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The map label for this gene is hsdM [H]

Identifier: 218692732

GI number: 218692732

Start: 5144909

End: 5146414

Strand: Reverse

Name: hsdM [H]

Synonym: ECED1_5213

Alternate gene names: 218692732

Gene position: 5146414-5144909 (Counterclockwise)

Preceding gene: 218692733

Following gene: 218692731

Centisome position: 98.79

GC content: 50.0

Gene sequence:

>1506_bases
ATGTTGCAGAAGGCCTTGTTTTTCATGGAATACAACATGTCTATCAGTTCAGTAATCAAATCATTACAAGATATTATGCG
CAAAGATGCCGGTGTGGATGGCGATGCGCAGCGTCTCGGTCAGCTCTCCTGGCTGCTGTTTTTGAAAATCTTCGATGCCC
AGGAAGAGGCGCTGGAACTGGAGCAGGATAACTATCAATATCCGATCCCACAGCGTTATTTATGGCGCAGTTGGGCCGCA
AACGCTCAGGGCATTACCGGTGATTCCCTGCTGGAATTCGTTAATGATGATCTGTTCCCGGCGTTGAAAAACCTCACTGC
GCCTATCGATAAAAACCCACGCGGCTACGTGGTGAAGCAGGCGTTCAGCGATGCCTATAACTATATGAAAAACGGTACGC
TACTGCGCCAGGTGATCAACAAGCTGAACGAAATTGACTTTACCAGCGCCAGCGAACGCCATCTGTTTGGTGATATTTAC
GAACAGATCCTTAAAGATCTGCAATCTGCGGGCAATGCTGGCGAATTCTATACTCCACGCGCCGTCACTCGCTTTATGGT
GGATCGCGTTGATCCGAAACTCGGCGAATCCATTATGGACCCGGCCTGCGGTACGGGCGGTTTTCTTGCCTGCGCGTTCG
ATCATGTAAAGAACAAATACGTGAAGAGCGTCGCCGATCATCAGACGCTGCAACAACAGATCCACGGTGTTGAGAAAAAA
CAGCTTCCGCACCTGCTGGCGACCACCAATATGCTGCTGCACGGCATTGAAGTACCGGTACAAATTCGTCACGACAACAC
CCTGAACAAGCCGCTTTCCTCCTGGGATGAGCAACTGGATGTCATTGTTACCAACCCGCCATTTGGTGGCACGGAAGAAG
ACGGTATTGAGAAGAACTTTCCGGCAGAGATGCAAACCCGCGAAACGGCGGATCTGTTCCTGCAACTGATTGTGGAAGTA
CTGGCGAAAAACGGTCGTGCGGCGGTGGTATTGCCGGATGGCACGCTATTTGGCGAAGGCGTTAAAACCAAAATCAAAAA
GCTGCTTACCGAAGAGTGCAATCTGCATACCATCGTGCGTTTACCGAATGGGGTGTTTAACCCCTATACCGGCATTAAAA
CCAACCTGTTGTTCTTTACCAAAGGTCAGCCAACCAAAGAGATTTGGTTCTATGAGCATCCGTATCCGGCGGGCGTGAAA
AATTACAGTAAAACCAAGCCGATGAAGTTTGAAGAGTTTCAGGCGGAAATCGACTGGTGGGGTAACGAGGCCGATGGTTT
TGCCAGCCGCGTCGAGAATGAGCAGGCATGGAAAGTCAGCATTGATGACGTGATTGCGCGTAACTTCAATCTGGATATTA
AAAACCCACATCAGGCGGAAACCGTTAGCCATGATCCGGACGAACTGTTAGCGCAGTATGCAAAACAGCAGGCGGAGATC
CAGACGCTGCGTAATCAACTGTGCGATATTCTTGGCGCTGCGCTGTCTGGCAAGGAGGTTAACTAA

Upstream 100 bases:

>100_bases
TAACGGGTATAATCAGGCGCTCAGCGAGCTGGAAAACGAAATCTACCAATTACCGCCCCGCTCTGCTTAACAACTCTCTT
TTGCGCCTTCTGCAACCATC

Downstream 100 bases:

>100_bases
TGAGTGTGGAAAAGCTGATCGTTGATCATATGGAAACCTGGACCTCGGCGTTGCAAACCCGTTCCACCGCCGGGCGCGGC
AGTTCCGGTAAAATTGATTT

Product: Type I restriction enzyme EcoEI M protein (M.EcoEI)

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAA
NAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTLQQQIHGVEKK
QLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEV
LAKNGRAAVVLPDGTLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVK
NYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETVSHDPDELLAQYAKQQAEI
QTLRNQLCDILGAALSGKEVN

Sequences:

>Translated_501_residues
MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAA
NAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTLQQQIHGVEKK
QLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEV
LAKNGRAAVVLPDGTLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVK
NYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETVSHDPDELLAQYAKQQAEI
QTLRNQLCDILGAALSGKEVN
>Mature_501_residues
MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAA
NAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTLQQQIHGVEKK
QLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEV
LAKNGRAAVVLPDGTLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVK
NYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETVSHDPDELLAQYAKQQAEI
QTLRNQLCDILGAALSGKEVN

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=423, Percent_Identity=29.0780141843972, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 56694; Mature: 56694

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]