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The map label for this gene is rph [H]

Identifier: 218691927

GI number: 218691927

Start: 4249998

End: 4250738

Strand: Reverse

Name: rph [H]

Synonym: ECED1_4327

Alternate gene names: 218691927

Gene position: 4250738-4249998 (Counterclockwise)

Preceding gene: 218691930

Following gene: 218691926

Centisome position: 81.6

GC content: 55.33

Gene sequence:

>741_bases
TTGTTTCAAGCCGGAGATTTCAATATGCGTCCAGCAGGCCGTAGCAATAATCAGGTGCGTCCCGTTACCCTGACTCGTAA
CTATACAAAACATGCAGAAGGCTCGGTGCTGGTCGAATTTGGCGATACCAAAGTATTGTGTACCGCCTCTATTGAAGAAG
GCGTGCCGCGCTTCCTGAAAGGTCAGGGCCAGGGTTGGATCACCGCAGAGTACGGTATGCTGCCACGTTCTACCCATACC
CGTAACGCGCGTGAAGCGGCGAAAGGTAAGCAGGGTGGACGCACAATGGAAATCCAGCGTCTGATCGCCCGTGCTCTTCG
CGCGGCAGTAGATTTGAAAGCACTGGGTGAGTTCACCATTACGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGC
GTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACT
AATCCAATGAAAGGGATGGTAGCCGCGGTCTCTGTCGGGATTGTGAACGGTGAAGCGGTTTGCGATCTGGAATATGTTGA
AGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGTATCATTGAAGTGCAGGGGACGGCAGAAG
GCGAACCGTTCACCCACGAAGAGTTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAG
AAGGCGGCGCTGGCAAACTGA

Upstream 100 bases:

>100_bases
ACGTTCCTGTTTATAAAAGGAGAGGTGGAAGGATTATAGCCATCGATGCCTTGTCAGGATAGGAATAACCGCCGGAAGTC
CGTATAATGCGCAGCCACAT

Downstream 100 bases:

>100_bases
TTTTTAAGGCGACTGATGAGTCGCCTTTTTTTTGTCTGTAGAAAAGTAAGATGAGGAGCGAAGGCATGAAACCATATCAG
CGCCAGTTTATTGAATTTGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MFQAGDFNMRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWITAEYGMLPRSTHT
RNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKT
NPMKGMVAAVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLLALARGGIESIVATQ
KAALAN

Sequences:

>Translated_246_residues
MFQAGDFNMRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWITAEYGMLPRSTHT
RNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKT
NPMKGMVAAVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLLALARGGIESIVATQ
KAALAN
>Mature_246_residues
MFQAGDFNMRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWITAEYGMLPRSTHT
RNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKT
NPMKGMVAAVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLLALARGGIESIVATQ
KAALAN

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=223, Percent_Identity=99.1031390134529, Blast_Score=452, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI71981632, Length=189, Percent_Identity=28.042328042328, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 26223; Mature: 26223

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQAGDFNMRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLK
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCEEEEEECHHHCCHHHHC
GQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTI
CCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
TLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVAAVSVGIVNGEAV
EEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCEECCCHHHEEEEEEEEEECCCEE
CDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLLALARGGIESIVATQ
EEHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
KAALAN
HHHHCC
>Mature Secondary Structure
MFQAGDFNMRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLK
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCEEEEEECHHHCCHHHHC
GQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTI
CCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
TLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVAAVSVGIVNGEAV
EEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCEECCCHHHEEEEEEEEEECCCEE
CDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLLALARGGIESIVATQ
EEHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
KAALAN
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA