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The map label for this gene is nepI [H]

Identifier: 218691954

GI number: 218691954

Start: 4281117

End: 4282472

Strand: Reverse

Name: nepI [H]

Synonym: ECED1_4357

Alternate gene names: 218691954

Gene position: 4282472-4281117 (Counterclockwise)

Preceding gene: 218691955

Following gene: 218691952

Centisome position: 82.2

GC content: 52.21

Gene sequence:

>1356_bases
GTGGATAAGACGTTCACACCGCATCCGGTAATCTTTGCCCTGTTCCGTTTCGATCTTAACTCCAGCTCAAGCAACATCGT
AAAAGTTGCAAAAACGTTAAATGCATCACACATTTCAATGTTAGACTGGTCGGCTTTTCCCCTGAAACATGCCACGGGTA
ACACCATGAGTGAATTTATTGCTGAAAACCGCGGCGCGAATGCCATCACCCGACCGAACTGGTCAGCCGTTTTCTCGGTG
GCGTTTTGCGTCGCCTGTCTGATTATCGTTGAGTTTTTGCCTGTCAGTTTGTTGACGCCAATGGCCCAGGATTTAGGCAT
TTCGGAAGGGGTTGCCGGGCAATCGGTGACCGTGACCGCCTTTGTGGCGATGTTTGCCAGTTTGTTTATTACCCAGACCA
TTCAGGCCACTGACCGCCGCTACGTTGTCATTCTGTTCGCCGTTTTGCTGACGCTTTCCTGTTTGCTGGTTTCCTTTGCC
AACTCATTCAGTCTGCTGTTAATCGGTCGCGCCTGTCTGGGGCTGGCGCTGGGCGGGTTCTGGGCGATGTCGGCGTCGCT
GACCATGCGTCTGGTACCGCCGCGTACGGTGCCGAAGGCGCTGTCGGTGATCTTCGGCGCGGTTTCTATTGCGCTGGTGA
TTGCCGCGCCGTTGGGCAGTTTTTTAGGCGAGCTTATCGGTTGGCGCAATGTCTTTAATGCGGCGGCGGCGATGGGTGTG
CTGTGTATTTTCTGGATTATCAAATCACTGCCTTCGCTGCCAGGTGAACCTTCGCATCAGAAACAAAATACTTTCCGCTT
ATTACAACGTCCGGGAGTGATGGCCGGGATGATCGCCATCTTTATGTCCTTTGCCGGACAGTTTGCTTTTTTCACTTATA
TTCGCCCAGTGTATATGAATCTGGCGGGATTCGGTGTTGATGGCTTAACCCTGGTGCTGCTGAGTTTTGGTATCGCCAGC
TTTGTCGGGACATCGCTTTCGTCGTTTATTCTCAAACGTTCGGTGAAACTGGCCTTAGCGGGCGCACCGTTTGTGCTCGC
CCTGAGCGCGCTGGTACTGACGCTGTGGGGAAGCGATAAAATTGTTGCTACTGGTGTGGCGATTATCTGGGGGCTGACCT
TTGCTTTAATTCCCGTTGGTTGGTCAACGTGGATCACTCGCTCATTGGCCGATCAGGCAGAAAAGGCTGGATCTATTCAG
GTGGCCGTTATTCAGCTTGCTAACACCTGCGGTGCGGCAATCGGCGGTTATGCGTTGGATAACATTGGTCTGACTTCGCC
GCTGATGTTGTCCGGCACATTGATGTTGCTGACTGCATTGTTGGTGACTGCAAAGGTGAAAATGAAGAAATCCTGA

Upstream 100 bases:

>100_bases
GATGCGCTATAACGCGTTCTTTCAGCCTGCCGAGGGCGTTCACTGGCTGCATTAAAACCGCTTCGTCTTATCAGCCTACA
GACCGCGCCTGACCTGGTGG

Downstream 100 bases:

>100_bases
TATCAGGTAAAGAAGCGTTGGTCAGTGGTTGTTGCCGTTCTTACATATCCGGGCATTCTACCTTACCCAACGCTTCCCAA
TAATTGCTCTGATTATTACG

Product: ribonucleoside transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MDKTFTPHPVIFALFRFDLNSSSSNIVKVAKTLNASHISMLDWSAFPLKHATGNTMSEFIAENRGANAITRPNWSAVFSV
AFCVACLIIVEFLPVSLLTPMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLVSFA
NSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIAAPLGSFLGELIGWRNVFNAAAAMGV
LCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGVMAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIAS
FVGTSLSSFILKRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSLADQAEKAGSIQ
VAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVKMKKS

Sequences:

>Translated_451_residues
MDKTFTPHPVIFALFRFDLNSSSSNIVKVAKTLNASHISMLDWSAFPLKHATGNTMSEFIAENRGANAITRPNWSAVFSV
AFCVACLIIVEFLPVSLLTPMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLVSFA
NSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIAAPLGSFLGELIGWRNVFNAAAAMGV
LCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGVMAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIAS
FVGTSLSSFILKRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSLADQAEKAGSIQ
VAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVKMKKS
>Mature_451_residues
MDKTFTPHPVIFALFRFDLNSSSSNIVKVAKTLNASHISMLDWSAFPLKHATGNTMSEFIAENRGANAITRPNWSAVFSV
AFCVACLIIVEFLPVSLLTPMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLVSFA
NSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIAAPLGSFLGELIGWRNVFNAAAAMGV
LCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGVMAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIAS
FVGTSLSSFILKRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSLADQAEKAGSIQ
VAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVKMKKS

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=396, Percent_Identity=98.2323232323232, Blast_Score=768, Evalue=0.0,
Organism=Escherichia coli, GI1787808, Length=243, Percent_Identity=30.8641975308642, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787947, Length=289, Percent_Identity=25.6055363321799, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786595, Length=350, Percent_Identity=25.7142857142857, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47936; Mature: 47936

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKTFTPHPVIFALFRFDLNSSSSNIVKVAKTLNASHISMLDWSAFPLKHATGNTMSEFI
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHH
AENRGANAITRPNWSAVFSVAFCVACLIIVEFLPVSLLTPMAQDLGISEGVAGQSVTVTA
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
FVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLVSFANSFSLLLIGRACLGLALGGF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
WAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIAAPLGSFLGELIGWRNVFNAAAAMGV
HHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGVMAGMIAIFMSFAGQFAFFTYIRPVYMN
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGFGVDGLTLVLLSFGIASFVGTSLSSFILKRSVKLALAGAPFVLALSALVLTLWGSDK
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
IVATGVAIIWGLTFALIPVGWSTWITRSLADQAEKAGSIQVAVIQLANTCGAAIGGYALD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCHHHC
NIGLTSPLMLSGTLMLLTALLVTAKVKMKKS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDKTFTPHPVIFALFRFDLNSSSSNIVKVAKTLNASHISMLDWSAFPLKHATGNTMSEFI
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHH
AENRGANAITRPNWSAVFSVAFCVACLIIVEFLPVSLLTPMAQDLGISEGVAGQSVTVTA
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
FVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLVSFANSFSLLLIGRACLGLALGGF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
WAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIAAPLGSFLGELIGWRNVFNAAAAMGV
HHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGVMAGMIAIFMSFAGQFAFFTYIRPVYMN
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGFGVDGLTLVLLSFGIASFVGTSLSSFILKRSVKLALAGAPFVLALSALVLTLWGSDK
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
IVATGVAIIWGLTFALIPVGWSTWITRSLADQAEKAGSIQVAVIQLANTCGAAIGGYALD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCHHHC
NIGLTSPLMLSGTLMLLTALLVTAKVKMKKS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA