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The map label for this gene is dsbA [C]

Identifier: 218691342

GI number: 218691342

Start: 3630897

End: 3631565

Strand: Direct

Name: dsbA [C]

Synonym: ECED1_3705

Alternate gene names: 218691342

Gene position: 3630897-3631565 (Clockwise)

Preceding gene: 218691341

Following gene: 218691343

Centisome position: 69.7

GC content: 45.14

Gene sequence:

>669_bases
ATGTCGAAATTAGGGATCTCATCACTATTTAAAACCATACTGCTTACCGCAGCTCTGGCGGTTTCATTTACCGCTTCTGC
ATTTACCGAAGGCACGGATTACATGGTTCTGGAAAAACCTATTCCGAATGCCGACAAAACGCTGATTAAGGTATTTAGCT
ACGCCTGCCCGTTCTGTTACAAGTACGACAAAGCGGTTACCGGTCCGGTATCGGAAAAAGTGAAAGATATCGTTGCTTTC
ACGCCGTTCCATCTGGAAACCAAAGGTGAATACGGCAAACAAGCAAGCGAAGTCTTTGCGGTCTTGATCAATAAAGATAA
AGCAGCAGGCATTTCCTTATTTGATGCTAACTCTCAGTTTAAGAAAGCCAAGTTTGCCTACTACGCGGCTTATCACGACA
AAAAAGAACGCTGGTCTGACGGTAAAGATCCGGCTGCTTTTATCAAAACCGGTCTGGATGCCGCAGGTATGAGTCAGGCG
GATTTTGAAGCGGCACTGAAAGAACCAGCAGTTCAAGAAACACTGGAAAAATGGAAAGCCTCTTATGATGTCGCCAAAAT
CCAGGGGGTTCCAGCCTATGTCGTTAATGGTAAGTACTTGATCTACACCAAGAGCATCAAATCCATCGACGCGATGGCAG
ACCTTATCCGTGAGTTAGCAAGCAAATAA

Upstream 100 bases:

>100_bases
TGGAAATCGACGTGCTGTCAGATAAACCCAATCAGACTCACTACCGTGCGCTGTTAGTCCGTCCACAACAGATGTTCAAA
TAATTAAGAAGGGAATAAAA

Downstream 100 bases:

>100_bases
CAGGGAAACAGGCTATGGGTATTAAGGGAATGTGGAAAGATCTTCGCACATCACCAGTTGACACACTGGTCAGATGGCAG
GAGCAACGCTTGTTGTGGTT

Product: Thiol:disulfide interchange protein dsbA

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAF
TPFHLETKGEYGKQASEVFAVLINKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRELASK

Sequences:

>Translated_222_residues
MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAF
TPFHLETKGEYGKQASEVFAVLINKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRELASK
>Mature_221_residues
SKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFT
PFHLETKGEYGKQASEVFAVLINKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQAD
FEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRELASK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain

Homologues:

Organism=Escherichia coli, GI1790291, Length=227, Percent_Identity=28.6343612334802, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DSBL_ECOL6 (P0A4L7)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_755657.1
- PDB:   3C7M
- PDBsum:   3C7M
- ProteinModelPortal:   P0A4L7
- SMR:   P0A4L7
- EnsemblBacteria:   EBESCT00000044922
- GeneID:   1039926
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c3786
- GeneTree:   EBGT00050000010220
- HOGENOM:   HBG680642
- OMA:   ANEIFAF
- ProtClustDB:   CLSK894418
- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- PIRSF:   PIRSF001488

Pfam domain/function: PF01323 DSBA; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 24381; Mature: 24250

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS00194 THIOREDOXIN_1; PS51352 THIOREDOXIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHH
KYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVLINKDKAAGISLFDANSQF
HCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCHH
KKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQADFEAALKEPAVQETLEKWKA
HHHHHHHHHHHHCHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
SYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRELASK
HCCHHHHCCCCEEEECCEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCY
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHH
KYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVLINKDKAAGISLFDANSQF
HCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCHH
KKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQADFEAALKEPAVQETLEKWKA
HHHHHHHHHHHHCHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
SYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRELASK
HCCHHHHCCCCEEEECCEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157