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The map label for this gene is yrbD

Identifier: 218691483

GI number: 218691483

Start: 3780455

End: 3781006

Strand: Reverse

Name: yrbD

Synonym: ECED1_3851

Alternate gene names: 218691483

Gene position: 3781006-3780455 (Counterclockwise)

Preceding gene: 218691484

Following gene: 218691482

Centisome position: 72.58

GC content: 49.46

Gene sequence:

>552_bases
ATGCAAACGAAAAAAAATGAAATTTGGGTGGGTATCTTTTTATTAGCAGCACTGCTTGCGGCGCTGTTTGTTTGCCTGAA
GGCAGCGAACGTGACGTCTATACGTACTGAACCGACCTATACGCTTTATGCGACGTTCGATAACATTGGCGGCCTGAAAG
CCCGCTCTCCGGTCAGCATTGGTGGCGTTGTTGTGGGCCGGGTGGCGGATATTACGCTGGACCCGAAAACCTATCTGCCG
CGCGTAACGCTGGAAATTGAACAACGTTATAACCACATTCCTGATACCAGTTCGCTGAGCATTCGTACTTCCGGCCTGCT
GGGGGAACAATATCTGGCATTAAACGTCGGTTTTGAAGACCCGGAACTGGGAACTGCTATCCTGAAGGATGGCGATACAA
TTCAGGACACCAAGTCTGCGATGGTGCTGGAAGATCTCATTGGTCAGTTCCTTTACGGTAGTAAAGGCGATGACAATAAG
AATAGTGGCGATGCGCCAGCTGCTGCGCCAGGTAATAATGAAACCACTGAACCTGTGGGTACAACGAAATAA

Upstream 100 bases:

>100_bases
GGGATTAGCCGGGCAACCACTCGCACCGTTGTCCACTCGTCTCTGGCTGTTCTGGGGCTGGATTTTGTGCTGACCGCATT
GATGTTTGGGAATTGAGTTC

Downstream 100 bases:

>100_bases
TTTCAGGAGAACCGACGCATGTTTAAACGTTTAATGATGGTCGCTTTGCTGGTGATTGCACCGCTGAGTGCGGCAACCGC
GGCAGACCAGACCAATCCGT

Product: toluene ABC transporter membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYATFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLP
RVTLEIEQRYNHIPDTSSLSIRTSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
NSGDAPAAAPGNNETTEPVGTTK

Sequences:

>Translated_183_residues
MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYATFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLP
RVTLEIEQRYNHIPDTSSLSIRTSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
NSGDAPAAAPGNNETTEPVGTTK
>Mature_183_residues
MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYATFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLP
RVTLEIEQRYNHIPDTSSLSIRTSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
NSGDAPAAAPGNNETTEPVGTTK

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG1463

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, periplasmic component

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaD family

Homologues:

Organism=Escherichia coli, GI1789584, Length=183, Percent_Identity=100, Blast_Score=372, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLAD_ECO57 (P64605)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85983
- PIR:   H91137
- RefSeq:   NP_289767.1
- RefSeq:   NP_312099.1
- ProteinModelPortal:   P64605
- EnsemblBacteria:   EBESCT00000024578
- EnsemblBacteria:   EBESCT00000056193
- GeneID:   916081
- GeneID:   958635
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4556
- KEGG:   ecs:ECs4072
- GeneTree:   EBGT00050000011171
- HOGENOM:   HBG750933
- OMA:   GNDNDYL
- ProtClustDB:   CLSK880618
- BioCyc:   ECOL83334:ECS4072-MONOMER
- GO:   GO:0006810
- InterPro:   IPR003399

Pfam domain/function: PF02470 MCE

EC number: NA

Molecular weight: Translated: 19576; Mature: 19576

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x247878c4)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYATFDNIGGLKARSPVSI
CCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCC
GGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIRTSGLLGEQYLALNVGFED
CCEEEEEEEEEEECCHHHCCEEEEEEEHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCC
PELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNKNSGDAPAAAPGNNETTEPVG
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TTK
CCH
>Mature Secondary Structure
MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYATFDNIGGLKARSPVSI
CCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCC
GGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIRTSGLLGEQYLALNVGFED
CCEEEEEEEEEEECCHHHCCEEEEEEEHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCC
PELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNKNSGDAPAAAPGNNETTEPVG
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TTK
CCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796