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The map label for this gene is metK [H]

Identifier: 218691127

GI number: 218691127

Start: 3409621

End: 3410772

Strand: Direct

Name: metK [H]

Synonym: ECED1_3471

Alternate gene names: 218691127

Gene position: 3409621-3410772 (Clockwise)

Preceding gene: 218691126

Following gene: 218691128

Centisome position: 65.45

GC content: 53.21

Gene sequence:

>1152_bases
TTGAACGATTACCTGTTTACCTCCGAATCCGTTGCCGAAGGGCATCCCGATAAAATGGCGGACCAAATTTCTGATGCCAT
TTTGGATGCGATACTCCTACAGGACCCGTGGGGTAAGGTTGCCTGTGAATGCTTGGTGAAAACTGGTGCCACTATTGTTG
CCGGAGAAATATCTACCCATGCCGCCGTGGATATTGAGAAGATTGTCCGCAACACCATAAAAGAGATTGGTTACGACCAT
TCGCGATTGGGGTTTGATGGCAATACCTGCTGTGTATTAAATATCCTTGGCAAACAGTCCGCCAATATTGCTGACGGCAT
TCGCGGCCACTCGATGGAAGAACTGGGGGCGGGCGATCAGGGCATCACCTTCGGTTACGCCTGTGATGAAACGTCGGAAC
TGATGCCCGCCACGCTGGTATACGCCCATCGTCTGATGGAGCGCCAGGCGCAGTTGCGTAAATCCCAACGGTTACCATTT
TTACTCCCTGATGCCAAAAGCCAGGTTACCCTGCGCTATCAGGATAATCGTGTTCATTCAGTAGATACCGTTGTGGTGTC
TACCCAACACAGCCCTGACGTTTCTCTGGACGCGCTACGGGAAGCGGTCATCGAAGAAATTGTTAAACCTGTGATGCCTT
CGCACTGGCTCACACCACAAACCCGCTTTCTGGTGAACCCTGCAGGCAGTTTTGTGATTGGCGGGCCGGTGGGTGACTGC
GGGCTAACGGGGCGTAAAATTATTGTTGATACCTATGGCGGCGCGGCTTGCCATGGCGGCGGCGCGTTTTCAGGCAAAGA
TCCGTCGAAAGTCGATCGTTCCGCCGCCTACGCTGCTCGCTATGTGGCGAAAAACATCGTCGCCGCTGGGCTTGCCTCAC
GCTGTGAAGTCCAGTTGGGATGGGCCATCGGCCTGCCCCGCCCTGTTTCCGTCAGGATTAACACCTTCGGTACACAAACA
GTGTCATCTGAAGCGTTGCTGAGAGGCGTTAACAGCCATTTCGATCTTAGCGTGTTCGGCATCATTACTACGCTTGATCT
GCTGGTCCCCCGCTATCGTAAAACAGCCTGCTATGGTCACTTTGGTCGCGACAGCTTTCCGTGGGAAGTTACGGACAAAG
CGGCGCTATTATACGAAGACGTTGGACGTTAA

Upstream 100 bases:

>100_bases
GAAAGCAAAATAGTCCAACACGCCCGCAAGATAGTTTACGCCCACTTGTCAGGTTCCTATCCTAAGATAGGAATAAACTT
CCCAAACAAGTGGGCTCGCT

Downstream 100 bases:

>100_bases
GCCGACGACGCGCAGCACAGTTGAATTTATTACAGGAGAAATTTATGTCTGGGAAAAGTTTAGGCTTAATCGAAACCGTC
GGGATGTCTGCGGCGGTAGA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MNDYLFTSESVAEGHPDKMADQISDAILDAILLQDPWGKVACECLVKTGATIVAGEISTHAAVDIEKIVRNTIKEIGYDH
SRLGFDGNTCCVLNILGKQSANIADGIRGHSMEELGAGDQGITFGYACDETSELMPATLVYAHRLMERQAQLRKSQRLPF
LLPDAKSQVTLRYQDNRVHSVDTVVVSTQHSPDVSLDALREAVIEEIVKPVMPSHWLTPQTRFLVNPAGSFVIGGPVGDC
GLTGRKIIVDTYGGAACHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLASRCEVQLGWAIGLPRPVSVRINTFGTQT
VSSEALLRGVNSHFDLSVFGIITTLDLLVPRYRKTACYGHFGRDSFPWEVTDKAALLYEDVGR

Sequences:

>Translated_383_residues
MNDYLFTSESVAEGHPDKMADQISDAILDAILLQDPWGKVACECLVKTGATIVAGEISTHAAVDIEKIVRNTIKEIGYDH
SRLGFDGNTCCVLNILGKQSANIADGIRGHSMEELGAGDQGITFGYACDETSELMPATLVYAHRLMERQAQLRKSQRLPF
LLPDAKSQVTLRYQDNRVHSVDTVVVSTQHSPDVSLDALREAVIEEIVKPVMPSHWLTPQTRFLVNPAGSFVIGGPVGDC
GLTGRKIIVDTYGGAACHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLASRCEVQLGWAIGLPRPVSVRINTFGTQT
VSSEALLRGVNSHFDLSVFGIITTLDLLVPRYRKTACYGHFGRDSFPWEVTDKAALLYEDVGR
>Mature_383_residues
MNDYLFTSESVAEGHPDKMADQISDAILDAILLQDPWGKVACECLVKTGATIVAGEISTHAAVDIEKIVRNTIKEIGYDH
SRLGFDGNTCCVLNILGKQSANIADGIRGHSMEELGAGDQGITFGYACDETSELMPATLVYAHRLMERQAQLRKSQRLPF
LLPDAKSQVTLRYQDNRVHSVDTVVVSTQHSPDVSLDALREAVIEEIVKPVMPSHWLTPQTRFLVNPAGSFVIGGPVGDC
GLTGRKIIVDTYGGAACHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLASRCEVQLGWAIGLPRPVSVRINTFGTQT
VSSEALLRGVNSHFDLSVFGIITTLDLLVPRYRKTACYGHFGRDSFPWEVTDKAALLYEDVGR

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=375, Percent_Identity=55.4666666666667, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI4557737, Length=378, Percent_Identity=54.7619047619048, Blast_Score=390, Evalue=1e-108,
Organism=Escherichia coli, GI1789311, Length=382, Percent_Identity=63.6125654450262, Blast_Score=513, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17538498, Length=374, Percent_Identity=56.951871657754, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17538494, Length=374, Percent_Identity=56.1497326203209, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=55.5263157894737, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17551082, Length=376, Percent_Identity=54.7872340425532, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32565905, Length=374, Percent_Identity=50.5347593582888, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=54.601226993865, Blast_Score=331, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI25145635, Length=330, Percent_Identity=53.030303030303, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=53.4883720930233, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI32565909, Length=134, Percent_Identity=47.0149253731343, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=79.4871794871795, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=375, Percent_Identity=53.0666666666667, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323209, Length=371, Percent_Identity=53.099730458221, Blast_Score=375, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=55.6149732620321, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=55.6149732620321, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=55.6149732620321, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=55.6149732620321, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=55.6149732620321, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=55.6149732620321, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=55.0802139037433, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=55.0802139037433, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580541, Length=361, Percent_Identity=54.016620498615, Blast_Score=341, Evalue=4e-94,
Organism=Drosophila melanogaster, GI24580539, Length=118, Percent_Identity=58.4745762711864, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41437; Mature: 41437

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYLFTSESVAEGHPDKMADQISDAILDAILLQDPWGKVACECLVKTGATIVAGEISTH
CCCCEECCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCH
AAVDIEKIVRNTIKEIGYDHSRLGFDGNTCCVLNILGKQSANIADGIRGHSMEELGAGDQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHCCCCCCHHHHCCCCC
GITFGYACDETSELMPATLVYAHRLMERQAQLRKSQRLPFLLPDAKSQVTLRYQDNRVHS
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCEEE
VDTVVVSTQHSPDVSLDALREAVIEEIVKPVMPSHWLTPQTRFLVNPAGSFVIGGPVGDC
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAACHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLASRCEVQLG
CCCCCEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEE
WAIGLPRPVSVRINTFGTQTVSSEALLRGVNSHFDLSVFGIITTLDLLVPRYRKTACYGH
EEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
FGRDSFPWEVTDKAALLYEDVGR
CCCCCCCEEECCCHHHHHHHCCC
>Mature Secondary Structure
MNDYLFTSESVAEGHPDKMADQISDAILDAILLQDPWGKVACECLVKTGATIVAGEISTH
CCCCEECCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCH
AAVDIEKIVRNTIKEIGYDHSRLGFDGNTCCVLNILGKQSANIADGIRGHSMEELGAGDQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHCCCCCCHHHHCCCCC
GITFGYACDETSELMPATLVYAHRLMERQAQLRKSQRLPFLLPDAKSQVTLRYQDNRVHS
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCEEE
VDTVVVSTQHSPDVSLDALREAVIEEIVKPVMPSHWLTPQTRFLVNPAGSFVIGGPVGDC
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAACHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLASRCEVQLG
CCCCCEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEE
WAIGLPRPVSVRINTFGTQTVSSEALLRGVNSHFDLSVFGIITTLDLLVPRYRKTACYGH
EEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
FGRDSFPWEVTDKAALLYEDVGR
CCCCCCCEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA