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The map label for this gene is epsK [H]

Identifier: 218691248

GI number: 218691248

Start: 3528464

End: 3529441

Strand: Reverse

Name: epsK [H]

Synonym: ECED1_3605

Alternate gene names: 218691248

Gene position: 3529441-3528464 (Counterclockwise)

Preceding gene: 218691249

Following gene: 218691247

Centisome position: 67.75

GC content: 55.42

Gene sequence:

>978_bases
ATGATCACCTCACCACCAAAACGCGGAATGGCACTGGTCGTGGTGCTGGTATTGCTGGCGGTCATAATGCTGGTAACCAT
CACGCTTTCCAGTCGGATGCAGCAACAACTTGGGCGAACGCGCAGCCAGCAGGAGTACCAGCAGGCGCTGTGGTACAGCG
CCAGTGCAGAAAGCCTGGCGCTGAGCGCGCTCAGTCTGAGCCTGAAAAATGAAAAGCGTGTGCATCTGGCACAGCCGTGG
GCTTCTGGCCCGCGTTTTTTCCCACTGCCGCAGGGGCAAATTGCCGTCACTCTGCGTGACGCACAGGCCTGCTTTAACCT
GAATGCCCTCGCTCAGCCGACGACGGCGTCGCGTCCGCTCGCGGTACAACAACTGATTGCCCTGATCTCGCGCCTCGATG
TGCCTGCTTATCGGGCCGAACTGATAGCCGAAAGCCTGTGGGAGTTTATTGACGAAGACCGCAGCGTGCAGACGCGTCTG
GGTCGTGAAGACAGCGAGTATCTCGCCCGCTCGGTGCCGTTCTACGCCGCTAATCAACCGCTGGCTGATATCAGCGAGAT
GCGCGTGGTGCAGGGAATGGACGCCGGGCTTTATCAAAAACTGAAACCGTTGGTCTGTGCGCTGCCGATGGCCCGCCAGC
AAATCAACATCAATACATTAGATGTCACGCAAAGTGTGATTCTTGAGGCGCTGTTTGACCCGTGGTTAAGCCCTGTTCAG
GCGCGGGCATTATTACAACAACGTCCGGCGAAGGGCTGGGAAGATGTCGATCAGTTTCTTGCTCAGCCGCTACTTGCAGA
CGTCGATGAGCGTACTAAAAAACAGCTAAAAACCATCCTGAGCGTGGACAGCAATTACTTCTGGCTGCGTTCAGATATCA
CCGTGAATGAGATTGAACTGACGATGAATTCGTTAATTGTCCGCATGGGCCCACAACACTTTTCTGTTCTCTGGCATCAG
ACAGGAGAAAGTGAGTGA

Upstream 100 bases:

>100_bases
CATCAGTGCAGGCGATTCCTGTGGCAGTACGCATAACCCTGCATTCGCCACAATGGGGCGAAATTGAACGCATCTGGTTG
TTACGCGGGCCGCAATTATC

Downstream 100 bases:

>100_bases
GTTCCATGCTTGAGATTTTTTTCCCGCTTTGCGCCGCTGATCCCATCCGTTGGCAGCGCCATACACCCGACGTGGAGCAC
GGTATCTGGCCTGACGTTGC

Product: GspK-like type II secretion protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsK [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MITSPPKRGMALVVVLVLLAVIMLVTITLSSRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPW
ASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININTLDVTQSVILEALFDPWLSPVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTILSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQ
TGESE

Sequences:

>Translated_325_residues
MITSPPKRGMALVVVLVLLAVIMLVTITLSSRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPW
ASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININTLDVTQSVILEALFDPWLSPVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTILSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQ
TGESE
>Mature_325_residues
MITSPPKRGMALVVVLVLLAVIMLVTITLSSRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPW
ASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININTLDVTQSVILEALFDPWLSPVQ
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTILSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQ
TGESE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=285, Percent_Identity=34.7368421052632, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 36525; Mature: 36525

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSPPKRGMALVVVLVLLAVIMLVTITLSSRMQQQLGRTRSQQEYQQALWYSASAESLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHH
LSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPL
HHHHHHHHCCCCCEEEECCCCCCCEECCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCH
AVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCCCCCC
LADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININTLDVTQSVILEALFDPWLSPVQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTILSVDSNYFWLRSDITVNEIEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
TMNSLIVRMGPQHFSVLWHQTGESE
HHHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MITSPPKRGMALVVVLVLLAVIMLVTITLSSRMQQQLGRTRSQQEYQQALWYSASAESLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHH
LSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPL
HHHHHHHHCCCCCEEEECCCCCCCEECCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCH
AVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCCCCCCC
LADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININTLDVTQSVILEALFDPWLSPVQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTILSVDSNYFWLRSDITVNEIEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
TMNSLIVRMGPQHFSVLWHQTGESE
HHHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]