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The map label for this gene is cpdB

Identifier: 218692605

GI number: 218692605

Start: 5002711

End: 5004654

Strand: Reverse

Name: cpdB

Synonym: ECED1_5072

Alternate gene names: 218692605

Gene position: 5004654-5002711 (Counterclockwise)

Preceding gene: 218692607

Following gene: 218692603

Centisome position: 96.07

GC content: 49.69

Gene sequence:

>1944_bases
ATGATTAAGTTTAGCGCAACGCTCCTAGCCACGCTGATTGCCGCCAGTGTGAATGCAGCGACGGTCGATCTGCGTATCAT
GGAAACCACTGATCTGCATAGCAACATGATGGATTTCGATTATTACAAAGACACCGCCACGGAAAAATTCGGACTGGTAC
GTACGGCAAGCCTGATTAACGATGCCCGCAATGAAGTGAAAAACAGCGTACTGGTCGATAACGGCGATTTGATTCAGGGG
AGTCCGCTGGCAGATTACATGTCGGCGAAAGGATTAAAAGCAGGTGATATTCACCCGGTCTATAAGGCATTAAATACGCT
GGACTATACCGTCGGCACGCTTGGCAACCACGAGTTTAACTACGGTCTGGATTATCTGAAAAATGCGCTGGCGGGAGCGA
AATTCCCTTATGTAAATGCCAACGTCATTGACGCCAGAACCAAACAGCCAATGTTTACACCGTATTTAATTAAAGACACC
GAAGTGGTCGATAAAGACGGAAAAAAACAGACGCTGAAGATTGGTTATATTGGCGTCGTCCCGCCACAAATCATGGGCTG
GGATAAAGCTAATTTATCCGGGAAAGTGACGGTGAATGATATTACCGAAACCGTGCGCAAATACGTGCCTGAAATGCGCG
AGAAAGGTGCCGATGTCGTTGTCGTTCTGGCGCATTCCGGGCTGTCTGCCGATCCGTATAAAGTGATGGCGGAAAACTCA
GTTTATTACCTCAGTGAAATTCCGGGCGTTAACGCCATTATGTTTGGCCATGCTCACGCCGTTTTCCCAGGTAAAGATTT
TGCTGATATCGAAGGGGCTGATATCGCGAAAGGCACGCTGAATGGTGTTCCGGCGGTAATGCCAGGCATGTGGGGCGATC
ATCTTGGTGTGGTCGACTTACAACTCAGTAATGACAGCGGTAAATGGCAGGTGACGCAGGCGAAAGCGGAAGCACGGCCG
ATTTACGACATCGCGAATAAAAAATCCCTCGCGGCGGAAGACAGCAAGCTGGTAGAAACACTCAAAGCCGATCACGATGC
CACACGCCAGTTCGTCAGCAAGCCAATCGGTAAATCTGCCGACAATATGTATAGCTATCTGGCGCTTGTGCAGGACGATC
CGACCGTGCAGGTGGTGAACAACGCGCAAAAAGCGTATGTCGAGCATTACATTCAGGGCGATCCGGATCTGGCAAAACTG
CCAGTGCTTTCAGCTGCCGCACCGTTTAAAGTTGGTGGTCGCAAAAATGACCCGGCAAGCTATGTGGAGGTGGAAAAAGG
CCAGTTGACCTTCCGTAATGCCGCCGATCTTTATCTCTACCCCAATACGCTGATTGTGGTGAAAGCCAGCGGTAAAGAGG
TGAAAGAGTGGCTGGAGTGCTCCGCCGGACAGTTTAACCAGATTGATCCTAACAGCACGAAACCACAGTCACTCATCAAC
TGGGATGGTTTCCGCACCTATAACTTTGATGTTATTGATGGTGTGAATTATCAGATTGATGTTACCCAGCCCGCCCGTTA
TGACGGCGAGTGCCAGATGATTAATGCCAATGCGGAAAGGATTAAGAACCTGACCTTTAACGGCAAGCCGATTGATCCGA
ACGCCATGTTCCTCGTTGCCACCAATAACTATCGCGCTTACGGCGGCAAATTTGCCGGTACGGGCGACAGCCATATCGCT
TTTGCTTCACCGGATGAGAACCGCTCGGTGCTGGCAGCGTGGATTGCTGATGAGTCGAAACGTGCGGGGGAAATTCACCC
GGCGGCAGATAACAACTGGCGTTTAGCACCAATAGCTGGCGATAAGAAACTGGATATCCGTTTCGAAACCTCTCCGTCAG
ATAAAGCCGCAGCGTTTATTAAAGAGAAAGGGCAGTATCCGATGAATAAAGTCGCGACCGATGATATCGGGTTTGCGATT
TATCAGGTGGATCTGAGTAAGTAA

Upstream 100 bases:

>100_bases
ATTTTCAAAGCGTAAAATTGTGGCATTCTTCACTGTTCTATAAGTAAGACGTTTATTCTCCCTTTTCTTTCGTATTCCCG
ATGATAAAAGGATGTCCCTG

Downstream 100 bases:

