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The map label for this gene is mexB [H]

Identifier: 218691131

GI number: 218691131

Start: 3412806

End: 3413870

Strand: Reverse

Name: mexB [H]

Synonym: ECED1_3476

Alternate gene names: 218691131

Gene position: 3413870-3412806 (Counterclockwise)

Preceding gene: 218691133

Following gene: 218691130

Centisome position: 65.53

GC content: 46.57

Gene sequence:

>1065_bases
ATGATCCAGGCCCGAAATCAGCTTCTTGCTGAAGCGGCGAAAAGTCCAGCGTTAAACATGGTTCGTCCTAACGGCATGAA
CGATGAGCCTCAGTTCCAGATTCTGATTGATGACGAGAAAGTTCAGGCGTTTAAACTGAGCATGAGCGACGTCGACAACA
TAATGTCAGCAGCCTGGGGTTCGATGTATGTAAATGATTTTAACGATCGTGGACGGGTGAAAAAAGTCTATATTCAGGGC
GAACCCGGCTCACGTATATCACCGCAGGATTTTGATAAATGGTATGTCCGTAACAGTGATGGCGACATGGTTTCCTTTGC
CTCTTTTGCAACAGGCAAATGGATTTATGGCTCACCAAAACTGGAGCAATATAATGGTATCTCTGCTGTCGAAATCCTGG
GGGAACCGGCACCAGGATACAGTTCCGGGGATGCAATGAAAGCAATTGAGGATATTGCGGCAAGACTTCCCGAAGGTTTC
CATATTTCCTGGACAGGTTTGTCTTTTGAGGAACGACTTTCAGGTTCTCAGGCTCCTGCACTATATGCACTTTCACTGTT
GATCGTCTTTCTGTGTCTGGCTGCACTGTATGAAAGCTGGAGCATTCCTTTCTCGGTCATGCTGGTTGTTCCGCTTGGTG
TCCTGGGGGCTGTCTGTGCTACTTTGTTGCGCGGCCTTGGTAACGATGTCTTTTTCCAGGTCGGTTTATTAACAACAATA
GGGCTGTCTGCGAAAAATGCCATACTTATCGTGGAGTTTGCTCGTGAATTGCATGAGAAAGAAGGACTAAGCATTAAAGA
GGCTGCTGTTGAAGCGGCGAGAGTGAGGTTAAGACCGATAATTATGACTTCGCTGGCATTTGTAATGGGGGTTATCCCAC
TGGCTGTCTCGACTGGAGCAAGTTCCGGAAGTAAACATGCCATCGGTACTGGTGTGGTTGGAGGGATGATCACTGCTACG
ATACTGGCAATCTTTTATATTCCTCTCTTCTATATGTTAATTGCCGGGTTCTTCAGCCGCAGAAATAAGAAATCATCGGA
TAAGACTGAAGCTGAGCAATCATAA

Upstream 100 bases:

>100_bases
CTAGTCTAGTTTTAAAGCGCCCCCTGCCGTATCAGAACTGGGGAATGCTACAGGGTTTGACCTGTTCCTCGAGGATCGTA
GCGGAGCTAGCCATGAAAAA

Downstream 100 bases:

>100_bases
GCATGAGTAAATATTCAGGTGCAGTAAACAACATACTGCGCCTGAGTAAATGGCTGTTGGGTTGGTACTAAATGCCCCAG
AGATATTTCCTGAAATAAGA

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter MexB [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MIQARNQLLAEAAKSPALNMVRPNGMNDEPQFQILIDDEKVQAFKLSMSDVDNIMSAAWGSMYVNDFNDRGRVKKVYIQG
EPGSRISPQDFDKWYVRNSDGDMVSFASFATGKWIYGSPKLEQYNGISAVEILGEPAPGYSSGDAMKAIEDIAARLPEGF
HISWTGLSFEERLSGSQAPALYALSLLIVFLCLAALYESWSIPFSVMLVVPLGVLGAVCATLLRGLGNDVFFQVGLLTTI
GLSAKNAILIVEFARELHEKEGLSIKEAAVEAARVRLRPIIMTSLAFVMGVIPLAVSTGASSGSKHAIGTGVVGGMITAT
ILAIFYIPLFYMLIAGFFSRRNKKSSDKTEAEQS

Sequences:

>Translated_354_residues
MIQARNQLLAEAAKSPALNMVRPNGMNDEPQFQILIDDEKVQAFKLSMSDVDNIMSAAWGSMYVNDFNDRGRVKKVYIQG
EPGSRISPQDFDKWYVRNSDGDMVSFASFATGKWIYGSPKLEQYNGISAVEILGEPAPGYSSGDAMKAIEDIAARLPEGF
HISWTGLSFEERLSGSQAPALYALSLLIVFLCLAALYESWSIPFSVMLVVPLGVLGAVCATLLRGLGNDVFFQVGLLTTI
GLSAKNAILIVEFARELHEKEGLSIKEAAVEAARVRLRPIIMTSLAFVMGVIPLAVSTGASSGSKHAIGTGVVGGMITAT
ILAIFYIPLFYMLIAGFFSRRNKKSSDKTEAEQS
>Mature_354_residues
MIQARNQLLAEAAKSPALNMVRPNGMNDEPQFQILIDDEKVQAFKLSMSDVDNIMSAAWGSMYVNDFNDRGRVKKVYIQG
EPGSRISPQDFDKWYVRNSDGDMVSFASFATGKWIYGSPKLEQYNGISAVEILGEPAPGYSSGDAMKAIEDIAARLPEGF
HISWTGLSFEERLSGSQAPALYALSLLIVFLCLAALYESWSIPFSVMLVVPLGVLGAVCATLLRGLGNDVFFQVGLLTTI
GLSAKNAILIVEFARELHEKEGLSIKEAAVEAARVRLRPIIMTSLAFVMGVIPLAVSTGASSGSKHAIGTGVVGGMITAT
ILAIFYIPLFYMLIAGFFSRRNKKSSDKTEAEQS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the MexB

COG id: COG0841

COG function: function code V; Cation/multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family [H]

Homologues:

Organism=Escherichia coli, GI1788814, Length=334, Percent_Identity=63.4730538922156, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1786667, Length=344, Percent_Identity=62.5, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1789666, Length=325, Percent_Identity=60.6153846153846, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1789930, Length=344, Percent_Identity=58.7209302325581, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1788391, Length=342, Percent_Identity=26.3157894736842, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788390, Length=331, Percent_Identity=28.0966767371601, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786788, Length=323, Percent_Identity=23.5294117647059, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001036
- InterPro:   IPR004764 [H]

Pfam domain/function: PF00873 ACR_tran [H]

EC number: NA

Molecular weight: Translated: 38384; Mature: 38384

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQARNQLLAEAAKSPALNMVRPNGMNDEPQFQILIDDEKVQAFKLSMSDVDNIMSAAWG
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCEEHEEECHHHHHHHHHHHHC
SMYVNDFNDRGRVKKVYIQGEPGSRISPQDFDKWYVRNSDGDMVSFASFATGKWIYGSPK
HHEEECCCCCCCEEEEEEECCCCCCCCCCCCCHHEEECCCCCEEEHHHHCCCCEEECCCC
LEQYNGISAVEILGEPAPGYSSGDAMKAIEDIAARLPEGFHISWTGLSFEERLSGSQAPA
CHHCCCCHHHHEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCHH
LYALSLLIVFLCLAALYESWSIPFSVMLVVPLGVLGAVCATLLRGLGNDVFFQVGLLTTI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GLSAKNAILIVEFARELHEKEGLSIKEAAVEAARVRLRPIIMTSLAFVMGVIPLAVSTGA
CCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SSGSKHAIGTGVVGGMITATILAIFYIPLFYMLIAGFFSRRNKKSSDKTEAEQS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure
MIQARNQLLAEAAKSPALNMVRPNGMNDEPQFQILIDDEKVQAFKLSMSDVDNIMSAAWG
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCEEHEEECHHHHHHHHHHHHC
SMYVNDFNDRGRVKKVYIQGEPGSRISPQDFDKWYVRNSDGDMVSFASFATGKWIYGSPK
HHEEECCCCCCCEEEEEEECCCCCCCCCCCCCHHEEECCCCCEEEHHHHCCCCEEECCCC
LEQYNGISAVEILGEPAPGYSSGDAMKAIEDIAARLPEGFHISWTGLSFEERLSGSQAPA
CHHCCCCHHHHEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCHH
LYALSLLIVFLCLAALYESWSIPFSVMLVVPLGVLGAVCATLLRGLGNDVFFQVGLLTTI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GLSAKNAILIVEFARELHEKEGLSIKEAAVEAARVRLRPIIMTSLAFVMGVIPLAVSTGA
CCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SSGSKHAIGTGVVGGMITATILAIFYIPLFYMLIAGFFSRRNKKSSDKTEAEQS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10187844 [H]