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The map label for this gene is insH [H]

Identifier: 218692660

GI number: 218692660

Start: 5069061

End: 5070077

Strand: Reverse

Name: insH [H]

Synonym: ECED1_5129

Alternate gene names: 218692660

Gene position: 5070077-5069061 (Counterclockwise)

Preceding gene: 218692663

Following gene: 218692656

Centisome position: 97.32

GC content: 54.57

Gene sequence:

>1017_bases
ATGTTTGTCATCTGGAGCCATGGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAA
GCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCG
AGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCTGGAAACCATGCTACGCATTCACTGCATGCAGCAT
TGGTACAACCTGAGCGATGGCGCGATGGAAGATGCTCTGTACGAAATCGCCTCCATGCGTCTGTTTGCCCGGTTATCCCT
GGATAGCGCCTTGCCTGACCGCACCACCATCATGAATTTCCGCCACCTGCTGGAGCAGCATCAACTGGCCCGCCAATTGT
TCAAGACCATCAATCGCTGGCTGGCCGAAGCAGGCGTCATGATGACTCAAGGCACCTTGGTCGATGCCACCATCATTGAG
GCACCCAGCTCGACCAAGAACAAAGAGCAGCAACGCGATCCGGAGATGCATCAGACCAAGAAAGGCAATCAGTGGCACTT
TGGCATGAAGGCCCACATTGGTGTCGATGCCAAGAGTGGCCTGACCCACAGCCTAGTCACCACCGCGGCCAACGAGCATG
ACCTCAATCAGCTGGGTAATCTGCTGCATGGAGAGGAGCAATTTGTCTCAGCCGATGCCGGCTACCAAGGGGCGCCACAG
CGCGAGGAGCTGGCCGAGGTGGATGTGGACTGGCTGATCGCCGAGCGCCCCGGCAAGGTAAGAACCTTGAAACAGCATCC
ACGCAAGAGCAAAACGGCCATCAACATCGAATACATGAAAGCCAGCATCCGGGCCAAGGTGGAGCACCCATTTCGCATCA
TCAAGCGACAGTTCGGCTTCGTGAAAGCCAGATACAAGGGGTTGCTGAAAAACGATAACCAACTGGCGATGTTATTCACG
CTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAA

Upstream 100 bases:

>100_bases
GAAACCGAGAGAGAACGAATGAACGCCCTGAGAATGGTCATTCCGCCACGATATCCCTGGTCTCTGATGGAAGGTGCGAA
CAAGTCCCTGATATGAGATC

Downstream 100 bases:

>100_bases
AAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGC
TCAAACATGAAGAAATGAAA

Product: IS5 transposase and trans-activator; CP4-44 prophage

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQH
WYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQ
REELAEVDVDWLIAERPGKVRTLKQHPRKSKTAINIEYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFT
LANLFRADQMIRQWERSH

Sequences:

>Translated_338_residues
MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQH
WYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQ
REELAEVDVDWLIAERPGKVRTLKQHPRKSKTAINIEYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFT
LANLFRADQMIRQWERSH
>Mature_338_residues
MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQH
WYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQ
REELAEVDVDWLIAERPGKVRTLKQHPRKSKTAINIEYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFT
LANLFRADQMIRQWERSH

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1788303, Length=338, Percent_Identity=99.7041420118343, Blast_Score=707, Evalue=0.0,
Organism=Escherichia coli, GI1789921, Length=338, Percent_Identity=99.1124260355029, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1789612, Length=338, Percent_Identity=99.1124260355029, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1789355, Length=338, Percent_Identity=99.1124260355029, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1788518, Length=338, Percent_Identity=99.1124260355029, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1788342, Length=338, Percent_Identity=99.1124260355029, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1787592, Length=338, Percent_Identity=99.1124260355029, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1786877, Length=338, Percent_Identity=99.1124260355029, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1786764, Length=338, Percent_Identity=99.1124260355029, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1786453, Length=338, Percent_Identity=99.1124260355029, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1787634, Length=325, Percent_Identity=97.5384615384615, Blast_Score=667, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 39174; Mature: 39174

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFYPKA
CEEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
GNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTTIMNF
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPEMHQTK
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEHEEEECCCCCCCCHHHCCCHHHHCC
KGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQ
CCCCEECCEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCC
REELAEVDVDWLIAERPGKVRTLKQHPRKSKTAINIEYMKASIRAKVEHPFRIIKRQFGF
HHHHHHCCHHEEEECCCCCCHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFYPKA
CEEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
GNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTTIMNF
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPEMHQTK
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEHEEEECCCCCCCCHHHCCCHHHHCC
KGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQ
CCCCEECCEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCC
REELAEVDVDWLIAERPGKVRTLKQHPRKSKTAINIEYMKASIRAKVEHPFRIIKRQFGF
HHHHHHCCHHEEEECCCCCCHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]