>100_bases
AACACTTCTTTTTCGGCCTACAAATCATCAACCGCATCCGGCATTTATTGGCGGATGCGATGCTGACGCATCTTATCCGC
CCTACAAGCCATGAACCGTA

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDTATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQG
SPLADYMSAKGLKAGDIHPVYKALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGADVVVVLAHSGLSADPYKVMAENS
VYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIAKGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARP
IYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKL
PVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQSLIN
WDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIA
FASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAI
YQVDLSK

Sequences:

>Translated_647_residues
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDTATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQG
SPLADYMSAKGLKAGDIHPVYKALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGADVVVVLAHSGLSADPYKVMAENS
VYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIAKGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARP
IYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKL
PVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQSLIN
WDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIA
FASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAI
YQVDLSK
>Mature_647_residues
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDTATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQG
SPLADYMSAKGLKAGDIHPVYKALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGADVVVVLAHSGLSADPYKVMAENS
VYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIAKGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARP
IYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKL
PVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQSLIN
WDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIA
FASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAI
YQVDLSK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family

Homologues:

Organism=Escherichia coli, GI1790658, Length=647, Percent_Identity=100, Blast_Score=1329, Evalue=0.0,
Organism=Escherichia coli, GI1786687, Length=576, Percent_Identity=22.5694444444444, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): CPDB_ECOLI (P08331)

Other databases:

- EMBL:   M13464
- EMBL:   U14003
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   X54008
- PIR:   H65232
- RefSeq:   AP_004713.1
- RefSeq:   NP_418634.1
- ProteinModelPortal:   P08331
- SMR:   P08331
- DIP:   DIP-9311N
- MINT:   MINT-1228170
- STRING:   P08331
- EnsemblBacteria:   EBESCT00000003401
- EnsemblBacteria:   EBESCT00000014549
- GeneID:   948729
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW4171
- KEGG:   eco:b4213
- EchoBASE:   EB0158
- EcoGene:   EG10160
- eggNOG:   COG0737
- GeneTree:   EBGT00050000009523
- HOGENOM:   HBG510269
- OMA:   ENPTTPP
- ProtClustDB:   PRK09420
- BioCyc:   EcoCyc:CPDB-MONOMER
- BioCyc:   MetaCyc:CPDB-MONOMER
- Genevestigator:   P08331
- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843
- Gene3D:   G3DSA:3.90.780.10
- PANTHER:   PTHR11575
- PRINTS:   PR01607
- TIGRFAMs:   TIGR01390

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos; SSF55816 5'-Nucleotdase_C

EC number: =3.1.3.6; =3.1.4.16

Molecular weight: Translated: 70833; Mature: 70833

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00785 5_NUCLEOTIDASE_1; PS00786 5_NUCLEOTIDASE_2

Important sites: BINDING 440-440

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDTATEKFGLVRTASLIN
CEEEHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
DARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYKALNTLDYTVGTLGNHEFN
HHHHHHCCCEEECCCCEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCC
YGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDTEVVDKDGKKQTLKIGYIGVV
CCHHHHHHHHCCCCCCEECCEEEECCCCCCCCCEEEEECCCEECCCCCCEEEEEEEEECC
PPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGADVVVVLAHSGLSADPYKVMAENS
CHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCC
VYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIAKGTLNGVPAVMPGMWGDHLGVVDL
EEEEECCCCCCEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEE
QLSNDSGKWQVTQAKAEARPIYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSA
EECCCCCCEEEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCH
DNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPAS
HHHEEEEEEECCCCCEEEECCHHHHHHHHHHCCCCCHHHCCEECCCCCEECCCCCCCCCC
YVEVEKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQSLIN
EEEEECCEEEEECCCEEEECCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHCC
WDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMINANAERIKNLTFNGKPIDPNAMFLVA
CCCEEEEEEEEECCCCEEEEECCCCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCEEEEE
TNNYRAYGGKFAGTGDSHIAFASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAPIAG
ECCEEECCCEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECC
DKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK
CCEEEEEEECCCCHHHHHHHHHCCCCCCHHCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDTATEKFGLVRTASLIN
CEEEHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
DARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYKALNTLDYTVGTLGNHEFN
HHHHHHCCCEEECCCCEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCC
YGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDTEVVDKDGKKQTLKIGYIGVV
CCHHHHHHHHCCCCCCEECCEEEECCCCCCCCCEEEEECCCEECCCCCCEEEEEEEEECC
PPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGADVVVVLAHSGLSADPYKVMAENS
CHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCC
VYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIAKGTLNGVPAVMPGMWGDHLGVVDL
EEEEECCCCCCEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEE
QLSNDSGKWQVTQAKAEARPIYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSA
EECCCCCCEEEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCH
DNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPAS
HHHEEEEEEECCCCCEEEECCHHHHHHHHHHCCCCCHHHCCEECCCCCEECCCCCCCCCC
YVEVEKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQSLIN
EEEEECCEEEEECCCEEEECCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHCC
WDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMINANAERIKNLTFNGKPIDPNAMFLVA
CCCEEEEEEEEECCCCEEEEECCCCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCEEEEE
TNNYRAYGGKFAGTGDSHIAFASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAPIAG
ECCEEECCCEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECC
DKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK
CCEEEEEEECCCCHHHHHHHHHCCCCCCHHCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3005231; 7610040; 9278503; 2172762; 9298646