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The map label for this gene is msbB [H]

Identifier: 218692669

GI number: 218692669

Start: 5076938

End: 5077882

Strand: Reverse

Name: msbB [H]

Synonym: ECED1_5141

Alternate gene names: 218692669

Gene position: 5077882-5076938 (Counterclockwise)

Preceding gene: 218692670

Following gene: 218692668

Centisome position: 97.47

GC content: 42.75

Gene sequence:

>945_bases
ATGAAAAAATATAAATCTGAGTTTATTCCTGAATTTAAGAAAAATTACCTTTCCCCTGTTTACTGGTCTACATGGTTCCT
TTTGGGAATGATTGCAGGTATTTCAATGTTTCCCCCTTCATTCAGAGATCCTGTCTTGGCAAAAATAGGGCGTTGGGCGG
GCAGATTGAGCAAAAAAGCTCGTCGCAGGGCGACGATTAATTTATCGCTTTGTTTCCCAGAAAAGAATGATACAGAACGG
GAAATAATTGTCGACAAAATGTTTGCCACAGCATTGCAATCCATAGTGATGATGGCAGAACTGGCAATTCGTGGTCCGGA
AAAGTTCCAGAAACGAGTGTTCTGGAAAGGCCTCGAAATTCTTGAGGAAATTCGACACAATAATAGAAATGTGATTTTTC
TGGTTCCCCATGGCTGGAGCGTGGATATTCCTGCAATGTTGCTGGCAGCTCAGGGAAAAAAAATGGCCGCCATGTTTCAT
CAGCAACGAAATCCAGTGATTGATTATGTCTGGAATTCAGTACGGCGTAAATTCGGGGGGCGCTTACATGCCCGGGAGGA
TGGGATAAAACCATTTATTCAGTCAGTACGCCAGGGATACTGGGGATATTACCTCCCAGATCAGGATCATGGTCCTGAAT
ACAGTGAATTTGCTGATTTTTTTGCGACCTATAAAGCGACATTACCAATCATTGGACGTCTGATGAACATCAGTCAGGCT
ATGATTATACCACTTTTCCCGGTTTATGATGAAAAAAAACATCTCCTGACTATTGAAATTCGGCCACCAATGGATGCTTG
CATTGCCAGCGCGGACAATAAAATGATTGCCCGACAAATGAACAAAACAGTGGAAATTTTGGTGGGGCCACATCCGGAAC
AGTATGTCTGGGTTTTAAAATTGTTAAAAACACGCAAATCAAACGAAGCGGACCCGTACCCTTGA

Upstream 100 bases:

>100_bases
TATCCTGTACACTCAGAACATCAGAATTTAAATTAAGGTTTGATGTATCCTCTGAATAAAACAGTAATGATAATTTTGGT
AGAGGCATTCGCACTAAATA

Downstream 100 bases:

>100_bases
AATTATAAAGTACAAGTATTTCCACTAGTTGTTTGTAAGCGTACAGCCTGAACCGTCTGGTCAGAATCTGACGAATTAGA
CAAAGTGGTGTCCACCAAAT

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: KDO2-lipid A, cold adapted; [acyl carrier protein]; KDO2-lipid A [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDPVLAKIGRWAGRLSKKARRRATINLSLCFPEKNDTER
EIIVDKMFATALQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQA
MIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKMIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP

Sequences:

>Translated_314_residues
MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDPVLAKIGRWAGRLSKKARRRATINLSLCFPEKNDTER
EIIVDKMFATALQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQA
MIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKMIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP
>Mature_314_residues
MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDPVLAKIGRWAGRLSKKARRRATINLSLCFPEKNDTER
EIIVDKMFATALQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQA
MIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKMIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=312, Percent_Identity=70.1923076923077, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1787292, Length=318, Percent_Identity=28.9308176100629, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082094, Length=308, Percent_Identity=26.6233766233766, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 36482; Mature: 36482

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDPVLAKIGRWAGRLSKKA
CCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
RRRATINLSLCFPEKNDTEREIIVDKMFATALQSIVMMAELAIRGPEKFQKRVFWKGLEI
HHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFHQQRNPVIDYVWNSVRRKFGG
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
RLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQA
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKMIARQMNKTVEILVGPHPEQYVWVLK
HHHCCCCCCCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHCCEEEEEECCCCHHHHHHHH
LLKTRKSNEADPYP
HHHHHCCCCCCCCC
>Mature Secondary Structure
MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDPVLAKIGRWAGRLSKKA
CCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
RRRATINLSLCFPEKNDTEREIIVDKMFATALQSIVMMAELAIRGPEKFQKRVFWKGLEI
HHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFHQQRNPVIDYVWNSVRRKFGG
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
RLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQA
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKMIARQMNKTVEILVGPHPEQYVWVLK
HHHCCCCCCCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHCCEEEEEECCCCHHHHHHHH
LLKTRKSNEADPYP
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-(palmitoleoyl)-lipid IVA; myristoyl-ACP; KDO2-(lauroyl)-lipid IVA [C]

Specific reaction: KDO2-(palmitoleoyl)-lipid IVA + myristoyl-ACP = KDO2-lipid A, cold adapted + [acyl carrier protein] KDO2-(lauroyl)-lipid IVA + myristoyl-ACP = KDO2-lipid A + [acyl carrier protein] [C]

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11115111; 11292750; 12384590; 9171415 [H]