCyc:   ECOL331111:ECE24377A_0877-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PANTHER:   PTHR13393
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 34180; Mature: 34049

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSVDFANPLAVKALNKALL
CCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHH
AHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPL
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHH
IGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLRRQKESGAIFNGIIHKNEQYD
HHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEHHHHCCCCCCC
ATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGGQQQELWCEGGEVAFIKKMIEES
CEECCCCCCCCHHHHHCCHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHH
KGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEEEECHHHH
RRFVNRQR
HHHHCCCC
>Mature Secondary Structure 
SAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSVDFANPLAVKALNKALL
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHH
AHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPL
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHH
IGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLRRQKESGAIFNGIIHKNEQYD
HHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEHHHHCCCCCCC
ATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGGQQQELWCEGGEVAFIKKMIEES
CEECCCCCCCCHHHHHCCHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHH
KGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEEEECHHHH
RRFVNRQR
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA