Click here to switch to the map view.

The map label for this gene is ybiW [H]

Identifier: 218691119

GI number: 218691119

Start: 3400414

End: 3402966

Strand: Direct

Name: ybiW [H]

Synonym: ECED1_3461

Alternate gene names: 218691119

Gene position: 3400414-3402966 (Clockwise)

Preceding gene: 218691118

Following gene: 218691120

Centisome position: 65.27

GC content: 51.94

Gene sequence:

>2553_bases
ATGTTAGAGAAAGGCTTTTCTAATCCGACAGACCGTGTTGTAAGACTGAGAAATATGATCCTGACGGCGAAGCCGTATGT
GGAATCAGAACGCGCGGTGTTAGCAACCGAAGCATATAAAGAAACAGAACAACTGCCCGCGATTATGCGTCGTGCTAAAG
TGGTGGAAAAAATCTTTAACCAATTACCGGTAACAATTCGCCCAGACGAATTAATCGTTGGAGCGGTAACTATTAATCCT
CGCTCAACTGAAATCTGCCCTGAGTTCTCCTACGACTGGGTGGAAAAAGAGTTTGAGACTATGGAACACCGTATTGCCGA
CCCGTTTGTTATCCCGAAAAAGACTGCTCAGGAGCTTCATGAAGCATTTAAATACTGGCCGGGTAAAACTACCAGTGCTC
TGGCGGCATCTTACATGTCAGAAGGCACTAAAGAGAGTATGGCGAGCGGTGTATTCACTGTTGGTAACTACTTCTTCGGG
GGCGTCGGTCATGTCAGTGTTGACTACGGTAAAGTGCTGAAAATTGGTTTCCGTGGTATCATTAATGAAGTTAGCCGTGC
CTTGGAATCATTAGATCGCACTGAACCAGGATATATAAAGAAAGAACAGTTCTATAATGCGGTGCTGATCAGTTATAACG
CAGCGATCCGTTTCGCCCATCGTTACGCTGAAGAAGCATCCCGTCTGGCGCAGCAGGAGAGCAATCCGACCCGTAAACGC
GAACTGGAGCAGATTGCTCAAAACTGTACTCGCGTGCCGGAATACGGTGCTACCACATTCTGGGAAGCATGCCAGACATT
CTGGTTTATTCAGAGCATGTTACAGATTGAATCCAGCGGTCATTCTATCTCCCCGGGGCGTTTCGACCAGTATATGTACC
CGTATCTGGAGTCGGACAAATCTATCTCGCGTGAATTCGCGCAGGAACTGGTGGATTGCTGCTGGATCAAGCTTAACGAC
ATTAACAAAACGCGTGACGAAGTCTCTGCCCAGGCGTTTGCCGGTTACGCAGTGTTCCAGAACCTGTGCTGTGGCGGCCA
GACAGAAGACGGTCGCGACGCAACTAACGATCTGAGCTACATGTGTATGGAAGCGACTGCCCATGTGCGTCTGCCGCAGC
CATCCTTCTCAATCCGCGTATGGCAGGGTACTCCGGATGAGTTTCTGTATCGCGCTTGCGAGCTGGTGCGTATGGGCCTG
GGCGTTCCGGCAATGTACAACGATGAAGTAATCATCCCGGCGCTGCAGAACCGTGGCATTTCGCTGCGTGATGCGCGTGA
TTACTGTATCATCGGCTGCGTAGAGCCGCAGGCACCGCATCGCACCGAGGGCTGGCATGACGCCGCGTTCTTTAACGTTG
CAAAAGTGCTGGAAATCACCCTGAACAACGGTCGCGTTGGCAATAAACAGCTAGGCCCAGTGACTGGCGAACTGACTCAG
TTCACCAGCATGGAGGACTTCTACACCGCGTTCCAGAAACAGATGGCGCACTTCGTTCATCAACTGGTTGAAGCCTGTAA
CAGCGTGGATATTGCCCACGGCGAACGCTGCCCGCTGCCGTTCCTATCAGCACTGGTCGACGACTGTATCGGCCGTGGTA
AATCTCTCCAGGAAGGCGGGGCGATTTACAACTTTACTGGGCCGCAAGCATTCGGCGTAGCCGATACCGGCGACTCCGTC
TATGCCATCCAGAAACAGGTATTCGAAGATCGTAAACTGTCTTTGAGCGAACTGAAAAGCGCACTGGACGCCAACTTCGG
TTATCCGGTGGGTGCGAACCCGCACACTCCAGCGGCGAAATCCTCGCTCAACGAGCAGGATATCTACGATGTGGTGAAAC
GCATCATTGAACAGCATGGCGCGCTGGATCCGGCAGCGATTAAAAACGAAGTTTATCGCCAGCTGACTTCCGGCAGTGCA
GCACCGGTGCAATCCGGCACTATGTCCCGTCACGAGGAGATCCGTCGTATTCTGGAAAACACACCGTGCTTCGGTAACGA
CATCGATGACGTGGATTTAGTAGCGCGTAAATGTGCGCTGATCTACTGCCAGGAAGTTGAGAAATATACCAACCCGCGCG
GTGGCCAGTTCCAGGCTGGTATCTATCCGGTGTCTGCGAACGTGCTGTTTGGTAAAGACGTTGCCGCACTGCCGGATGGC
CGTCTGGCGAAAGAGCCGTTGGCGGATGGCGTATCTCCACGTCAGGGTAAAGACACCCTTGGCCCAACCGCCGCAGCCAA
CTCAGTTGCCAAACTGGATCACTTTATCGCCTCCAACGGTACGCTCTATAATCAGAAATTCCTGCCGTCTTCTTTGGCTG
GCGAGAACGGTCTGCGCAACTTCAGCGGCCTGATACGTCACTACTTCGATAAGAAAGGGATGCACGTTCAATTTAACGTT
ATCGACCGCAACACTTTGATCGAAGCCCAGAAAAATCCTGAGCAACATCAGGATCTGGTGGTACGCGTTGCGGGCTATAG
CGCCCAGTGGGTAGTTTTAGCGAAAGAAGTTCAGGACGACATAATCAGCCGTACTGAGCAACAACTGTCGTAA

