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The map label for this gene is hybA [H]

Identifier: 218691287

GI number: 218691287

Start: 3575493

End: 3576479

Strand: Reverse

Name: hybA [H]

Synonym: ECED1_3646

Alternate gene names: 218691287

Gene position: 3576479-3575493 (Counterclockwise)

Preceding gene: 218691288

Following gene: 218691286

Centisome position: 68.65

GC content: 54.0

Gene sequence:

>987_bases
GTGAACAGACGTAATTTTATTAAAGCAGCCTCCTGCGGGGCATTGCTGACGGGCGCGCTGCCGTCTGTCAGTCATGCGGC
TGCTGAAAACCGCCCGCCAATTCCGGGATCGCTGGGGATGTTGTACGACTCGACCTTGTGCGTAGGCTGTCAGGCTTGCG
TCACCAAGTGTCAGGATATCAACTTCCCTGAACGTAACCCGCAAGGGGAACAGACCTGGTCGAACAACGACAAACTGTCG
CCGTATACCAATAACATCATTCAGGTGTGGACCAGCAGCACAGGGGTCAACAAAGACCAGGAGGAGAATGGCTACGCGTA
CATTAAGAAACAGTGTATGCACTGCGTCGATCCGAACTGTGTCTCTGTGTGTCCGGTCTCTGCACTGAAAAAAGATCCGA
AAACCGGTATTGTCCATTACGACAAAGATGTGTGCACCGGTTGCCGTTACTGCATGGTCGCCTGCCCATACAACGTGCCG
AAGTACGACTACAACAACCCGTTTGGTGCGCTGCATAAGTGCGAGTTGTGCAACCAGAAAGGTGTGGAACGTCTCGATAA
AGGCGGTCTGCCTGGCTGTGTAGAAGTGTGCCCGGCGGGCGCGGTGATTTTTGGTACGCGTGAAGAGCTGATGGCGGAGG
CGAAAAAACGTCTGGCGCTGAAGCCGGGCAGCGAATACCACTATCCGCGTCAGACGCTGAAATCTGGCGACACTTACCTG
CATACGGTGCCGAAATATTATCCGCATCTGTACGGCGAGAAAGAGGGCGGCGGTACTCAGGTTCTGGTACTGACGGGTGT
GCCTTATGAAAATCTCGACCTGCCGAAACTGGACGATCTTTCTACTGGTGCGCGTTCCGAACATGTCCAACACACCCTGT
ATAAAGGCATGATGCTACCACTGGCTGTGCTGGCGGGCTTAACCGTGCTGGTTCGTCGCAACACTAAAAACGACCATCAC
GACGGAGGAGACGATCATGAGTCATGA

Upstream 100 bases:

>100_bases
GCGGTGTGGTTGGGCTGGTTGCCGGTGTCAGCGTGATGGCGGTGCGTGAACTGGGTCGTCAGCAAAAGAAAGATAACGCT
GACTCACGGGGAGAATAACC

Downstream 100 bases:

>100_bases
TCCACAACCGCTGGGCGGCAAAATCATCAGTAAACCGGTCATGATTTTTGGACCGTTAATCGTCATCTGTATGCTCCTGA
TTGTGAAGCGTCTGGTGTTC

Product: hydrogenase 2 protein HybA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLS
PYTNNIIQVWTSSTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSEHVQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHH
DGGDDHES

Sequences:

>Translated_328_residues
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLS
PYTNNIIQVWTSSTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSEHVQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHH
DGGDDHES
>Mature_328_residues
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLS
PYTNNIIQVWTSSTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSEHVQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHH
DGGDDHES

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=328, Percent_Identity=99.0853658536585, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI2367345, Length=255, Percent_Identity=30.1960784313725, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787749, Length=283, Percent_Identity=27.5618374558304, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1790326, Length=184, Percent_Identity=33.1521739130435, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787872, Length=198, Percent_Identity=34.3434343434343, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787122, Length=198, Percent_Identity=33.8383838383838, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI226510944, Length=209, Percent_Identity=31.1004784688995, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1787478, Length=133, Percent_Identity=33.8345864661654, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787740, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082180, Length=192, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789067, Length=197, Percent_Identity=28.9340101522843, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36042; Mature: 36042

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDI
CCCCHHHHHHHCCHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSSTGVNKDQEENGYAYIKKQCMHCVDPNC
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVPKYDYNNPFGALHKCELCNQK
HHCCCHHHHHCCCCCCCEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
GVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HHHHHHCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEE
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSEHVQHTLYKGMMLP
EHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAVLAGLTVLVRRNTKNDHHDGGDDHES
HHHHHHHHEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDI
CCCCHHHHHHHCCHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSSTGVNKDQEENGYAYIKKQCMHCVDPNC
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVPKYDYNNPFGALHKCELCNQK
HHCCCHHHHHCCCCCCCEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
GVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HHHHHHCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEE
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSEHVQHTLYKGMMLP
EHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAVLAGLTVLVRRNTKNDHHDGGDDHES
HHHHHHHHEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]