Click here to switch to the map view.

The map label for this gene is cusS

Identifier: 218694010

GI number: 218694010

Start: 609880

End: 611340

Strand: Reverse

Name: cusS

Synonym: EC55989_0561

Alternate gene names: 218694010

Gene position: 611340-609880 (Counterclockwise)

Preceding gene: 218694011

Following gene: 218694009

Centisome position: 11.86

GC content: 51.88

Gene sequence:

>1461_bases
TTGAGGTGCCGGATGGTCAGTAAGCCATTTCAGCGCCCGTTTTCGCTGGCAACCCGCCTGACCTTTTTTATCAGCCTGGC
CACCATCGCAGCGTTTTTCGCCTTTGCATGGATCATGATCCACTCAGTAAAAGTGCATTTTGCCGAGCAGGATATTAATG
ATTTAAAAGAAATTAGCGCCACGCTTGAACGGGTACTTAATCATCCTGACGAGACGCAAGCCCGACGCTTAATGACGCTG
GAAGATATCGTCAGTGGTTATTCCAACGTGTTGATCTCCCTGGCAGATAGCCATGGTAAAACGGTGTATCACTCCCCCGG
TGCGCCGGATATCCGCGAGTTTACGCGTGACGCCATACCCGATAAAGACGCGCGGGGCGGCGAGGTGTATCTCCTTTCCG
GCCCGACGATTATGATGCCAGGGCATGGTCACGGGCATATGGAACACAGCAACTGGCGGATGATTAACTTGTCGGTTGGC
CCGTTGGTGGACGGCAAACCGATTTATACGCTCTACATCGCGCTTTCGATCGATTTTCATCTTCATTACATAAATGATTT
GATGAATAAACTTATTATGACCGCATCGATCATCAGCATCCTGATCGTCTTTATCGTGCTATTGGCGGTACATAAAGGTC
ACGCGCCGATCCGCAGCGTCAGCCGTCAAATCCAGAATATTACCTCGAAAGATCTCGACGTTCGCCTCGACCCGCAGACC
GTGCCCATTGAGCTGGAACAGCTGGTACTGTCGTTCAACCATATGATCGAGCGTATTGAGGATGTCTTTACCCGCCAGTC
CAATTTCTCAGCGGATATCGCTCACGAAATTCGCACACCAATTACTAATCTCATAACGCAAACGGAAATCGCCCTCAGCC
AGTCGCGCAGCCAGAAGGAACTGGAAGATGTGCTCTACTCTAATCTCGAAGAGCTGACGCGAATGGCGAAAATGGTCAGC
GATATGCTGTTTCTCGCTCAGGCCGATAACAACCAGCTAATCCCCGAAAAGAAAATGCTCAACCTGGCGGATGAAGTCGG
CAAAGTGTTCGATTTTTTCGAGGCGTTAGCGGAAGATCGCGGCGTGGAGTTGCGGTTTGTTGGCGACAAATGTCAGGTTG
CGGGCGATCCGCTGATGCTGCGTCGGGCGTTAAGCAACCTGCTTTCTAATGCCCTGCGTTATACGCCACCCAGTGAGGCT
ATTGTAGTGCGCTGCCAGACGGTCAATCATCAGGTGCAAGTTTCCGTCGAAAACCCCGGTACGCCCATTGCGCCCGAGCA
CTTACCGCGATTGTTTGACCGTTTCTATCGCGTTGACCCTTCCCGCCAGCGAAAAGGTGAAGGTAGCGGTATTGGGCTGG
CGATAGTGAAATCGATTGTTGTCGCGCATAAAGGCACGGTTGCGGTAACGTCAGATGCGCGGGGGACAAGGTTTGTGATT
ATGCTGCCGGAGAGAGAGTGA

Upstream 100 bases:

>100_bases
ACTAACGCCATTGATGTAGCGGTGAAGCGGCTGCGCGGCAAAATCGACAACGACTTTGAGCCAAAGCTAATTCAGACCGT
GCGCGGCGTGGGTTACATGC

Downstream 100 bases:

>100_bases
TTATTAAGTTCAAATTCAGGGCGTGGTGCATCACCTCCTGGTATTGTTGATCAACAAATTAGAGAAAAACTTATTAAACA
TATGAAATATCGAAGGCTTT

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MRCRMVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTL
EDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFTRDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVG
PLVDGKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
VPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVS
DMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPSEA
IVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
MLPERE

Sequences:

>Translated_486_residues
MRCRMVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTL
EDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFTRDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVG
PLVDGKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
VPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVS
DMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPSEA
IVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
MLPERE
>Mature_486_residues
MRCRMVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTL
EDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFTRDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVG
PLVDGKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
VPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVS
DMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPSEA
IVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
MLPERE

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=479, Percent_Identity=97.4947807933194, Blast_Score=943, Evalue=0.0,
Organism=Escherichia coli, GI1788279, Length=296, Percent_Identity=33.445945945946, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1786600, Length=265, Percent_Identity=33.9622641509434, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790346, Length=332, Percent_Identity=29.2168674698795, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788393, Length=275, Percent_Identity=29.8181818181818, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787894, Length=311, Percent_Identity=29.2604501607717, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789403, Length=282, Percent_Identity=30.8510638297872, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI145693157, Length=237, Percent_Identity=26.5822784810127, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789808, Length=283, Percent_Identity=27.5618374558304, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=27.5423728813559, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=26.3636363636364, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790551, Length=296, Percent_Identity=27.3648648648649, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081816, Length=253, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788713, Length=232, Percent_Identity=28.0172413793103, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790861, Length=298, Percent_Identity=25.503355704698, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87082128, Length=295, Percent_Identity=24.7457627118644, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787374, Length=340, Percent_Identity=22.0588235294118, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786912, Length=244, Percent_Identity=25.4098360655738, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI48994928, Length=237, Percent_Identity=24.0506329113924, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788549, Length=223, Percent_Identity=26.0089686098655, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54666; Mature: 54666

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCRMVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHH
TLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFTRDAIP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHCCC
DKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVDGKPIYTLYIALSIDFH
CCCCCCCEEEEEECCEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEEEEEEHH
LHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCC
VPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPSEAIVVRCQTVNHQVQVSVENPG
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEECCCC
TPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
CCCCHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEE
MLPERE
EECCCC
>Mature Secondary Structure
MRCRMVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHH
TLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFTRDAIP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHCCC
DKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVDGKPIYTLYIALSIDFH
CCCCCCCEEEEEECCEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEEEEEEHH
LHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCC
VPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPSEAIVVRCQTVNHQVQVSVENPG
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEECCCC
TPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
CCCCHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEE
MLPERE
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]