Upstream 100 bases:

>100_bases
AGCAATATTACGTCACTTTATTACTCAAATGGGCACTAGCATTATTCATTAATACCCTGGCATCTCCGCAAGGTTAACAA
AATATATTCGGGAGTTTTAT

Downstream 100 bases:

>100_bases
ATACCAGACGGGCTATGTATCAATATATAGCCCGTTACTTTCTCCACGACTTCTGGTTGTTACTTTTATCTGAAAAGGTA
ATTCATCTGGTAAATATAGA

Product: putative Formate C-acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 3 [H]

Number of amino acids: Translated: 850; Mature: 850

Protein sequence:

>850_residues
MLEKGFSNPTDRVVRLRNMILTAKPYVESERAVLATEAYKETEQLPAIMRRAKVVEKIFNQLPVTIRPDELIVGAVTINP
RSTEICPEFSYDWVEKEFETMEHRIADPFVIPKKTAQELHEAFKYWPGKTTSALAASYMSEGTKESMASGVFTVGNYFFG
GVGHVSVDYGKVLKIGFRGIINEVSRALESLDRTEPGYIKKEQFYNAVLISYNAAIRFAHRYAEEASRLAQQESNPTRKR
ELEQIAQNCTRVPEYGATTFWEACQTFWFIQSMLQIESSGHSISPGRFDQYMYPYLESDKSISREFAQELVDCCWIKLND
INKTRDEVSAQAFAGYAVFQNLCCGGQTEDGRDATNDLSYMCMEATAHVRLPQPSFSIRVWQGTPDEFLYRACELVRMGL
GVPAMYNDEVIIPALQNRGISLRDARDYCIIGCVEPQAPHRTEGWHDAAFFNVAKVLEITLNNGRVGNKQLGPVTGELTQ
FTSMEDFYTAFQKQMAHFVHQLVEACNSVDIAHGERCPLPFLSALVDDCIGRGKSLQEGGAIYNFTGPQAFGVADTGDSV
YAIQKQVFEDRKLSLSELKSALDANFGYPVGANPHTPAAKSSLNEQDIYDVVKRIIEQHGALDPAAIKNEVYRQLTSGSA
APVQSGTMSRHEEIRRILENTPCFGNDIDDVDLVARKCALIYCQEVEKYTNPRGGQFQAGIYPVSANVLFGKDVAALPDG
RLAKEPLADGVSPRQGKDTLGPTAAANSVAKLDHFIASNGTLYNQKFLPSSLAGENGLRNFSGLIRHYFDKKGMHVQFNV
IDRNTLIEAQKNPEQHQDLVVRVAGYSAQWVVLAKEVQDDIISRTEQQLS

Sequences:

>Translated_850_residues
MLEKGFSNPTDRVVRLRNMILTAKPYVESERAVLATEAYKETEQLPAIMRRAKVVEKIFNQLPVTIRPDELIVGAVTINP
RSTEICPEFSYDWVEKEFETMEHRIADPFVIPKKTAQELHEAFKYWPGKTTSALAASYMSEGTKESMASGVFTVGNYFFG
GVGHVSVDYGKVLKIGFRGIINEVSRALESLDRTEPGYIKKEQFYNAVLISYNAAIRFAHRYAEEASRLAQQESNPTRKR
ELEQIAQNCTRVPEYGATTFWEACQTFWFIQSMLQIESSGHSISPGRFDQYMYPYLESDKSISREFAQELVDCCWIKLND
INKTRDEVSAQAFAGYAVFQNLCCGGQTEDGRDATNDLSYMCMEATAHVRLPQPSFSIRVWQGTPDEFLYRACELVRMGL
GVPAMYNDEVIIPALQNRGISLRDARDYCIIGCVEPQAPHRTEGWHDAAFFNVAKVLEITLNNGRVGNKQLGPVTGELTQ
FTSMEDFYTAFQKQMAHFVHQLVEACNSVDIAHGERCPLPFLSALVDDCIGRGKSLQEGGAIYNFTGPQAFGVADTGDSV
YAIQKQVFEDRKLSLSELKSALDANFGYPVGANPHTPAAKSSLNEQDIYDVVKRIIEQHGALDPAAIKNEVYRQLTSGSA
APVQSGTMSRHEEIRRILENTPCFGNDIDDVDLVARKCALIYCQEVEKYTNPRGGQFQAGIYPVSANVLFGKDVAALPDG
RLAKEPLADGVSPRQGKDTLGPTAAANSVAKLDHFIASNGTLYNQKFLPSSLAGENGLRNFSGLIRHYFDKKGMHVQFNV
IDRNTLIEAQKNPEQHQDLVVRVAGYSAQWVVLAKEVQDDIISRTEQQLS
>Mature_850_residues
MLEKGFSNPTDRVVRLRNMILTAKPYVESERAVLATEAYKETEQLPAIMRRAKVVEKIFNQLPVTIRPDELIVGAVTINP
RSTEICPEFSYDWVEKEFETMEHRIADPFVIPKKTAQELHEAFKYWPGKTTSALAASYMSEGTKESMASGVFTVGNYFFG
GVGHVSVDYGKVLKIGFRGIINEVSRALESLDRTEPGYIKKEQFYNAVLISYNAAIRFAHRYAEEASRLAQQESNPTRKR
ELEQIAQNCTRVPEYGATTFWEACQTFWFIQSMLQIESSGHSISPGRFDQYMYPYLESDKSISREFAQELVDCCWIKLND
INKTRDEVSAQAFAGYAVFQNLCCGGQTEDGRDATNDLSYMCMEATAHVRLPQPSFSIRVWQGTPDEFLYRACELVRMGL
GVPAMYNDEVIIPALQNRGISLRDARDYCIIGCVEPQAPHRTEGWHDAAFFNVAKVLEITLNNGRVGNKQLGPVTGELTQ
FTSMEDFYTAFQKQMAHFVHQLVEACNSVDIAHGERCPLPFLSALVDDCIGRGKSLQEGGAIYNFTGPQAFGVADTGDSV
YAIQKQVFEDRKLSLSELKSALDANFGYPVGANPHTPAAKSSLNEQDIYDVVKRIIEQHGALDPAAIKNEVYRQLTSGSA
APVQSGTMSRHEEIRRILENTPCFGNDIDDVDLVARKCALIYCQEVEKYTNPRGGQFQAGIYPVSANVLFGKDVAALPDG
RLAKEPLADGVSPRQGKDTLGPTAAANSVAKLDHFIASNGTLYNQKFLPSSLAGENGLRNFSGLIRHYFDKKGMHVQFNV
IDRNTLIEAQKNPEQHQDLVVRVAGYSAQWVVLAKEVQDDIISRTEQQLS

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787044, Length=864, Percent_Identity=33.4490740740741, Blast_Score=461, Evalue=1e-130,
Organism=Escherichia coli, GI1790388, Length=587, Percent_Identity=35.9454855195911, Blast_Score=398, Evalue=1e-111,
Organism=Escherichia coli, GI1787131, Length=199, Percent_Identity=34.6733668341709, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI48994926, Length=196, Percent_Identity=33.6734693877551, Blast_Score=116, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 94884; Mature: 94884

