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The map label for this gene is ybeM

Identifier: 218694066

GI number: 218694066

Start: 673703

End: 674266

Strand: Direct

Name: ybeM

Synonym: EC55989_0618

Alternate gene names: 218694066

Gene position: 673703-674266 (Clockwise)

Preceding gene: 218694064

Following gene: 218694067

Centisome position: 13.07

GC content: 54.61

Gene sequence:

>564_bases
ATGATGACGACAATTCTGACGATTCATGTTCCTTCAACGCCGGGGCGCGCATGGAATATGCTGGTGGCGCTACAGGCAGG
AAACATCGTCGCCCGTTATGCCAAATTGCATCTCTATGATGCATTTGCCATTCAGGAATCACGCCGTGTTGATGCTGGTA
ATGAAATCGCTCCGTTACTCGAGGTGGAGGGGATGAAGGTCGGTCTGATGACCTGTTATGACTTACGCTTTCCAGAGCTG
GCGCTGGCACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCA
CTGGTCAACGTTGCTTGCCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCTGCGGGGGAGTGCGGGAACAAAAATA
TCGGTCAAAGCCGGATTATAGATCCCTTTGGCGTCACCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAA
GTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATT
ATGA

Upstream 100 bases:

>100_bases
GCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACGTTTAC
GGCGAGAAAGTAAACGTAAC

Downstream 100 bases:

>100_bases
TGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCT
GGTTTTTAAGGCGCGTTCTG

Product: putative amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MMTTILTIHVPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPEL
ALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAE
VTPERVRQVRAQLPVLNNRRFAPPQLL

Sequences:

>Translated_187_residues
MMTTILTIHVPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPEL
ALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAE
VTPERVRQVRAQLPVLNNRRFAPPQLL
>Mature_187_residues
MMTTILTIHVPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPEL
ALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAE
VTPERVRQVRAQLPVLNNRRFAPPQLL

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain

Homologues:

Organism=Homo sapiens, GI5031947, Length=178, Percent_Identity=39.8876404494382, Blast_Score=121, Evalue=5e-28,
Organism=Homo sapiens, GI297632350, Length=178, Percent_Identity=39.8876404494382, Blast_Score=120, Evalue=6e-28,
Organism=Homo sapiens, GI297632348, Length=178, Percent_Identity=39.8876404494382, Blast_Score=120, Evalue=6e-28,
Organism=Homo sapiens, GI9910460, Length=179, Percent_Identity=29.0502793296089, Blast_Score=91, Evalue=5e-19,
Organism=Escherichia coli, GI1786412, Length=166, Percent_Identity=29.5180722891566, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556280, Length=181, Percent_Identity=35.3591160220994, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322335, Length=197, Percent_Identity=33.502538071066, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323383, Length=183, Percent_Identity=31.1475409836066, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21355835, Length=167, Percent_Identity=32.3353293413174, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17933642, Length=166, Percent_Identity=33.7349397590361, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YBEM_ECO57 (P58054)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85562
- PIR:   H90711
- RefSeq:   NP_286352.1
- RefSeq:   NP_308691.1
- ProteinModelPortal:   P58054
- SMR:   P58054
- EnsemblBacteria:   EBESCT00000028488
- EnsemblBacteria:   EBESCT00000059185
- GeneID:   917024
- GeneID:   957658
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0771
- KEGG:   ecs:ECs0664
- GeneTree:   EBGT00050000010760
- HOGENOM:   HBG742418
- OMA:   VWEKNAQ
- BioCyc:   ECOL83334:ECS0664-MONOMER
- InterPro:   IPR003010
- InterPro:   IPR001110
- Gene3D:   G3DSA:3.60.110.10

Pfam domain/function: PF00795 CN_hydrolase; SSF56317 Ntlse/CNhydtse

EC number: 3.5.-.- [C]

Molecular weight: Translated: 20482; Mature: 20482

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS50263 CN_HYDROLASE; PS01227 UPF0012

Important sites: ACT_SITE 40-40 ACT_SITE 110-110 ACT_SITE 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTILTIHVPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRRVDAGNEIAPLL
CCEEEEEEEECCCCCCCEEEEEEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
EVEGMKVGLMTCYDLRFPELALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTT
EECCCEEEEEEEECCCCCHHHHHHHHCCCEEEEECHHHHCCCHHHHHHHHHHHHHHHCCE
CYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRR
EEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCHHHHHHHHHHCCCCCCCC
FAPPQLL
CCCCCCC
>Mature Secondary Structure
MMTTILTIHVPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRRVDAGNEIAPLL
CCEEEEEEEECCCCCCCEEEEEEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
EVEGMKVGLMTCYDLRFPELALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTT
EECCCEEEEEEEECCCCCHHHHHHHHCCCEEEEECHHHHCCCHHHHHHHHHHHHHHHCCE
CYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRR
EEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCHHHHHHHHHHCCCCCCCC
FAPPQLL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796