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The map label for this gene is glxR

Identifier: 218693973

GI number: 218693973

Start: 564480

End: 565358

Strand: Direct

Name: glxR

Synonym: EC55989_0524

Alternate gene names: 218693973

Gene position: 564480-565358 (Clockwise)

Preceding gene: 218693972

Following gene: 218693974

Centisome position: 10.95

GC content: 50.74

Gene sequence:

>879_bases
ATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACA
TGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAGGTAACGG
AAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCTCAGGTTGAAGAAGTTCTGTTCGGTGAAAATGGTTGTACC
AAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGT
GAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTA
TGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGC
GGTAACGGCGACGGTCAAACCTGCAAAGTGGCAAATCAGATTATTGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCT
GCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGATGGGCGGCTTTGCTTCCTCACGTATTC
TGGAAGTTCATGGCGAGCGTATGATTAAACGCACCTTTAATCCGGGCTTCAAAATCGCTCTGCACCAGAAAGATCTCAAC
CTGGCACTGCAAAGTGCGAAAGCACTTGCGCTGAACCTGCCAAACACTGCGACCTGCCAGGAGTTATTTAATACCTGTGC
GGCAAACGGTGGCAGCCAGTTGGATCACTCTGCGTTAGTGCAGGCGCTGGAATTAATGGCTAACCATAAACTGGCCTGA

Upstream 100 bases:

>100_bases
AAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCG
AAATTTAAAAGAGGTTAATT

Downstream 100 bases:

>100_bases
TACCCGCAATAAAAATGGCCGATATCAGAAAATGAATCGGCCAGCAATATTAAAAAAGAAAGCAGCCAAAGATGTTGCTT
CAGTATTAAAAATAATATTT

Product: tartronate semialdehyde reductase, NADH-dependent

Products: NA

Alternate protein names: Tartronate semialdehyde reductase; TSAR

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAVSVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCT
KASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN
LALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELMANHKLA

Sequences:

>Translated_292_residues
MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAVSVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCT
KASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN
LALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELMANHKLA
>Mature_292_residues
MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAVSVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCT
KASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN
LALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELMANHKLA

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI23308751, Length=300, Percent_Identity=32, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI40556376, Length=283, Percent_Identity=27.5618374558304, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786719, Length=292, Percent_Identity=100, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI145693186, Length=289, Percent_Identity=43.9446366782007, Blast_Score=240, Evalue=7e-65,
Organism=Escherichia coli, GI1790315, Length=289, Percent_Identity=31.1418685121107, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789092, Length=286, Percent_Identity=28.3216783216783, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17557316, Length=257, Percent_Identity=33.0739299610895, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24655230, Length=300, Percent_Identity=32.6666666666667, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922568, Length=300, Percent_Identity=32.6666666666667, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=30.3571428571429, Blast_Score=117, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLXR_ECOLI (P77161)

Other databases:

- EMBL:   U89279
- EMBL:   U82664
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D64782
- RefSeq:   AP_001157.1
- RefSeq:   NP_415042.1
- ProteinModelPortal:   P77161
- SMR:   P77161
- STRING:   P77161
- EnsemblBacteria:   EBESCT00000000618
- EnsemblBacteria:   EBESCT00000015201
- GeneID:   945146
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0497
- KEGG:   eco:b0509
- EchoBASE:   EB3052
- EcoGene:   EG13265
- eggNOG:   COG2084
- GeneTree:   EBGT00050000009837
- HOGENOM:   HBG729179
- OMA:   ALPNTAT
- ProtClustDB:   PRK15059
- BioCyc:   EcoCyc:G6278-MONOMER
- BioCyc:   MetaCyc:G6278-MONOMER
- Genevestigator:   P77161
- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183
- InterPro:   IPR006398
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR22981
- PRINTS:   PR00076
- TIGRFAMs:   TIGR01505

Pfam domain/function: PF03446 NAD_binding_2; SSF48179 6DGDH_C_like

EC number: =1.1.1.60

Molecular weight: Translated: 30801; Mature: 30801

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: ACT_SITE 169-169 BINDING 94-94 BINDING 237-237

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAVSVETARQVTEASDIIF
CCEEEEECCCCCCCCEEEEECCCCEEEEEEECHHHHHHHHHCCHHHHHHHHHHCCCCEEE
IMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVS
EEECCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEECCCCC
GGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVGGNGDGQTCKVANQIIVALNI
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCEEEEEEEH
EAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHCCCCEEEEEEECCHH
LALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELMANHKLA
HHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAVSVETARQVTEASDIIF
CCEEEEECCCCCCCCEEEEECCCCEEEEEEECHHHHHHHHHCCHHHHHHHHHHCCCCEEE
IMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVS
EEECCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEECCCCC
GGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVGGNGDGQTCKVANQIIVALNI
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCEEEEEEEH
EAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHCCCCEEEEEEECCHH
LALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELMANHKLA
HHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10601204; 9278503