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKGFSNPTDRVVRLRNMILTAKPYVESERAVLATEAYKETEQLPAIMRRAKVVEKIFN
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLPVTIRPDELIVGAVTINPRSTEICPEFSYDWVEKEFETMEHRIADPFVIPKKTAQELH
HCCCEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
EAFKYWPGKTTSALAASYMSEGTKESMASGVFTVGNYFFGGVGHVSVDYGKVLKIGFRGI
HHHHHCCCCCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCCEEEECHHHHHHHHHHHH
INEVSRALESLDRTEPGYIKKEQFYNAVLISYNAAIRFAHRYAEEASRLAQQESNPTRKR
HHHHHHHHHHHHCCCCCCEEHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ELEQIAQNCTRVPEYGATTFWEACQTFWFIQSMLQIESSGHSISPGRFDQYMYPYLESDK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHCCCH
SISREFAQELVDCCWIKLNDINKTRDEVSAQAFAGYAVFQNLCCGGQTEDGRDATNDLSY
HHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
MCMEATAHVRLPQPSFSIRVWQGTPDEFLYRACELVRMGLGVPAMYNDEVIIPALQNRGI
HHHHHHHEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCEEEEHHHCCCC
SLRDARDYCIIGCVEPQAPHRTEGWHDAAFFNVAKVLEITLNNGRVGNKQLGPVTGELTQ
EEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHH
FTSMEDFYTAFQKQMAHFVHQLVEACNSVDIAHGERCPLPFLSALVDDCIGRGKSLQEGG
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
AIYNFTGPQAFGVADTGDSVYAIQKQVFEDRKLSLSELKSALDANFGYPVGANPHTPAAK
CEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHH
SSLNEQDIYDVVKRIIEQHGALDPAAIKNEVYRQLTSGSAAPVQSGTMSRHEEIRRILEN
HCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
TPCFGNDIDDVDLVARKCALIYCQEVEKYTNPRGGQFQAGIYPVSANVLFGKDVAALPDG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCEEECCCCCCCCCC
RLAKEPLADGVSPRQGKDTLGPTAAANSVAKLDHFIASNGTLYNQKFLPSSLAGENGLRN
CCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCHHHCCCCCHHH
FSGLIRHYFDKKGMHVQFNVIDRNTLIEAQKNPEQHQDLVVRVAGYSAQWVVLAKEVQDD
HHHHHHHHHCCCCCEEEEEEECCCCEEECCCCCHHHHCEEEEEECCCCEEEEHHHHHHHH
IISRTEQQLS
HHHHHHHHCC
>Mature Secondary Structure
MLEKGFSNPTDRVVRLRNMILTAKPYVESERAVLATEAYKETEQLPAIMRRAKVVEKIFN
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLPVTIRPDELIVGAVTINPRSTEICPEFSYDWVEKEFETMEHRIADPFVIPKKTAQELH
HCCCEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
EAFKYWPGKTTSALAASYMSEGTKESMASGVFTVGNYFFGGVGHVSVDYGKVLKIGFRGI
HHHHHCCCCCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCCEEEECHHHHHHHHHHHH
INEVSRALESLDRTEPGYIKKEQFYNAVLISYNAAIRFAHRYAEEASRLAQQESNPTRKR
HHHHHHHHHHHHCCCCCCEEHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ELEQIAQNCTRVPEYGATTFWEACQTFWFIQSMLQIESSGHSISPGRFDQYMYPYLESDK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHCCCH
SISREFAQELVDCCWIKLNDINKTRDEVSAQAFAGYAVFQNLCCGGQTEDGRDATNDLSY
HHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
MCMEATAHVRLPQPSFSIRVWQGTPDEFLYRACELVRMGLGVPAMYNDEVIIPALQNRGI
HHHHHHHEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCEEEEHHHCCCC
SLRDARDYCIIGCVEPQAPHRTEGWHDAAFFNVAKVLEITLNNGRVGNKQLGPVTGELTQ
EEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHH
FTSMEDFYTAFQKQMAHFVHQLVEACNSVDIAHGERCPLPFLSALVDDCIGRGKSLQEGG
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
AIYNFTGPQAFGVADTGDSVYAIQKQVFEDRKLSLSELKSALDANFGYPVGANPHTPAAK
CEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHH
SSLNEQDIYDVVKRIIEQHGALDPAAIKNEVYRQLTSGSAAPVQSGTMSRHEEIRRILEN
HCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
TPCFGNDIDDVDLVARKCALIYCQEVEKYTNPRGGQFQAGIYPVSANVLFGKDVAALPDG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCEEECCCCCCCCCC
RLAKEPLADGVSPRQGKDTLGPTAAANSVAKLDHFIASNGTLYNQKFLPSSLAGENGLRN
CCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCHHHCCCCCHHH
FSGLIRHYFDKKGMHVQFNVIDRNTLIEAQKNPEQHQDLVVRVAGYSAQWVVLAKEVQDD
HHHHHHHHHCCCCCEEEEEEECCCCEEECCCCCHHHHCEEEEEECCCCEEEEHHHHHHHH
IISRTEQQLS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]