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The map label for this gene is ilvB [H]

Identifier: 218691964

GI number: 218691964

Start: 4293054

End: 4294742

Strand: Reverse

Name: ilvB [H]

Synonym: ECED1_4367

Alternate gene names: 218691964

Gene position: 4294742-4293054 (Counterclockwise)

Preceding gene: 218691965

Following gene: 218691963

Centisome position: 82.44

GC content: 56.07

Gene sequence:

>1689_bases
ATGGCAAGTTCGGGCACAACATCGACGCGTAAGCGCTTTACCGGCGCAGAATTTATCGTTCATTTCCTGGAACAGCAGGG
CATTAAGATTGTGACGGGTATTCCGGGCGGTTCTATCCTGCCTGTTTACGATGCCTTAAGCCAAAGCACGCAAATCCGCC
ATATTCTGGCTCGCCATGAACAGGGCGCGGGGTTTATCGCTCAGGGAATGGCGCGCACCGACGGTAAACCGGCGGTCTGT
ATGGCCTGTAGTGGACCGGGTGCGACTAACCTAGTGACCGCCATTGCCGATGCGAGGCTGGACTCCATCCCGCTGATTTG
CATCACTGGTCAGGTTCCCGCCTCGATGATCGGCACCGACGCCTTCCAGGAAGTCGACACCTACGGCATCTCTATCCCCA
TCACCAAATACAACTATCTGGTCAGACATATCGAAGAACTCCCGCAGGTCATGAGCGATGCCTTCCGCATTGCGCAATCA
GGCCGCCCTGGCCCGGTGTGGATAGACATTCCTAAGGATGTGCAAACGGCAGTTTTTGAGATTGAAACACAGCCCGCTAT
GGCAGAAAAAGCCGCCGCACCCGCCTTTAGCGAAGAAAGTATTCGTGACGCGGCGGCGATGATTAACGCCGCCAAACGCC
CGGTGCTTTATCTGGGTGGTGGTGTGATCAACGCGCCTGCGCGAGTGCGTGAACTGGCGGAGAAAGCACAACTGCCTACC
ACCATGACTTTAATGGCGCTGGGTATGTTGCCAAAAGCGCATCCGCTGTCGCTGGGTATGCTGGGGATGCACGGTGTGCG
CAGCACCAACTATATTTTGCAGGAGGCGGATCTGCTGATTGTACTCGGTGCGCGTTTTGATGACCGGGCGATTGGCAAAA
CCGAGCAGTTCTGTCCGAATGCCAAAATTATTCATGTCGATATCGACCGCGCAGAGCTGGGCAAAATCAAGCAACCGCAT
GTGGCAATTCAGGCGGATGTTGATGACGTGTTGGCGCAGTTGATCCCGCTGGTGGAAGCGCAACCGCGTGCAGAGTGGCA
CCAGTTGGTGGCGGATTTGCAGCGTGAATTCCCGTGTCCAATCCCGAAAGCGTGCGATCCATTAAGCCATTACGGCCTGA
TCAACGCCGTTGCCGCCTGTGTCGATGACAATGCGATTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAA
GCCTATCCGCTCAATCGCCCACGCCAGTGGCTGACCTCCGGCGGGCTGGGCACGATGGGTTTTGGCCTGCCTGCGGCGAT
TGGCGCGGCGCTGGCGAACCCGGACCGCAAAGTGTTGTGCTTCTCCGGCGACGGCAGCCTGATGATGAATATTCAGGAGA
TGGCGACCGCCAGTGAAAATCAGCTGGATGTTAAAATCATTTTGATGAACAACGAAGCGCTGGGGCTGGTGCATCAGCAA
CAGAGTCTGTTCTACAAGCAGGGCGTTTTTGCCGCTACCTATCCGGGCAAAATCAACTTTATGCAGATTGCCGCCGGATT
CGGCCTCGAAACCTGTGATTTGAATAACGAAGCCGATCCGCAGGCTGCATTGCAGGAAATCATCAATCGCCCTGGCCCGG
CGCTGATCCATGTGCGCATTGATGCCGAAGAAAAAGTGTACCCGATGGTGCCGCCAGGTGCGGCGAATACTGAAATGGTG
GGGGAATAA

Upstream 100 bases:

>100_bases
GGAACAACACACGATTCCAAAACCCCGCCGGCGCAAACCGGGCGGGGTTTTTCGTTTAAGCTCCTCCCGGAAAGTCGGCC
CAGAAGAAAAGGACTGGAGC

Downstream 100 bases:

>100_bases
GCCATGCAAAACACAACTCATGATAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGTGTAATGACCCACGTTTG
TGGCCTTTTTGCCCGTCGCG

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVC
MACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTDAFQEVDTYGISIPITKYNYLVRHIEELPQVMSDAFRIAQS
GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYLGGGVINAPARVRELAEKAQLPT
TMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPH
VAIQADVDDVLAQLIPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQ
AYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQ
QSLFYKQGVFAATYPGKINFMQIAAGFGLETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMV
GE

Sequences:

>Translated_562_residues
MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVC
MACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTDAFQEVDTYGISIPITKYNYLVRHIEELPQVMSDAFRIAQS
GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYLGGGVINAPARVRELAEKAQLPT
TMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPH
VAIQADVDDVLAQLIPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQ
AYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQ
QSLFYKQGVFAATYPGKINFMQIAAGFGLETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMV
GE
>Mature_561_residues
ASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCM
ACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTDAFQEVDTYGISIPITKYNYLVRHIEELPQVMSDAFRIAQSG
RPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYLGGGVINAPARVRELAEKAQLPTT
MTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHV
AIQADVDDVLAQLIPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQA
YPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQQ
SLFYKQGVFAATYPGKINFMQIAAGFGLETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVG
E

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=569, Percent_Identity=26.0105448154657, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI21361361, Length=557, Percent_Identity=25.673249551167, Blast_Score=168, Evalue=1e-41,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=99.4661921708185, Blast_Score=1151, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=39.2920353982301, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1786717, Length=484, Percent_Identity=36.3636363636364, Blast_Score=289, Evalue=4e-79,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=29.038112522686, Blast_Score=200, Evalue=3e-52,
Organism=Escherichia coli, GI1788716, Length=477, Percent_Identity=26.8343815513627, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=591, Percent_Identity=26.7343485617597, Blast_Score=155, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=560, Percent_Identity=24.6428571428571, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=560, Percent_Identity=24.6428571428571, Blast_Score=148, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=40.7986111111111, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320816, Length=496, Percent_Identity=23.7903225806452, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323073, Length=483, Percent_Identity=21.5320910973085, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922626, Length=477, Percent_Identity=29.3501048218029, Blast_Score=175, Evalue=9e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60450; Mature: 60319

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHCHHHHHHHHHHHCC
QGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTD
CCCCHHHHCHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCH
AFQEVDTYGISIPITKYNYLVRHIEELPQVMSDAFRIAQSGRPGPVWIDIPKDVQTAVFE
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEE
IETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYLGGGVINAPARVRELAEKAQLPT
EECCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCHHHHHHHHHHCCCH
TMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPN
HHHHHHHHCCCCCCCCCEEHHCCCCCCCCCHHEECCCEEEEEECCCCCCCCCCHHHHCCC
AKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQLIPLVEAQPRAEWHQLVADLQREFPCP
CEEEEEECCHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
IPKACDPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQAYPLNRPRQWLTSGGLGTMG
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCHHHHHCCCCCCCC
FGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQ
CCHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHCCCCCCEEEEEEEECCCCEEEEEHH
QSLFYKQGVFAATYPGKINFMQIAAGFGLETCDLNNEADPQAALQEIINRPGPALIHVRI
HHHHHHCCCEEEECCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEE
DAEEKVYPMVPPGAANTEMVGE
CCCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHE
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHCHHHHHHHHHHHCC
QGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTD
CCCCHHHHCHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCH
AFQEVDTYGISIPITKYNYLVRHIEELPQVMSDAFRIAQSGRPGPVWIDIPKDVQTAVFE
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEE
IETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYLGGGVINAPARVRELAEKAQLPT
EECCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCHHHHHHHHHHCCCH
TMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPN
HHHHHHHHCCCCCCCCCEEHHCCCCCCCCCHHEECCCEEEEEECCCCCCCCCCHHHHCCC
AKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQLIPLVEAQPRAEWHQLVADLQREFPCP
CEEEEEECCHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
IPKACDPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQAYPLNRPRQWLTSGGLGTMG
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCHHHHHCCCCCCCC
FGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQ
CCHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHCCCCCCEEEEEEEECCCCEEEEEHH
QSLFYKQGVFAATYPGKINFMQIAAGFGLETCDLNNEADPQAALQEIINRPGPALIHVRI
HHHHHHCCCEEEECCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEE
DAEEKVYPMVPPGAANTEMVGE
CCCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]