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The map label for this gene is glcD [H]

Identifier: 218691264

GI number: 218691264

Start: 3550649

End: 3552148

Strand: Reverse

Name: glcD [H]

Synonym: ECED1_3622

Alternate gene names: 218691264

Gene position: 3552148-3550649 (Counterclockwise)

Preceding gene: 218691266

Following gene: 218691263

Centisome position: 68.19

GC content: 57.0

Gene sequence:

>1500_bases
ATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGAAGTCGACCGCACATCGGTACTGATGGCACTGCGTGA
GCATGTCCCTGGACTGGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCA
CGCGTCCATTACTGGTTGTTCTGCCTAAGCAGATGGAACAGGTGACGGCGATTCTGGCTGTCTGCCACCGCCTGCGTGTG
CCGGTGGTGACCCGTGGCGCAGGCACCGGGCTTTCTGGTGGCGCGCTGCCGCTGGAAAAAGGTGTGTTGTTGGTGATGGC
GCGCTTTAAAGAGATCCTCGACATTAACCCCATTGGTCGCCGCGCGCGCGTGCAGCCGGGCGTGCGTAACCTGGCGATCT
CCCAGGCCGTTGCGCCGCATAATCTCTATTACGCGCCGGATCCGTCCTCACAAATCGCCTGTTCGATTGGCGGCAATGTG
GCGGAAAATGCCGGTGGCGTTCACTGCCTGAAATATGGTCTAACCGTACATAACCTGCTGAAAATTGAAGTGCAAACGCT
GGATGGCGAGGCGCTGACGCTGGGATCGGACGCGCTGGATTCGCCTGGTTTTGACCTGCTGGCGCTGTTTACCGGATCGG
AAGGTATGCTCGGCGTGACCACCGAAGTAACGGTGAAGCTGTTGCCGAAGCCGCCCGTGGCGCGGGTTCTGTTAGCCAGC
TTTGACTCGGTAGAAAAAGCCGGACTTGCGGTTGGTGGCATCATCGCCAATGGCATTATCCCCGGCGGGCTGGAGATGAT
GGATAACCTGTCGATTCGCGCGGCGGAAGATTTTATTCATGCCGGTTATCCCGTCGATGCCGAAGCGATTTTGTTATGCG
AACTGGACGGCGTGGAGTCTGACGTACAGGAAGACTGCGAGCGGGTTAACGACATCTTGTTGAACGCGGGTGCGACTGGC
GTCCGTCTGGCACAGGACGAAGCAGAACGCGTACGTTTCTGGGCCGGTCGCAAAAATGCGTTCCCGGCGGTAGGACGTAT
CTCCCCGGATTACTACTGCATGGATGGCACCATCCCGCGCCGCGCCCTGCCTGGCGTACTGGAAGGCATTGCCCGTTTAT
CGCAGCAATATGATTTACGCGTTGCCAACGTCTTTCATGCCGGAGACGGCAACATGCACCCGTTAATCCTTTTCGATGCC
AACGAACCCGGTGAATTTGCCCGCGCGGAAGAACTGGGCGGGAAGATCCTCGAACTCTGCGTTGAAGTTGGCGGCAGCAT
CAGTGGGGAACATGGCATCGGGCGCGAAAAAATCAATCAAATGTGCGCCCAGTTCAACAGCGATGAAATCACGACCTTCC
ATGAGGTCAAGGCGGCGTTTGATCCCGATGGTTTGCTGAACCCTGGGAAAAACATTCCCACGCTACACCGCTGTGCTGAA
TTTGGTGCCATGCATGTGCATCACGGTCATTTACCTTTCCCTGAACTGGAGCGTTTCTGA

Upstream 100 bases:

>100_bases
TAAAGAGATAGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGAAAAAATCAGCTGCCACACAACA
CAACAAAGCGAACCCTACTC

Downstream 100 bases:

>100_bases
TGCTACGCGAGTGTGATTACAGCCAGGCGCTGCTGGAGCAGGTGAATCAGGCGATTAGCGATAAAACGCCGCTGGTGATT
CAGGGCAGCAATAGCAAAGC

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSILYEERLDGALPEVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRV
PVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPIGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLAS
FDSVEKAGLAVGGIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLNAGATG
VRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDA
NEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHEVKAAFDPDGLLNPGKNIPTLHRCAE
FGAMHVHHGHLPFPELERF

Sequences:

>Translated_499_residues
MSILYEERLDGALPEVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRV
PVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPIGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLAS
FDSVEKAGLAVGGIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLNAGATG
VRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDA
NEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHEVKAAFDPDGLLNPGKNIPTLHRCAE
FGAMHVHHGHLPFPELERF
>Mature_498_residues
SILYEERLDGALPEVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVP
VVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPIGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVA
ENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASF
DSVEKAGLAVGGIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLNAGATGV
RLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDAN
EPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHEVKAAFDPDGLLNPGKNIPTLHRCAEF
GAMHVHHGHLPFPELERF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=425, Percent_Identity=30.3529411764706, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI37595754, Length=448, Percent_Identity=29.9107142857143, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI119964728, Length=462, Percent_Identity=28.7878787878788, Blast_Score=159, Evalue=7e-39,
Organism=Escherichia coli, GI1789351, Length=499, Percent_Identity=98.7975951903808, Blast_Score=998, Evalue=0.0,
Organism=Escherichia coli, GI1787977, Length=503, Percent_Identity=24.0556660039761, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71992373, Length=431, Percent_Identity=27.8422273781903, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI71992381, Length=427, Percent_Identity=26.2295081967213, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71992392, Length=359, Percent_Identity=27.0194986072423, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17534361, Length=474, Percent_Identity=28.6919831223629, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320023, Length=453, Percent_Identity=28.6975717439294, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6320764, Length=491, Percent_Identity=26.4765784114053, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6320027, Length=436, Percent_Identity=25.9174311926606, Blast_Score=156, Evalue=9e-39,
Organism=Drosophila melanogaster, GI18921117, Length=444, Percent_Identity=28.1531531531532, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24639277, Length=444, Percent_Identity=28.1531531531532, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24639275, Length=444, Percent_Identity=28.1531531531532, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24653753, Length=222, Percent_Identity=25.2252252252252, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 53768; Mature: 53637

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILYEERLDGALPEVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVV
CCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCHHCCCCEEEE
LPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPIGR
CCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCC
RARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL
CCCCCCCHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCEEEEE
KIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLAS
EEEEEEECCCEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHH
FDSVEKAGLAVGGIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVES
HHHHHHHCCCHHHHHHCCCCCCCHHHHHCCCEEEHHHHHHCCCCCCCCEEEEEECCCCCH
DVQEDCERVNDILLNAGATGVRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPR
HHHHHHHHHHHHEECCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCH
RALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEPGEFARAEELGGKILELC
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
VEVGGSISGEHGIGREKINQMCAQFNSDEITTFHEVKAAFDPDGLLNPGKNIPTLHRCAE
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCCCCCCCCCHHHHHHH
FGAMHVHHGHLPFPELERF
HCCEEEECCCCCCHHHHCC
>Mature Secondary Structure 
SILYEERLDGALPEVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVV
CCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCHHCCCCEEEE
LPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPIGR
CCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCC
RARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL
CCCCCCCHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCEEEEE
KIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLAS
EEEEEEECCCEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHH
FDSVEKAGLAVGGIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVES
HHHHHHHCCCHHHHHHCCCCCCCHHHHHCCCEEEHHHHHHCCCCCCCCEEEEEECCCCCH
DVQEDCERVNDILLNAGATGVRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPR
HHHHHHHHHHHHEECCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCH
RALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEPGEFARAEELGGKILELC
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
VEVGGSISGEHGIGREKINQMCAQFNSDEITTFHEVKAAFDPDGLLNPGKNIPTLHRCAE
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCCCCCCCCCHHHHHHH
FGAMHVHHGHLPFPELERF
HCCEEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]