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The map label for this gene is bfpB [H]

Identifier: 218691155

GI number: 218691155

Start: 3433848

End: 3435449

Strand: Direct

Name: bfpB [H]

Synonym: ECED1_3504

Alternate gene names: 218691155

Gene position: 3433848-3435449 (Clockwise)

Preceding gene: 218691154

Following gene: 218691156

Centisome position: 65.91

GC content: 53.0

Gene sequence:

>1602_bases
ATGAAAAAATTTTTCTGCGCTGTCACCGCCTTATCCGTTGTTTTATCAGGCTGCAGCAGTTTTGAACGAATCAGTGCCAC
AGAAAAAAAAGCATCCGATGAGATAACACGGGCTGAACGTATAGCCGGTGAACTTCGCAAACAACGCGCCGTTGTGCGCG
AAACAAACCAGCAGTGGATCAATACCACGCCCCTTCCGGAAAAAACCGTTACCCGGGCAATACCGGATTGCCCGGTCGTC
ATAAACACCCGGGAAGCCATCACACTTCACCAGATTGCACAGCGGATTTCTGAAACCTGTCATATTCCGGTTCAAATCAT
GTCTGATGTCTGGACACTGCTGGGTACCGGAGAAAATCGCACACAGAGGATCAGCGGCGACATTCCCCCGCCTGATACCA
GTGGCATGCAGCCACTGAGCACCGTGGGGCTGTCATCAGCCGCCCCCTCCACGGCCTCACTGCCGGAACTCAATGTTCAG
GGGCTGCCCGCTTTGCTTGACACCGTATCTTCACGCTTTGGGATCACCTGGCGCTATGTGAAGGGAAAAATTGAATTCCA
CTGGCTTGACACCCGTACATTCCCGGTCACCTACATGGACAGCCAGGTCGCCTACAATGCCAGAGTCGTCAGTGGCACCA
TGTCATCCACGGGCAGTTCCGGTGGTTCATCCGGCAGTTCCCTGAGTGGGGATGCCAGCAATACGCAGACAACCGCCGTT
GATATGAAATCCACACTCTATGAAGACACAAAAAATGCCGTGAATTCCATGCTGACGCCCGGTATCGGAAGGATGGTGTT
GTCCACCGGCTTTCTGACCGTAACCGACACCCCACAGGTTCTGGATACTGTTAAGAATTTTATGGAAGAACGTAACCGGG
AAATGCGCCGTCAGGTTGTGCTGAATATTGAAATTCTGAGCATCAAAAAAACCAGCAAAGAGCAGGCCGGAATCGACTGG
AACGCCGTTTTCAGTGACGGACATCTCGGTATCGACCTGACCAGTGCATTTACTAATGCCGCCGATGAAGTCGTGACCAG
TGGGGTATCGATTATTGACGGTAAGCTCACCGGTTCAAAAGCCTTTCTGAAAGCGCTTTCATCACAGGGTAAAGTCAGTG
TGGTCACCCAAAACTCGGCTGTCACCAAAAACCTGACGCCGGTTCCTATGCAGGTCGCCAGCCAGCAGGGGTACATAGAA
AGTGTCACCACGGATACGACGGCCAATGTGGGCTCCTCCACCTCACTGAATGCGGCAACAATCACCACCGGCTTCAACAT
GACACTGTTACCGTTTATCCAGCCCGGCTCACACCGGTTGCAGTTACTGTTCAGCATGAGCCTGTCCGACAAACCTGTTT
TTGAAGTGTTTGAAAGCGGTGGCAGTCGGGCCCAGACACCGAGTGTTGAGCTGAAAACCATCAACCAGACCATCGACCTG
AAATCAGATCAGACGGTGATCCTGAGCGGATTCCAGCAGTTCAGCCGGAAATCGCTCAGACAGGGCGTGGGCACCCCGTC
ATTCTTCGGACTCGGTGGTGGCGTGGACAGTGCGGATGACGACACCATTCTGGTTGTGCTGATCACACCGCACGTAATCT
GA

Upstream 100 bases:

>100_bases
TTCATTAACACATGAAATACCCGTCGCTCTGTCTCACTGAATTGTTATTTCGTCCCTCCACAGACAGAGAATAAATATTC
AACAAGCAGGTATACAGAAT

Downstream 100 bases:

>100_bases
CGGAGGTTCCCTTGAACGGGTCAATGCAGAAAAAAATTCGTCCGGTTTCACTGATATCGGGTAAACAGGCATTCCTTGCG
GGCCTTGACTGGAAGAACAT

Product: putative type IV pilus outer membrane secretin lipoprotein

Products: NA

Alternate protein names: Bundle-forming pilus B [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKKFFCAVTALSVVLSGCSSFERISATEKKASDEITRAERIAGELRKQRAVVRETNQQWINTTPLPEKTVTRAIPDCPVV
INTREAITLHQIAQRISETCHIPVQIMSDVWTLLGTGENRTQRISGDIPPPDTSGMQPLSTVGLSSAAPSTASLPELNVQ
GLPALLDTVSSRFGITWRYVKGKIEFHWLDTRTFPVTYMDSQVAYNARVVSGTMSSTGSSGGSSGSSLSGDASNTQTTAV
DMKSTLYEDTKNAVNSMLTPGIGRMVLSTGFLTVTDTPQVLDTVKNFMEERNREMRRQVVLNIEILSIKKTSKEQAGIDW
NAVFSDGHLGIDLTSAFTNAADEVVTSGVSIIDGKLTGSKAFLKALSSQGKVSVVTQNSAVTKNLTPVPMQVASQQGYIE
SVTTDTTANVGSSTSLNAATITTGFNMTLLPFIQPGSHRLQLLFSMSLSDKPVFEVFESGGSRAQTPSVELKTINQTIDL
KSDQTVILSGFQQFSRKSLRQGVGTPSFFGLGGGVDSADDDTILVVLITPHVI

Sequences:

>Translated_533_residues
MKKFFCAVTALSVVLSGCSSFERISATEKKASDEITRAERIAGELRKQRAVVRETNQQWINTTPLPEKTVTRAIPDCPVV
INTREAITLHQIAQRISETCHIPVQIMSDVWTLLGTGENRTQRISGDIPPPDTSGMQPLSTVGLSSAAPSTASLPELNVQ
GLPALLDTVSSRFGITWRYVKGKIEFHWLDTRTFPVTYMDSQVAYNARVVSGTMSSTGSSGGSSGSSLSGDASNTQTTAV
DMKSTLYEDTKNAVNSMLTPGIGRMVLSTGFLTVTDTPQVLDTVKNFMEERNREMRRQVVLNIEILSIKKTSKEQAGIDW
NAVFSDGHLGIDLTSAFTNAADEVVTSGVSIIDGKLTGSKAFLKALSSQGKVSVVTQNSAVTKNLTPVPMQVASQQGYIE
SVTTDTTANVGSSTSLNAATITTGFNMTLLPFIQPGSHRLQLLFSMSLSDKPVFEVFESGGSRAQTPSVELKTINQTIDL
KSDQTVILSGFQQFSRKSLRQGVGTPSFFGLGGGVDSADDDTILVVLITPHVI
>Mature_533_residues
MKKFFCAVTALSVVLSGCSSFERISATEKKASDEITRAERIAGELRKQRAVVRETNQQWINTTPLPEKTVTRAIPDCPVV
INTREAITLHQIAQRISETCHIPVQIMSDVWTLLGTGENRTQRISGDIPPPDTSGMQPLSTVGLSSAAPSTASLPELNVQ
GLPALLDTVSSRFGITWRYVKGKIEFHWLDTRTFPVTYMDSQVAYNARVVSGTMSSTGSSGGSSGSSLSGDASNTQTTAV
DMKSTLYEDTKNAVNSMLTPGIGRMVLSTGFLTVTDTPQVLDTVKNFMEERNREMRRQVVLNIEILSIKKTSKEQAGIDW
NAVFSDGHLGIDLTSAFTNAADEVVTSGVSIIDGKLTGSKAFLKALSSQGKVSVVTQNSAVTKNLTPVPMQVASQQGYIE
SVTTDTTANVGSSTSLNAATITTGFNMTLLPFIQPGSHRLQLLFSMSLSDKPVFEVFESGGSRAQTPSVELKTINQTIDL
KSDQTVILSGFQQFSRKSLRQGVGTPSFFGLGGGVDSADDDTILVVLITPHVI

Specific function: Is absolutely required for pilus biogenesis, and for EPEC localized adherence and autoaggregation. Acts at a step in the BFP biogenic pathway after production and processing of the structural pilus subunit BfpA [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011514
- InterPro:   IPR004846 [H]

Pfam domain/function: PF00263 Secretin; PF07655 Secretin_N_2 [H]

EC number: NA

Molecular weight: Translated: 57278; Mature: 57278

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFFCAVTALSVVLSGCSSFERISATEKKASDEITRAERIAGELRKQRAVVRETNQQWI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
NTTPLPEKTVTRAIPDCPVVINTREAITLHQIAQRISETCHIPVQIMSDVWTLLGTGENR
CCCCCCHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
TQRISGDIPPPDTSGMQPLSTVGLSSAAPSTASLPELNVQGLPALLDTVSSRFGITWRYV
CEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEE
KGKIEFHWLDTRTFPVTYMDSQVAYNARVVSGTMSSTGSSGGSSGSSLSGDASNTQTTAV
EEEEEEEEECCCEEEEEEECCCCEECEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEE
DMKSTLYEDTKNAVNSMLTPGIGRMVLSTGFLTVTDTPQVLDTVKNFMEERNREMRRQVV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHEE
LNIEILSIKKTSKEQAGIDWNAVFSDGHLGIDLTSAFTNAADEVVTSGVSIIDGKLTGSK
EEEEEEEEECCCHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHCCCEEEECCCCCHH
AFLKALSSQGKVSVVTQNSAVTKNLTPVPMQVASQQGYIESVTTDTTANVGSSTSLNAAT
HHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCEEE
ITTGFNMTLLPFIQPGSHRLQLLFSMSLSDKPVFEVFESGGSRAQTPSVELKTINQTIDL
EECCCCEEEEEEECCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCCCEEEEEECEEEEC
KSDQTVILSGFQQFSRKSLRQGVGTPSFFGLGGGVDSADDDTILVVLITPHVI
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MKKFFCAVTALSVVLSGCSSFERISATEKKASDEITRAERIAGELRKQRAVVRETNQQWI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
NTTPLPEKTVTRAIPDCPVVINTREAITLHQIAQRISETCHIPVQIMSDVWTLLGTGENR
CCCCCCHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
TQRISGDIPPPDTSGMQPLSTVGLSSAAPSTASLPELNVQGLPALLDTVSSRFGITWRYV
CEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEE
KGKIEFHWLDTRTFPVTYMDSQVAYNARVVSGTMSSTGSSGGSSGSSLSGDASNTQTTAV
EEEEEEEEECCCEEEEEEECCCCEECEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEE
DMKSTLYEDTKNAVNSMLTPGIGRMVLSTGFLTVTDTPQVLDTVKNFMEERNREMRRQVV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHEE
LNIEILSIKKTSKEQAGIDWNAVFSDGHLGIDLTSAFTNAADEVVTSGVSIIDGKLTGSK
EEEEEEEEECCCHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHCCCEEEECCCCCHH
AFLKALSSQGKVSVVTQNSAVTKNLTPVPMQVASQQGYIESVTTDTTANVGSSTSLNAAT
HHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCEEE
ITTGFNMTLLPFIQPGSHRLQLLFSMSLSDKPVFEVFESGGSRAQTPSVELKTINQTIDL
EECCCCEEEEEEECCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCCCEEEEEECEEEEC
KSDQTVILSGFQQFSRKSLRQGVGTPSFFGLGGGVDSADDDTILVVLITPHVI
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8626330; 10496929; 8932312 [H]